Crystal structures of substrate binding to Bacillus subtilis holo-(acyl carrier protein) synthase reveal a novel trimeric arrangement of molecules resulting in three active sites

Structure

Volumn 8, Issue 8, 2000, Pages 883-895

  Parris, Kevin D ^a   Lin, Laura ^a   Tam, Amy ^a   Mathew, Rebecca ^a   Hixon, Jeffrey ^b   Stahl, Mark ^a   Fritz, Christian C ^b   Seehra, Jasbir ^a   Somers, William S ^a  

^a PFIZER INC   (United States)

^b MILLENNIUM PHARMACEUTICALS INC   (United States)

Author keywords

Acyl carrier protein; Coenzyme A; Fatty acid biosynthesis; Phosphopantetheinyl transferase; Three dimensional structure; X ray crystallography

Indexed keywords

ACYL CARRIER PROTEIN; ACYL CARRIER PROTEIN SYNTHETASE; CARRIER PROTEIN; COENZYME A; TRANSFERASE; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS SUBTILIS; CATALYSIS; COMPLEX FORMATION; CRYSTAL STRUCTURE; DIMERIZATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME CONFORMATION; FATTY ACID SYNTHESIS; HYDROPHOBICITY; NONHUMAN; PRIORITY JOURNAL; STRUCTURE ANALYSIS;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); POSIBACTERIA;

EID: 0034662753     PISSN: 09692126     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0969-2126(00)00178-7     Document Type: Article

References (42)

1. Regulation of fatty acid biosynthesis in Escherichia coli
2. On the structure of fatty acid synthetase of yeast
3. Fatty acid synthesis and its regulation
4. Purification and characterization of the acyl carrier protein of the Streptomyces glaucescens tetracenomycin C polyketide synthase
5. Molecular genetics of polyketides and its comparison to fatty acid biosynthesis
6. A nonribosomal system of peptide biosynthesis
7. Multidomain enzymes involved in peptide synthesis
8. 3]acetate
9. 13C-labeling studies
10. Escherichia coli acyl carrier protein
11. Overexpression, purification, and characterization of Escherichia coli acyl carrier protein and two mutant proteins
12. Improved purification of acyl carrier protein
13. Three-dimensional structure of acyl carrier protein in solution determined by nuclear magnetic resonance and the combined use of dynamical simulated annealing and distance geometry
14. Thiolactomycin resistance in Escherichia coli is associated with the multidrug resistance efflux pump encoded by emrAB
15. Protein EnvM is the NADH-dependent enoyl-ACP Reductase (Fabl) of Escherichia coli
16. inhA, a gene encoding a target for isoniazid and ethionamide in Mycobacterium tuberculosis
17. Crystal structure and function of the isoniazid target of Mycobacterium tuberculosis
18. Enzymatic characterization of the target for isoniazid in Mycobacterium tuberculosis
19. A new enzyme superfamily - The phosphopantetheinyl transferases
20. Acyl carrier protein X, acyl carrier protein synthetase
21. Cloning, overproduction, and characterization of the Escherichia coli holo-acyl carrier protein synthase
22. Crystal structure of the surfactin synthetase-activating enzyme Sfp: A prototype of the 4'-phosphopantetheinyl transferase superfamily
23. Determination of macromolecular structures from anomalous diffraction of synchrotron radiation
24. AMoRe: An automated package for molecular replacement
25. Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side-chain length of the penultimate amino acid
26. Protein structure comparison by alignment of distance matrices
27. Crystal structure of the histone acetyltransferase domain of the human PCAF transcriptional regulator bound to coenzyme A
28. Holo-[acyl-carrier-protein] synthase of Escherichia coli
29. Holo-(acyl carrier protein) synthase and phosphopantetheinyl transfer in Escherichia coli
30. Crystal structure of the zinc-dependent β-lactamase from Bacillus cereus at 1.9 Å resolution: Binuclear active site with features of a mononuclear enzyme
31. 2+ complex and mechanism of its pyrophosphohydrolase action
32. A mutant of Escherichia coli conditionally defective in the synthesis of holo-[acyl carrier protein]
33. Serine residue 45 of nodulation protein NodF from Rhizobium leguminosarum bv. viciae is essential for its biological function
34. The D-alanyl carrier protein in Lactobacillus casei: Cloning, sequencing, and expression of dltC
35. Isolation and properties of acyl carrier protein phosphodiesterase of Escherichia coli
36. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
37. Processing of X-ray diffraction data collected in oscillation mode
38. Automated structure solution for MIR and MAD
39. Maximum-likelihood heavy-atom parameter refinement in the MIR and MAD methods
40. Crystallography and NMR system: A new software suite for macromolecular structure determination
41. Ribbons
42. Protein folding and association: Insights from the interfacial and thermodynamic properties of hydrocarbons