Green fluorescent protein functions as a reporter for protein localization in Escherichia coli

Journal of Bacteriology

Volumn 182, Issue 14, 2000, Pages 4068-4076

  Feilmeier, Bradley J ^a   Iseminger, Ginger ^a   Schroeder, Diane ^a   Webber, Hannali ^a   Phillips, Gregory J ^a  

^a IOWA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE; GREEN FLUORESCENT PROTEIN;

ARTICLE; CYTOPLASM; ESCHERICHIA COLI; GENE EXPRESSION; GENE FUNCTION; GENE MUTATION; GENE SEQUENCE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN LOCALIZATION; PROTEIN SYNTHESIS; REPORTER GENE;

AMINO ACID SEQUENCE; ATP-BINDING CASSETTE TRANSPORTERS; BACTERIAL PROTEINS; BASE SEQUENCE; BIOLOGICAL TRANSPORT; CARRIER PROTEINS; CELL COMPARTMENTATION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MOLECULAR SEQUENCE DATA; MONOSACCHARIDE TRANSPORT PROTEINS; MUTATION; PERIPLASM; PERIPLASMIC BINDING PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; PROKARYOTA;

EID: 0033917124     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.182.14.4068-4076.2000     Document Type: Article

References (56)

1. Bardwell, J. C. A., K. McGovern, and J. Beckwith. 1991. Identification of a protein required for disulfide bond formation in vivo. Cell 67:581-589.
2. Bassford, P., and J. Beckwith. 1979. Escherichia coli mutants accumulating the precursor of a secreted protein in the cytoplasm. Nature 277:538-541.
3. Bassford, P. J., Jr., T. J. Silhavy, and J. R. Beckwith. 1979. Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm. J. Bacteriol. 139:19-31.
4. Bedouelle, H., P. J. Bassford, Jr., A. V. Fowler, I. Zabin, J. Beckwith, and M. Hofnung. 1980. Mutations which alter the function of the signal sequence of the maltose binding protein of Escherichia coli. Nature 285:78-81.
5. Benson, S. A., E. Bremer, and T. J. Silhavy. 1984. Intragenic regions required for LamB export. Proc. Natl. Acad. Sci. USA 81:3830-3834.
6. Broome-Smith, J. K., and B. G. Spratt. 1986. A vector for the construction of translational fusions to TEM β-lactamase and the analysis of protein export signals and membrane protein topology. Gene 49:341-349.
7. Casey, W. M., and N. A. Nguyen. 1996. Use of the green fluorescent protein to rapidly assess viability of E. coli in preserved solutions. PDA J. Pharm. Sci. Technol. 50:352-355.
8. Chen, J. C., D. S. Weiss, J. M. Ghigo, and J. Beckwith. 1999. Septal localization of FtsQ, an essential cell division protein in Escherichia coli. J. Bacteriol. 181:521-530.
9. Crameri, A., E. A. Whitehorn, E. Tate, and W. P. C. Stemmer. 1996. Improved green fluorescent protein by molecular evolution using DNA shuffling. Nat. Biotechnol. 14:315-319.
10. Emr, S. D., and P. J. Bassford, Jr. 1982. Localization and processing of outer membrane and periplasmic proteins in Escherichia coli strains harboring export-specific suppressor mutations. J. Biol. Chem. 257:5852-5860.
11. Emr, S. D., J. Hedgpeth, J. M. Clement, T. J. Silhavy, and M. Hofnung. 1980. Sequence analysis of mutations that prevent export of lambda receptor, an Escherichia coli outer membrane protein. Nature 285:82-85.
12. Emr, S. D., M. Schwartz, and T. J. Silhavy. 1978. Mutations altering the cellular localization of the phage lambda receptor, an Escherichia coli outer membrane protein. Proc. Natl. Acad. Sci. USA 75:5802-5806.
13. Emr, S. D., and T. J. Silhavy. 1980. Mutations affecting localization of an Escherichia coli outer membrane protein, the bacteriophage lambda receptor. J. Mol. Biol. 141:63-90.
14. Fikes, J. D., and P. J. Bassford, Jr. 1987. Export of unprocessed precursor maltose-binding protein to the periplasm of Escherichia coli cells. J. Bacteriol. 169:2352-2359.
