Crystal structure of EHEC intimin: Insights into the complementarity between EPEC and EHEC

PLoS ONE

Volumn 5, Issue 12, 2010, Pages

  Yi, Yong ^a,b,c   Ma, Ying ^a,b   Gao, Feng ^e   Mao, Xuhu ^a   Peng, Hao ^b   Feng, Youjun ^b,d   Fan, Zheng ^b   Wang, Guihua ^b,d   Guo, Gang ^a   Yan, Jinghua ^b   Zeng, Hao ^a   Zou, Quanming ^a   Gao, George F ^b,f  

^a THIRD MILITARY MEDICAL UNIVERSITY   (China)

^b INSTITUTE OF MICROBIOLOGY   (China)

^c 306 HOSPITAL OF PLA   (China)

^d UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^e INSTITUTE OF BIOPHYSICS   (China)

^f INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CELL SURFACE PROTEIN; INTIMIN; RECEPTOR; TRANSLOCATED INTIMIN RECEPTOR; UNCLASSIFIED DRUG; ADHESIN; EAEA PROTEIN, E COLI; ESCHERICHIA COLI PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL TRANSLOCATION; BINDING AFFINITY; BINDING SITE; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENTEROHEMORRHAGIC ESCHERICHIA COLI; ENTEROPATHOGENIC ESCHERICHIA COLI; NONHUMAN; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN LOCALIZATION; SEQUENCE ALIGNMENT; SPECIES DIFFERENCE; STRUCTURE ANALYSIS; AMINO ACID SEQUENCE; CHEMICAL STRUCTURE; CHEMISTRY; CONFORMATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; MUTATION; PROTEIN SECONDARY STRUCTURE; SEQUENCE HOMOLOGY; X RAY CRYSTALLOGRAPHY;

BACTERIA (MICROORGANISMS);

ADHESINS, BACTERIAL; AMINO ACID SEQUENCE; CLONING, MOLECULAR; CRYSTALLOGRAPHY, X-RAY; ENTEROHEMORRHAGIC ESCHERICHIA COLI; ENTEROPATHOGENIC ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MODELS, MOLECULAR; MOLECULAR CONFORMATION; MOLECULAR SEQUENCE DATA; MUTATION; PROTEIN BINDING; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 78650739868     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0015285     Document Type: Article

References (34)

