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Volumn 396, Issue 1-2, 2013, Pages 116-127

The two hit hypothesis: An improved method for siRNA-mediated gene silencing in stimulated primary human T cells

Author keywords

Gene silencing; Nucleofection; SiRNA; T cell; Transfection

Indexed keywords

CHEMOKINE; PROTEIN KINASE C BETA; SMALL INTERFERING RNA; TALIN;

EID: 84884350676     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2013.08.005     Document Type: Article
Times cited : (7)

References (40)
  • 1
    • 34248682403 scopus 로고    scopus 로고
    • RNAi therapeutics: principles, prospects and challenges
    • Aagaard L., Rossi J.J. RNAi therapeutics: principles, prospects and challenges. Adv. Drug Deliv. Rev. 2007, 59:75-86.
    • (2007) Adv. Drug Deliv. Rev. , vol.59 , pp. 75-86
    • Aagaard, L.1    Rossi, J.J.2
  • 4
    • 0038731005 scopus 로고    scopus 로고
    • Migration and T-lymphocyte effector function
    • Bradley L.M. Migration and T-lymphocyte effector function. Curr. Opin. Immunol. 2003, 15:343-348.
    • (2003) Curr. Opin. Immunol. , vol.15 , pp. 343-348
    • Bradley, L.M.1
  • 5
    • 84862576814 scopus 로고    scopus 로고
    • Phosphatase PPM1A negatively regulates P-TEFb function in resting CD4T+ T cells and inhibits HIV-1 gene expression
    • Budhiraja S., Ramakrishnan R., Rice A.P. Phosphatase PPM1A negatively regulates P-TEFb function in resting CD4T+ T cells and inhibits HIV-1 gene expression. Retrovirology 2012, 9:52.
    • (2012) Retrovirology , vol.9 , pp. 52
    • Budhiraja, S.1    Ramakrishnan, R.2    Rice, A.P.3
  • 6
    • 77952644251 scopus 로고    scopus 로고
    • Interleukin-7 mediates glucose utilization in lymphocytes through transcriptional regulation of the hexokinase II gene
    • Chehtane M., Khaled A.R. Interleukin-7 mediates glucose utilization in lymphocytes through transcriptional regulation of the hexokinase II gene. Am. J. Physiol. Cell Physiol. 2010, 298:C1560-C1571.
    • (2010) Am. J. Physiol. Cell Physiol. , vol.298
    • Chehtane, M.1    Khaled, A.R.2
  • 7
    • 72449192809 scopus 로고    scopus 로고
    • Guidelines for the effective use of chemical inhibitors of protein function to understand their roles in cell regulation
    • Cohen P. Guidelines for the effective use of chemical inhibitors of protein function to understand their roles in cell regulation. Biochem. J. 2010, 425:53-54.
    • (2010) Biochem. J. , vol.425 , pp. 53-54
    • Cohen, P.1
  • 8
    • 67649408951 scopus 로고    scopus 로고
    • SiRNA delivery using peptide transduction domains
    • Eguchi A., Dowdy S.F. siRNA delivery using peptide transduction domains. Trends Pharmacol. Sci. 2009, 30:341-345.
    • (2009) Trends Pharmacol. Sci. , vol.30 , pp. 341-345
    • Eguchi, A.1    Dowdy, S.F.2
  • 10
    • 84862619185 scopus 로고    scopus 로고
    • L-plastin regulates polarization and migration in chemokine-stimulated human T lymphocytes
    • Freeley M., O'Dowd F., Paul T., Kashanin D., Davies A., Kelleher D., Long A. L-plastin regulates polarization and migration in chemokine-stimulated human T lymphocytes. J. Immunol. 2012, 188:6357-6370.
    • (2012) J. Immunol. , vol.188 , pp. 6357-6370
    • Freeley, M.1    O'Dowd, F.2    Paul, T.3    Kashanin, D.4    Davies, A.5    Kelleher, D.6    Long, A.7
  • 11
    • 0037383050 scopus 로고    scopus 로고
    • Electroporation: theory and methods, perspectives for drug delivery, gene therapy and research
    • Gehl J. Electroporation: theory and methods, perspectives for drug delivery, gene therapy and research. Acta Physiol. Scand. 2003, 177:437-447.
    • (2003) Acta Physiol. Scand. , vol.177 , pp. 437-447