15. Froshauer, S., G. N. Green, D. Boyd, K. McGovern, and J. Beckwith. 1988. Genetic analysis of the membrane insertion and topology of Ma1F, a cytoplasmic membrane protein of Escherichia coli. J. Mol. Biol. 200:501-511.
16. Gannon, P. M., and C. A. Kumamoto. 1993. Mutations of the molecular chaperone protein SecB, which alter the interaction between SecB and maltose-binding protein. J. Biol. Chem. 268:1590-1595.
17. Gardel, C., S. Benson, J. Hunt, S. Michaelis, and J. Beckwith. 1987. secD, a new gene involved in protein export in Escherichia coli. J. Bacteriol. 169: 1286-1290.
18. Georgiou, C. D., T. J. Dueweke, and R. B. Gennis. 1988. β-Galactosidase gene fusions as probes for the cytoplasmic regions of subunits I and II of the membrane-bound cytochrome d terminal oxidase from Escherichia coli. J. Biol. Chem. 263:13130-13137.
19. Ghigo, J. M., D. S. Weiss, J. C. Chen, J. C. Yarrow, and J. Beckwith. 1999. Localization of FtsL to the Escherichia coli septal ring. Mol. Microbiol. 31:725-737.
20. Giorgi, F. D., M. Brini, C. Bastianutto, R. Marsault, M. Montero, P. Pizzo, R. Rosi, and R. Rizzuto. 1996. Targeting aequorin and green fluorescent protein to intracellular organelles. Gene 173:113-117.
21. Hall, M. N., M. Schwartz, and T. J. Silhavy. 1982. Sequence information within the lamB genes is required for proper routing of the bacteriophage lambda receptor protein to the outer membrane of Escherichia coli K-12. J. Mol. Biol. 156:93-112.
22. Hampton, R. Y., A. Koning, R. Wright, and J. Rine. 1996. In vivo examination of membrane protein localization and degradation with the green fluorescent protein. Proc. Natl. Acad. Sci. USA 93:828-833.
23. Harlow, E., and D. Lane. 1988. Antibodies: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
24. Hoffman, C. S., and A. Wright. 1985. Fusions of secreted proteins to alkaline phosphatase: an approach for studying protein secretion. Proc. Natl. Acad. Sci. USA 82:5107-5111.
25. Inoue, H., H. Nojima, and H. Okayama. 1990. High efficiency transformation of Escherichia coli with plasmids. Gene 96:23-28.
26. Ito, K., T. Sato, and T. Yura. 1977. Synthesis and assembly of the membrane proteins in E. coli. Cell 11:551-559.
27. Izard, J. W., and D. A. Kendall. 1994. Signal peptides: exquisitely designed transport promoters. Mol. Microbiol. 13:765-773.
28. Kim, S. K., and J. C. Wang. 1998. Localization of F plasmid SopB protein to positions near the poles of Escherichia coli cells. Proc. Natl. Acad. Sci. USA 95:1523-1527.
29. Kreusch, A., A. Neubuser, E. Schiltz, J. Weckesser, and G. E. Schulz. 1994. Structure of the membrane channel porin from Rhodopseudomonas blastica at 2.0 Å resolution. Protein Sci. 3:58-63.
30. Kumamoto, C. A., and J. Beckwith. 1983. Mutations in a new gene, secB, cause defective protein localization in Escherichia coli. J. Bacteriol. 154:253-260.
31. Kumamoto, C. A., and J. Beckwith. 1985. Evidence for specificity at an early step in protein export in Escherichia coli. J. Bacteriol. 163:267-274.
32. Lee, C., P. Li, H. Inouye, E. R. Brickman, and J. Beckwith. 1989. Genetic studies on the inability of β-galactosidase to be translocated across the Escherichia coli cytoplasmic membrane. J. Bacteriol. 171:4509-4616.
33. Leff, L. G., and A. A. Leff. 1996. Use of green fluorescent protein to monitor survival of genetically engineered bacteria in aquatic environments. Appl. Environ. Microbiol. 62:3486-3468.
34. Ma, X., D. W. Ehhardt, and W. Margolin. 1996. Colocalization of cell division proteins FtsZ and FtsA to cytoskeletal structures in living Escherichia coli cells by using green fluorescent protein. Proc. Natl. Acad. Sci. USA 93:12998-13003.
35. Ma, X., Q. Sun, R. Wang, G. Singh, E. L. Jonietz, and W. Margolin. 1997. Interactions between heterologous FtsA and FtsZ proteins at the FtsZ ring. J. Bacteriol. 179:6788-6797.
