Detection of soluble co-factor dependent protein expression in vivo: Application to the 4'-phosphopantetheinyl transferase PptT from Mycobacterium tuberculosis

Journal of Structural Biology

Volumn 183, Issue 3, 2013, Pages 320-328

  Rottier, Karine ^a,b   Faille, Alexandre ^a,b   Prudhomme, Thomas ^a,b   Leblanc, Cécile ^a,b   Chalut, Christian ^a,b   Cabantous, Stéphanie ^c,d,e   Guilhot, Christophe ^a,b   Mourey, Lionel ^a,b   Pedelacq, Jean Denis ^a,b  

^a INSTITUT DE PHARMACOLOGIE ET DE BIOLOGIE STRUCTURALE   (France)

^b UNIVERSITÉ PAUL SABATIER   (France)

^c CANCER RESEARCH CENTER OF TOULOUSE   (France)

^d UNIVERSITÉ DE TOULOUSE   (France)

^e INSTITUT CLAUDIUS REGAUD   (France)

Author keywords

4' Phosphopantetheinyl transferase; Co factor; Domain trapping; Polyketide; Split GFP; Tuberculosis

Indexed keywords

4' PHOSPHOPANTETHEINYL TRANSFERASE; AMINO ACID; COENZYME A; POLYKETIDE SYNTHASE; TRANSFERASE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; GENETIC SELECTION; IN VITRO STUDY; IN VIVO STUDY; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PATHOGENICITY; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN EXPRESSION; SOLUBILITY;

ESCHERICHIA COLI; MYCOBACTERIUM TUBERCULOSIS;

4′-PHOSPHOPANTETHEINE; 4′-PHOSPHOPANTETHEINYL TRANSFERASE; ACP; ACPS; ACYL CARRIER PROTEIN; ACYL CARRIER PROTEIN SYNTHASE; ANHYDROTETRACYCLINE; ANTET; BOVINE SERUM ALBUMIN; BSA; CO-FACTOR; COA; COENZYME A; DHFR; DIFFERENTIAL SCANNING FLUORIMETRY; DIHYDROFOLATE REDUCTASE; DITHIOTHREITOL; DOMAIN TRAPPING; DSF; DTT; GFP; GREEN FLUORESCENT PROTEIN; IMAC; IMMOBILIZED METAL ION AFFINITY CHROMATOGRAPHY; IPTG; ISOPROPYL Β-D-1-THIOGALACTOPYRANOSIDE; KAN; KANAMYCIN; LB; LURIA-BERTANI; MALTOSE BINDING PROTEIN; MBP; P-PANT; PCR; PDIM; PHTIOCEROL DIMYCOCEROSATE; PKS; POLYKETIDE; POLYKETIDE SYNTHASE; POLYMERASE CHAIN REACTION; SDS-PAGE; SEC; SFP; SIZE-EXCLUSION CHROMATOGRAPHY; SMALL UBIQUITIN-RELATED MODIFIER; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; SPEC; SPECTINOMYCIN; SPLIT-GFP; SUMO; SURFACTIN SYNTHETASE-ACTIVATING ENZYME; TUBERCULOSIS;

ACYL CARRIER PROTEIN; BACTERIAL PROTEINS; CLONING, MOLECULAR; COENZYME A; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; GREEN FLUORESCENT PROTEINS; MAGNESIUM; MYCOBACTERIUM TUBERCULOSIS; PROTEIN FOLDING; RECOMBINANT FUSION PROTEINS; SOLUBILITY; TRANSFERASES (OTHER SUBSTITUTED PHOSPHATE GROUPS);

EID: 84883461648     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2013.07.010     Document Type: Article

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