1. Welinder-Olsson C, Kaijser B (2005) Enterohemorrhagic Escherichia coli (EHEC). Scand J Infect Dis 37: 405-416.
2. Bell C (2002) Approach to the control of entero-haemorrhagic Escherichia coli (EHEC). Int J Food Microbiol 78: 197-216.
3. Soderstrom A, Lindberg A, Andersson Y (2005) EHEC O157 outbreak in Sweden from locally produced lettuce, August-September 2005. Euro Surveill 10: E050922 050921.
4. La Ragione RM, Ahmed NM, Best A, Clifford D, Weyer U, et al. (2005) Colonization of 8-week-old conventionally reared goats by Escherichia coli O157: H7 after oral inoculation. J Med Microbiol 54: 485-492.
5. Mellmann A, Bielaszewska M, Zimmerhackl LB, Prager R, Harmsen D, et al. (2005) Enterohemorrhagic Escherichia coli in human infection: in vivo evolution of a bacterial pathogen. Clin Infect Dis 41: 785-792.
6. Nougayrede JP, Fernandes PJ, Donnenberg MS (2003) Adhesion of enteropathogenic Escherichia coli to host cells. Cell Microbiol 5: 359-372.
7. Henderson S, Jablensky A The Lundby Study. Aust N Z J Psychiatry 44: 1-3.
8. Watanabe Y, Ozasa K, Mermin JH, Griffin PM, Masuda K, et al. (1999) Factory outbreak of Escherichia coli O157:H7 infection in Japan. Emerg Infect Dis 5: 424-428.
9. Wang H, Jing HQ, Li HW, Ni DX, Zhao GF, et al. (2004) [Epidemiological study on an outbreak caused by E. coli O157:H7 in Jiangsu province.]. Zhonghua Liu Xing Bing Xue Za Zhi 25: 938-940.
10. Mead PS, Slutsker L, Dietz V, McCaig LF, Bresee JS, et al. (1999) Foodrelated illness and death in the United States. Emerg Infect Dis 5: 607-625.
11. Babol-Pokora K, Prosniak A, Jacewicz R, Berent J (2009) [The usefulness of SNP markers for analyses of highly degraded biological materials]. Arch Med Sadowej Kryminol 59: 118-123.
12. Liu H, Radhakrishnan P, Magoun L, Prabu M, Campellone KG, et al. (2002) Point mutants of EHEC intimin that diminish Tir recognition and actin pedestal formation highlight a putative Tir binding pocket. Mol Microbiol 45: 1557-1573.
13. Spears KJ, Roe AJ, Gally DL (2006) A comparison of enteropathogenic and enterohaemorrhagic Escherichia coli pathogenesis. FEMS Microbiol Lett 255: 187-202.
14. O'Brien AD, Tesh VL, Donohue-Rolfe A, Jackson MP, Olsnes S, et al. (1992) Shiga toxin: biochemistry, genetics, mode of action, and role in pathogenesis. Curr Top Microbiol Immunol 180: 65-94.
15. Donnenberg MS, Tzipori S, McKee ML, O'Brien AD, Alroy J, et al. (1993) The role of the eae gene of enterohemorrhagic Escherichia coli in intimate attachment in vitro and in a porcine model. J Clin Invest 92: 1418-1424.
16. Perna NT, Mayhew GF, Posfai G, Elliott S, Donnenberg MS, et al. (1998) Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7. Infect Immun 66: 3810-3817.
17. DeVinney R, Stein M, Reinscheid D, Abe A, Ruschkowski S, et al. (1999) Enterohemorrhagic Escherichia coli O157:H7 produces Tir, which is translocated to the host cell membrane but is not tyrosine phosphorylated. Infect Immun 67: 2389-2398.
18. Jarvis KG, Kaper JB (1996) Secretion of extracellular proteins by enterohemorrhagic Escherichia coli via a putative type III secretion system. Infect Immun 64: 4826-4829.
19. Kenny B, DeVinney R, Stein M, Reinscheid DJ, Frey EA, et al. (1997) Enteropathogenic E. coli (EPEC) transfers its receptor for intimate adherence into mammalian cells. Cell 91: 511-520.
20. Knutton S, Baldwin T, Williams PH, McNeish AS (1989) Actin accumulation at sites of bacterial adhesion to tissue culture cells: basis of a new diagnostic test for enteropathogenic and enterohemorrhagic Escherichia coli. Infect Immun 57: 1290-1298.
21. Kenny B (1999) Phosphorylation of tyrosine 474 of the enteropathogenic Escherichia coli (EPEC) Tir receptor molecule is essential for actin nucleating activity and is preceded by additional host modifications. Mol Microbiol 31: 1229-1241.
22. Celli J, Deng W, Finlay BB (2000) Enteropathogenic Escherichia coli (EPEC) attachment to epithelial cells: exploiting the host cell cytoskeleton from the outside. Cell Microbiol 2: 1-9.
23. Adu-Bobie J, Frankel G, Bain C, Goncalves AG, Trabulsi LR, et al. (1998) Detection of intimins alpha, beta, gamma, and delta, four intimin derivatives expressed by attaching and effacing microbial pathogens. J Clin Microbiol 36: 662-668.
24. Oswald E, Schmidt H, Morabito S, Karch H, Marches O, et al. (2000) Typing of intimin genes in human and animal enterohemorrhagic and enteropathogenic Escherichia coli: characterization of a new intimin variant. Infect Immun 68: 64-71.
25. Batchelor M, Prasannan S, Daniell S, Reece S, Connerton I, et al. (2000) Structural basis for recognition of the translocated intimin receptor (Tir) by intimin from enteropathogenic Escherichia coli. EMBO J 19: 2452-2464.
26. Luo Y, Frey EA, Pfuetzner RA, Creagh AL, Knoechel DG, et al. (2000) Crystal structure of enteropathogenic Escherichia coli intimin-receptor complex. Nature 405: 1073-1077.
27. Holm L, Rosenstrom P (2010) Dali server: conservation mapping in 3D. Nucleic Acids Res 38 Suppl: W545-549.
28. Garboczi DN, Hung DT, Wiley DC (1992) HLA-A2-peptide complexes: refolding and crystallization of molecules expressed in Escherichia coli and complexed with single antigenic peptides. Proc Natl Acad Sci U S A 89: 3429-3433.
29. Zhou M, Xu Y, Lou Z, Cole DK, Li X, et al. (2004) Complex assembly, crystallization and preliminary X-ray crystallographic studies of MHC H-2Kd complexed with an HBV-core nonapeptide. Acta Crystallogr D Biol Crystallogr 60: 1473-1475.
30. Anonymus (2009) CrystalClear and CrystalStrcuture. Tokyo, Japan: Rigaku Corporation.
31. Brunger AT, Adams PD, Clore GM, DeLano WL, Gros P, et al. (1998) Crystallography & NMR system: A new software suite for macromolecular structure determination. Acta Crystallogr D Biol Crystallogr 54: 905-921.
32. Laskowski R, MacArthur M, Moss D, Thornton J (1993) PROCHECK: A program to check the stereochemical quality of protein structures. J Appl Cryst 26: 283-291.
33. Anonymus (1994) The CCP4 suite: programs for protein crystallography. Acta Crystallogr D Biol Crystallogr 50: 760-763.
34. Emsley P, Cowtan K (2004) Coot: model-building tools for molecular graphics. Acta Crystallogr D Biol Crystallogr 60: 2126-2132.