    • Gehl, J.1
  • 12
    • 62849094732 scopus 로고    scopus 로고
    • Selectivity and therapeutic inhibition of kinases: to be or not to be?
    • Ghoreschi K., Laurence A., O'Shea J.J. Selectivity and therapeutic inhibition of kinases: to be or not to be?. Nat. Immunol. 2009, 10:356-360.
    • (2009) Nat. Immunol. , vol.10 , pp. 356-360
    • Ghoreschi, K.1    Laurence, A.2    O'Shea, J.J.3
  • 13
    • 33645823860 scopus 로고    scopus 로고
    • Efficient nonviral gene delivery into primary lymphocytes from rats and mice
    • Goffinet C., Keppler O.T. Efficient nonviral gene delivery into primary lymphocytes from rats and mice. FASEB J. 2006, 20:500-502.
    • (2006) FASEB J. , vol.20 , pp. 500-502
    • Goffinet, C.1    Keppler, O.T.2
  • 15
    • 33845907631 scopus 로고    scopus 로고
    • A cost effective non-commercial ECL-solution for Western blot detections yielding strong signals and low background
    • Haan C., Behrmann I. A cost effective non-commercial ECL-solution for Western blot detections yielding strong signals and low background. J. Immunol. Methods 2007, 318:11-19.
    • (2007) J. Immunol. Methods , vol.318 , pp. 11-19
    • Haan, C.1    Behrmann, I.2
  • 17
    • 0036394242 scopus 로고    scopus 로고
    • Gene delivery into primary T cells: overview and characterization of a transgenic model for efficient adenoviral transduction
    • Hurez V., Hautton R.D., Oliver J., Matthews R.J., Weaver C.K. Gene delivery into primary T cells: overview and characterization of a transgenic model for efficient adenoviral transduction. Immunol. Res. 2002, 26:131-141.
    • (2002) Immunol. Res. , vol.26 , pp. 131-141
    • Hurez, V.1    Hautton, R.D.2    Oliver, J.3    Matthews, R.J.4    Weaver, C.K.5
  • 18
    • 70349438847 scopus 로고    scopus 로고
    • Engineering lymphocyte subsets: tools, trials and tribulations
    • June C.H., Blazar B.R., Riley J.L. Engineering lymphocyte subsets: tools, trials and tribulations. Nat. Rev. Immunol. 2009, 9:704-716.
    • (2009) Nat. Rev. Immunol. , vol.9 , pp. 704-716
    • June, C.H.1    Blazar, B.R.2    Riley, J.L.3
  • 20
    • 80055115046 scopus 로고    scopus 로고
    • Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro
    • Lefort C.T., Kim M. Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro. J. Vis. Exp. 2010, (40):e2017. 10.3791/2017.
    • (2010) J. Vis. Exp. , Issue.40
    • Lefort, C.T.1    Kim, M.2
  • 22
    • 34247582332 scopus 로고    scopus 로고
    • SiRNA delivery into human T cells and primary cells with carbon-nanotube transporters
    • Liu Z., Winters M., Holodniy M., Dai H. siRNA delivery into human T cells and primary cells with carbon-nanotube transporters. Angew. Chem. Int. Ed. Engl. 2007, 46:2023-2027.
    • (2007) Angew. Chem. Int. Ed. Engl. , vol.46 , pp. 2023-2027
    • Liu, Z.1    Winters, M.2    Holodniy, M.3    Dai, H.4
  • 27
    • 65349118108 scopus 로고    scopus 로고
    • Systemic siRNA delivery to leukocyte-implicated diseases
    • Peer D., Shimaoka M. Systemic siRNA delivery to leukocyte-implicated diseases. Cell Cycle 2009, 8:853-859.
    • (2009) Cell Cycle , vol.8 , pp. 853-859
    • Peer, D.1    Shimaoka, M.2
  • 28
    • 38849166053 scopus 로고    scopus 로고
    • Systemic leukocyte-directed siRNA delivery revealing cyclin D1 as an anti-inflammatory target
    • Peer D., Park E.J., Morishita Y., Carman C.V., Shimaoka M. Systemic leukocyte-directed siRNA delivery revealing cyclin D1 as an anti-inflammatory target. Science 2008, 319:627-630.
    • (2008) Science , vol.319 , pp. 627-630
    • Peer, D.1    Park, E.J.2    Morishita, Y.3    Carman, C.V.4    Shimaoka, M.5
  • 29
