Refractive index-based determination of detergent concentration and its application to the study of membrane proteins

Protein Science

Volumn 14, Issue 8, 2005, Pages 2207-2211

  Strop, Pavel ^b   Brunger, Axel T ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b NONE

Author keywords

Detergent concentration; dn dc; Micelle size; Refractive index

Indexed keywords

DETERGENT; MEMBRANE PROTEIN;

ANALYTIC METHOD; ARTICLE; CORRELATION ANALYSIS; GEL PERMEATION CHROMATOGRAPHY; LIGHT SCATTERING; MICELLE; MOLECULAR WEIGHT; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REFRACTION INDEX;

CHROMATOGRAPHY, GEL; DETERGENTS; MEMBRANE PROTEINS; MICELLES; REFERENCE STANDARDS; REFRACTOMETRY;

EID: 23644444909     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.051543805     Document Type: Article

References (19)

1. Corti, M., Minero, C., and Degiorgio, V. 1984. Cloud point transition in nonionic micellar solutions. J. Phys. Chem. 88: 309.
2. daCosta, C.J. and Baenziger, J.E. 2003. A rapid method for assessing lipid:protein and detergent:protein ratios in membrane-protein crystallization. Acta Crystallogr. D Biol. Crystallogr. 59: 77-83.
3. Eriks, L.R., Mayor, J.A., and Kaplan, R.S. 2003. A strategy for identification and quantification of detergents frequently used in the purification of membrane proteins. Anal. Biochem. 323: 234-241.
4. Fisher, L.E., Engelman, D.M., and Sturgis, J.N. 1999. Detergents modulate dimerization, but not helicity, of the glycophorin A transmembrane domain. J. Mol. Biol. 293: 639-651.
5. Fisher, L.E., Engelman, D.M., and Sturgis, J.N. 2003. Effect of detergents on the association of the glycophorin a transmembrane helix. Biophys. J. 85: 3097-3105.
6. Folta-Stogniew, E. and Williams, K.R. 1999. Implementation of an HPLC size exclusion chromatography and laser light scattering service in a core laboratory. J. Biomol. Tech. 10: 51-63.
7. Hayashi, Y., Matsui, H., and Takagi, T. 1989. Membrane protein molecular weight determined by low-angle laser light-scattering photometry coupled with high-performance gel chromatography. Methods Enzymol. 172: 514-528.
8. Herrmann, K.W. 1962. Non-ionic-cationic micellar properties of dimethyldodecylamine oxide. J. Phys. Chem. 66: 295-300.
9. Knobloch, J.E. and Shaklee, P.N. 1997. Absolute molecular weight distribution of low-molecular-weight heparins by size-exclusion chromatography with multiangle laser light scattering detection. Anal. Biochem. 245: 231-241.
10. Krogh, A., Larsson, B., von Heijne, G., and Sonnhammer, E.L. 2001. Predicting transmembrane protein topology with a hidden Markov model: Application to complete genomes. J. Mol. Biol. 305: 567-580.
11. Lide, D.R. 1994. Handbook of chemistry and physics, 74th ed. CRC Press, Boca Raton, LA.
12. Ma, P. and Zemmel, R. 2002. Value of novelty? Nat. Rev. Drug Discov. 1: 571-572.
13. Urbani, A. and Warne, T. 2005. A colorimetric determination for glycosidic and bile salt-based detergents: Applications in membrane protein research. Anal. Biochem. 336: 117-124.
14. VanAken, T., Foxall-VanAken, S., Castleman, S., and Ferguson-Miller, S. 1986. Alkyl glycoside detergents: Synthesis and applications to the study of membrane proteins. Methods Enzymol. 125: 27-35.
15. Wallin, E. and von Heijne, G. 1998. Genome-wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organisms. Protein Sci. 7: 1029-1038.
16. Wen, J., Arakawa, T., and Philo, J.S. 1996. Size-exclusion chromatography with on-line light-scattering, absorbance, and refractive index detectors for studying proteins and their interactions. Anal. Biochem. 240: 155-166.
17. Wiener, M.C. 2004. A pedestrian guide to membrane protein crystallization. Methods 34: 364-372.
18. Wyatt, P.J. 1993. Light scattering and the absolute characterization of macromolecules. Analytica Chimica Acta 272: 1-40.
19. Yernool, D., Boudker, O., Folta-Stogniew, E., and Gouaux, E. 2003. Trimeric subunit stoichiometry of the glutamate transporters from Bacillus caldotenax and Bacillus stearothermophilus. Biochemistry 42: 12981-12988.