siRNA stabilization prolongs gene knockdown in primary T lymphocytes

European Journal of Immunology

Volumn 38, Issue 9, 2008, Pages 2616-2625

  Mantei, Andrej ^a   Rutz, Sascha ^a   Janke, Marko ^a   Kirchhoff, Dennis ^a   Jung, Ulrike ^b   Patzel, Volker ^b   Vogel, Uwe ^b   Rudel, Thomas ^b   Andreou, Ioanna ^c   Weber, Martin ^c   Scheffold, Alexander ^a  

^a DEUTSCHES RHEUMA FORSCHUNGSZENTRUM   (Germany)

^b MAX PLANCK INSTITUTE FOR INFECTION BIOLOGY   (Germany)

^c QIAGEN GMBH   (Germany)

Author keywords

Gene expression; Immune regulation; T cells

Indexed keywords

CD4 ANTIGEN; NUCLEASE; SMALL INTERFERING RNA; TRANSCRIPTION FACTOR GATA 3; OVALBUMIN;

ANIMAL CELL; ARTICLE; CELL ACTIVATION; CELL FUNCTION; CHEMICAL MODIFICATION; CONTROLLED STUDY; ELECTROPORATION; ENZYME DEGRADATION; FUNCTIONAL GENOMICS; GENE EXPRESSION REGULATION; GENE SILENCING; GENE TARGETING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMUNOREGULATION; IN VITRO STUDY; IN VIVO STUDY; KNOCKOUT GENE; LYMPHOCYTE DIFFERENTIATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; RNA INTERFERENCE; RNA ISOLATION; RNA STABILITY; T LYMPHOCYTE; T LYMPHOCYTE ACTIVATION; ANIMAL; BAGG ALBINO MOUSE; C57BL MOUSE; CD4+ T LYMPHOCYTE; CELL CULTURE; CHEMISTRY; IMMUNOLOGY; METABOLISM; METHODOLOGY;

ANIMALS; CD4-POSITIVE T-LYMPHOCYTES; CELLS, CULTURED; ELECTROPORATION; HUMANS; MICE; MICE, INBRED BALB C; MICE, INBRED C57BL; OVALBUMIN; RNA INTERFERENCE; RNA STABILITY; RNA, SMALL INTERFERING; TRANSFECTION;

EID: 55249097026     PISSN: 00142980     EISSN: 15214141     Source Type: Journal    
DOI: 10.1002/eji.200738075     Document Type: Article

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