    • 0029976805 scopus 로고    scopus 로고
    • The use of dominant-negative mutations to elucidate signal transduction pathways in lymphocytes
    • Perlmutter R.M., Alberola-Ila J. The use of dominant-negative mutations to elucidate signal transduction pathways in lymphocytes. Curr. Opin. Immunol. 1996, 8:285-290.
    • (1996) Curr. Opin. Immunol. , vol.8 , pp. 285-290
    • Perlmutter, R.M.1    Alberola-Ila, J.2
  • 31
    • 84858758766 scopus 로고    scopus 로고
    • Adoptive immunotherapy for cancer: harnessing the T cell response
    • Restifo N.P., Dudley M.E., Rosenberg S.A. Adoptive immunotherapy for cancer: harnessing the T cell response. Nat. Rev. Immunol. 2012, 12:269-281.
    • (2012) Nat. Rev. Immunol. , vol.12 , pp. 269-281
    • Restifo, N.P.1    Dudley, M.E.2    Rosenberg, S.A.3
  • 32
    • 62849096347 scopus 로고    scopus 로고
    • Lymphocyte signaling: beyond knockouts
    • Saveliev A., Tybulewicz V.L. Lymphocyte signaling: beyond knockouts. Nat. Immunol. 2009, 10:361-364.
    • (2009) Nat. Immunol. , vol.10 , pp. 361-364
    • Saveliev, A.1    Tybulewicz, V.L.2
  • 33
    • 83555166124 scopus 로고    scopus 로고
    • Subcellular fate and off-target effects of siRNA, shRNA, and miRNA
    • Singh S., Narang A.S., Mahato R.I. Subcellular fate and off-target effects of siRNA, shRNA, and miRNA. Pharm. Res. 2011, 28:2996-3015.
    • (2011) Pharm. Res. , vol.28 , pp. 2996-3015
    • Singh, S.1    Narang, A.S.2    Mahato, R.I.3
  • 34
    • 35148819807 scopus 로고    scopus 로고
    • PI3Kgamma is the dominant isoform involved in migratory responses of human T lymphocytes: effects of ex vivo maintenance and limitations of non-viral delivery of siRNA
    • Smith L.D., Hickman E.S., Parry R.V., Westwick J., Ward S.G. PI3Kgamma is the dominant isoform involved in migratory responses of human T lymphocytes: effects of ex vivo maintenance and limitations of non-viral delivery of siRNA. Cell. Signal. 2007, 19:2528-2539.
    • (2007) Cell. Signal. , vol.19 , pp. 2528-2539
    • Smith, L.D.1    Hickman, E.S.2    Parry, R.V.3    Westwick, J.4    Ward, S.G.5
  • 36
    • 75749156214 scopus 로고    scopus 로고
    • New insights into T cell biology and T cell-directed therapy for autoimmunity, inflammation, and immunosuppression
    • Steward-Tharp S.M., Song Y.J., Siegel R.M., O'Shea J.J. New insights into T cell biology and T cell-directed therapy for autoimmunity, inflammation, and immunosuppression. Ann. N. Y. Acad. Sci. 2010, 1183:123-148.
    • (2010) Ann. N. Y. Acad. Sci. , vol.1183 , pp. 123-148
    • Steward-Tharp, S.M.1    Song, Y.J.2    Siegel, R.M.3    O'Shea, J.J.4
  • 37
    • 33644851858 scopus 로고    scopus 로고
    • Enrichment of nucleofected primary human CD4+ T cells: a novel and efficient method for studying gene function and role in human primary T helper cell differentiation
    • Tahvanainen J., Pykalainen M., Kallonen T., Lahteenmaki H., Rasool O., Lahesmaa R. Enrichment of nucleofected primary human CD4+ T cells: a novel and efficient method for studying gene function and role in human primary T helper cell differentiation. J. Immunol. Methods 2006, 310:30-39.
    • (2006) J. Immunol. Methods , vol.310 , pp. 30-39
    • Tahvanainen, J.1    Pykalainen, M.2    Kallonen, T.3    Lahteenmaki, H.4    Rasool, O.5    Lahesmaa, R.6
  • 38
    • 50049102160 scopus 로고    scopus 로고
    • How chemokines invite leukocytes to dance
    • Thelen M., Stein J.V. How chemokines invite leukocytes to dance. Nat. Immunol. 2008, 9:953-959.
    • (2008) Nat. Immunol. , vol.9 , pp. 953-959
    • Thelen, M.1    Stein, J.V.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.