Characterization and molecular modelling of an engineered organic solvent tolerant, thermostable lipase with enhanced enzyme activity

Journal of Molecular Catalysis B: Enzymatic

Volumn 97, Issue , 2013, Pages 243-251

  Kumar, Rakesh ^a   Singh, Ranvir ^a   Kaur, Jagdeep ^a  

^a PANJAB UNIVERSITY   (India)

Author keywords

Circular dichroism; Fluorescence; Lipase; Molecular modelling; Organic solvent tolerant; Thermostability

Indexed keywords

CATALYTIC EFFICIENCIES; ENHANCED ENZYME ACTIVITY; ERROR-PRONE MUTAGENESIS; SOLVENT TOLERANT; STEADY STATE FLUORESCENCES; STRUCTURAL STABILITIES; TEMPERATURE DEPENDENT; THERMOSTABILITY;

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; CLONING; DICHROISM; ENZYMES; ESCHERICHIA COLI; FLUORESCENCE; HYDROPHOBICITY; LIPASES; MOLECULAR MODELING; ORGANIC SOLVENTS; PROTEINS;

STABILITY;

LIPR1 PROTEIN; LIPR3 PROTEIN; ORGANIC SOLVENT; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; CIRCULAR DICHROISM; CONTROLLED STUDY; ENZYMATIC ASSAY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME SPECIFICITY; ENZYME STABILITY; ESCHERICHIA COLI; FLUORESCENCE; GENE EXPRESSION; GENETIC VARIABILITY; MOLECULAR MODEL; NONHUMAN; NUCLEOTIDE SEQUENCE; POINT MUTATION; STEADY STATE;

EID: 84884516525     PISSN: 13811177     EISSN: 18733158     Source Type: Journal    
DOI: 10.1016/j.molcatb.2013.09.001     Document Type: Article

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