Bridging the gap: A GFP-based strategy for overexpression and purification of membrane proteins with intra and extracellular C-termini

Protein Science

Volumn 19, Issue 4, 2010, Pages 868-880

  Hsieh, Jennifer M ^a,b   Besserer, Gabriel Mercado ^b   Madej, M Gregor ^b   Bui, Ha Quyen ^b   Kwon, Seunghyug ^b   Abramson, Jeff ^b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Fluorescence detection size exclusion chromatography FSEC; Glycophorin A; Green fluorescent protein; Membrane protein expression; pWARF

Indexed keywords

GLYCOPHORIN A; GREEN FLUORESCENT PROTEIN; MEMBRANE PROTEIN;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STABILITY; VIBRIO PARAHAEMOLYTICUS;

CHROMATOGRAPHY, GEL; ESCHERICHIA COLI; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; MEMBRANE PROTEINS; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI;

EID: 77950229223     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.365     Document Type: Article

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