36. Manoil, C. 1990. Analysis of protein localization by use of gene fusions with complementary properties. J. Bacteriol. 172:1035-1042.
37. Manoil, C., and J. Beckwith. 1985. TnphoA: a transposon probe for protein export signals. Proc. Natl. Acad. Sci. USA 82:8129-8133.
38. Manoil, C., and J. Beckwith. 1986. A genetic approach to analyzing membrane protein topology. Science 233:1403-1408.
39. Misteli, T., and D. L. Spector. 1997. Application of the green fluorescent protein in cell biology and biotechnology. Nat. Biotechnol. 15:961-964.
40. Oliver, D. B., and J. Beckwith. 1981. E. coli mutant pleiotropically defective in the export of secreted proteins. Cell 25:765-772.
41. Ormo, M. B., A. Cubitt, K. Kallio, L. A. Gross, R. Y. Tsien, and S. J. Remington. 1996. Crystal structure of the Aequorea victoria green fluorescent protein. Science 273:1392-1395.
42. Raskin, D. M., and P. A. de Boer. 1997. The MinE ring: an FtsZ-independent cell structure required for selection of the correct division site in E. coli. Cell 91:685-694.
43. Rizzuto, R., M. Brini, P. Pizzo, M. Murgia, and T. Pozzan. 1995. Chimeric green fluorescent protein: a new tool for visualizing subcellular organelles in living cells. Curr. Biol. 5:635-642.
44. Shiba, K., K. Ito, T. Yura, and D. P. Cerretti. 1984. A defined mutation in the protein export gene within the spc ribosomal protein operon of Escherichia coli: isolation and characterization of a new temperature-sensitive secY mutant. EMBO J. 3:631-635.
45. Siegele, D. A., and J. C. Hu. 1997. Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations. Proc. Natl. Acad. Sci. USA 94:8168-8172.
46. Silhavy, T. J., M. L. Berman, and L. W. Enquist. 1984. Experiments with gene fusions. Cold Spring Harbor Press, Cold Spring Harbor, N.Y.
47. Snyder, W. B., and T. J. Silhavy. 1995. β-Galactosidase is inactivated by intermolecular disulfide bonds and is toxic when secreted to the periplasm of Escherichia coli. J. Bacteriol. 177:953-963.
48. Sun, Q., and W. Margolin. 1998. FtsZ dynamics during the division cycle of live Escherichia coli cells. J. Bacteriol. 180:2050-2056.
49. Valdivia, R. H., and S. Falkow. 1996. Bacterial genetics by flow cytometry: rapid isolation of Salmonella typhimurium acid-inducible promoters by differential fluorescence induction. Mol. Microbiol. 22:367-378.
50. Vogel, H. J., and D. M. Bonner. 1955. Acetylorornithinase of Escherichia coli: partial purification and some properties. J. Biol. Chem. 218:97-106.
51. Ward, W. W., and S. H. Bokman. 1982. Reversible denaturation of Aequorea green-fluorescent protein: physical separation and characterization of the renatured protein. Biochemistry 21:4535-4540.
52. Weiss, D. S., J. C. Chen, J. M. Ghigo, D. Boyd, and J. Beckwith. 1999. Localization of FtsI (PBP3) to the septal ring requires its membrane anchor, the Z ring, FtsA, FtsQ, and FtsL. J. Bacteriol. 181:508-520.
53. Weiss, D. S., K. Pogliano, M. Carson, L. M. Guzman, C Fraipont, M. Nguyen-Disteche, R. Losick, and J. Beckwith. 1997. Localization of the Escherichia coli cell division protein FtsI (PBP3) to the division site and cell pole. Mol. Microbiol. 25:671-681.
54. Yang, F., L. G. Moss, and G. N. Phillips, Jr. 1996. The molecular structure of green fluorescent protein. Nat. Biotechnol. 14:1246-1251.
55. 2+-mediated GTP-dependent dynamic assembly of bacterial cell division protein FtsZ into asters and polymer networks in vitro. EMBO J. 16:5455-5463.
56. Yu, X. C., A. H. Tran, Q. Sun, and W. Margolin. 1998. Localization of cell division protein FtsK to the Escherichia coli septum and identification of a potential N-terminal targeting domain. J. Bacteriol. 180:1296-1304.