Substitution of Asp189 residue alters the activity and thermostability of Geobacillus sp. NTU 03 lipase

Biotechnology Letters

Volumn 33, Issue 9, 2011, Pages 1841-1846

  Shih, Tsung Wei ^a   Pan, Tzu Ming ^a  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

Author keywords

Geobacillus thermoalkalophilic lipase; Lid domain; Mutagenesis; Thermostability

Indexed keywords

CATALYTIC EFFICIENCIES; ERROR PRONE PCR; GEOBACILLUS THERMOALKALOPHILIC LIPASE; LID DOMAIN; N-HEXANE; THERMOACTIVITY; THERMOSTABILITY; TRANSESTERIFICATION ACTIVITY; WILD TYPES;

HEXANE; MUTAGENESIS;

LIPASES;

4-NITROPHENYL PALMITATE; BACTERIAL DNA; MUTANT PROTEIN; PALMITIC ACID 4 NITROPHENYL ESTER; PALMITIC ACID DERIVATIVE; TRIACYLGLYCEROL LIPASE;

AMINO ACID SUBSTITUTION; ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; DNA SEQUENCE; ENZYMOLOGY; GENETICS; GEOBACILLUS; HEAT; KINETICS; METABOLISM; METHODOLOGY; MISSENSE MUTATION; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROTEIN CONFORMATION; PROTEIN STABILITY;

AMINO ACID SUBSTITUTION; DNA, BACTERIAL; GEOBACILLUS; HOT TEMPERATURE; KINETICS; LIPASE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTANT PROTEINS; MUTATION, MISSENSE; PALMITATES; POLYMERASE CHAIN REACTION; PROTEIN CONFORMATION; PROTEIN STABILITY; SEQUENCE ANALYSIS, DNA;

GEOBACILLUS;

EID: 80051473765     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-011-0635-3     Document Type: Article

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