Optimization of a thermostable lipase from Bacillus stearothermophilus p1: Overexpression, purification, and characterization

Protein Expression and Purification

Volumn 22, Issue 3, 2001, Pages 388-398

  Sinchaikul, Supachok ^a,b   Sookkheo, Boonyaras ^b,c   Phutrakul, Suree ^a   Pan, Fu Ming ^b   Chen, Shui Tein ^b  

^a CHIANG MAI UNIVERSITY   (Thailand)

^b INSTITUTE OF BIOLOGICAL CHEMISTRY   (Taiwan)

^c RANGSIT UNIVERSITY   (Thailand)

Author keywords

Bacillus stearothermophilus; Cloning; Sequence; Thermostable lipase

Indexed keywords

AMINO ACID SEQUENCE; BACILLUS STEAROTHERMOPHILUS; BACTERIAL ENZYME; DNA FRAGMENT; ENZYME CHARACTERIZATION; ENZYME INACTIVATION; ENZYME PURIFICATION; ESCHERICHIA COLI; EXPRESSION VECTOR; GENETIC STRAIN; GENETIC TRANSFORMATION; MOLECULAR WEIGHT; OPEN READING FRAME; PH; SDS POLYACRYLAMIDE GEL ELECTROPHORESIS; SEQUENCE ANALYSIS; TRIACYLGLYCEROL LIPASE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; GEOBACILLUS STEAROTHERMOPHILUS;

EID: 0034864538     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1456     Document Type: Article

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