Structure-Based Mutational Analysis of the 4′-Phosphopantetheinyl Transferases Sfp from Bacillus subtilis: Carrier Protein Recognition and Reaction Mechanism

Biochemistry

Volumn 43, Issue 14, 2004, Pages 4128-4136

  Mofid, Mohammad Reza ^a   Finking, Robert ^a   Essen, Lars Oliver ^a   Marahiel, Mohamed A ^a  

^a PHILIPPS UNIVERSITY MARBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMICAL BONDS; CRYSTAL STRUCTURE; FATTY ACIDS; HYDROXYLATION; METABOLISM; POLYMERIZATION; REACTION KINETICS;

FATTY ACID SYNTHASES (FAS); POLYKETIDE SYNTHASES (PKS);

PROTEINS;

4' PHOSPHOPANTETHEINYL TRANSFERASE; ACYL CARRIER PROTEIN; ACYL CARRIER PROTEIN SYNTHETASE; BACTERIAL ENZYME; CARRIER PROTEIN; COENZYME A; FATTY ACID SYNTHASE; HYDROXYL GROUP; MUTANT PROTEIN; PANTETHEINE PHOSPHATE; PEPTIDE SYNTHASE; PHOSPHATE; POLYKETIDE SYNTHASE; PROTEIN SFP; SERINE; SYNTHETASE; TRANSFERASE; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS SUBTILIS; CRYSTAL STRUCTURE; ENZYME BINDING; ENZYME MECHANISM; ENZYME SPECIFICITY; ENZYME STRUCTURE; GENETIC CONSERVATION; MOLECULAR MODEL; MOLECULAR RECOGNITION; MUTATION; NONHUMAN; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN MOTIF; REACTION ANALYSIS;

ACYL CARRIER PROTEIN; BACILLUS SUBTILIS; BACTERIAL PROTEINS; BINDING SITES; CATALYSIS; CATIONS, DIVALENT; COENZYME A; DNA MUTATIONAL ANALYSIS; LIPOPROTEINS; METALS, HEAVY; PEPTIDES, CYCLIC; PROTEIN PROCESSING, POST-TRANSLATIONAL; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; TRANSFERASES (OTHER SUBSTITUTED PHOSPHATE GROUPS);

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); EUKARYOTA; POSIBACTERIA;

EID: 1842531416     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi036013h     Document Type: Article

References (32)

1. Cane, D. E., and Walsh, C. T. (1999) Chem. Biol. 6, 319-325.
2. Hopwood, D. A. (1997) Chem. Rev. 97, 2465-2497.
3. Kleinkauf, H. (2000) Biofactors 11, 91-92.
4. Walsh, C. T., Gehring, A. M., Weinreb, P. H., Luis, E. N., and Flugel, R. S. (1997) Curr. Opin. Chem. Biol. 1, 309-315.
5. Van Wageningen, A., Kirkpatrick, P., Williams, D., Harris, B., Kershaw, J., Lennard, N., Jones, M., Jones, S., and Solenberg, P. (1998) Chem. Biol. 5, 155-162.
6. Weber, G., Schorgendorfer, K., Schneider-Scherzer, E., and Leitner, E. (1994) Curr. Genet. 26, 120-125.
7. Lambalot, R. H., Gehring, A. M., Flugel, R. S., Zuber, P., LaCelle, M., Marahiel, M. A., Reid, R., Khosla, C., and Walsh, C. T. (1996) Chem. Biol. 3, 923-936.
8. Quadri, L. E., Sello, J., Keating, T. A., Weinreb, P. H., and Walsh, C. T. (1998) Chem. Biol. 5, 631-645.
9. Marahiel, M. A., Stachelhaus, T., and Mootz, H. D. (1997) Chem. Rev. 97, 2651-2674.
10. Borchert, S., Stachelhaus, T., and Marahiel, M. A. (1994) J. Bacteriol. 176, 2458-2462.
11. Mootz, H. D., Schörgendorfer, K., and Marahiel, M. A. (2002) FEMS Microbiol. Lett. 213, 51-57.
12. Kiriukhin, M. Y., and Neuhaus, F. C. (2001) J. Bacteriol. 183, 2051-2058.
13. Ritsema, T., Geiger, O., van Dillewijn, P., Lugtenberg, B. J. J., and Spaink, H. P. (1994) J. Bacteriol. 176, 7740-7743.
14. Silakowski, B., Schairer, H. U., Ehret, H., Kunze, B., Weinig, S., Nordsiek, G., Brandt, P., Blocker, H., Hofle, G., Beyer, S., and Muller, R. (1999) J. Biol. Chem. 274, 37391-37399.
15. Fichtlscherer, F., Wellein, C., Mittag, M., and Schweizer, E. (2000) Eur. J. Biochem. 267, 2666-2671.
16. Lambalot, R. H., and Walsh, C. T. (1997) Methods Enzymol. 279, 254-262.
17. Quadri, L. E., Weinreb, P. H., Lei, M., Nakano, M. M., Zuber, P., and Walsh, C. T. (1998) Biochemistry 37, 1585-1595.
18. Finking, R., Solsbacher, J., Konz, D., Schobert, M., Schäfer, A., Jahn, D., and Marahiel, M. A. (2002) J. Biol. Chem. 277, 50293-50302.
19. Reuter, K., Mofid, M. R., Marahiel, M. A., and Ficner, R. (1999) EMBO J. 18, 6823-6831.
20. Parris, K. D., Lin, L., Tam, A., Mathew, R., Hixon, J., Stahl, M., Fritz, C. C., Seehra, J., and Somers, W. S. (2000) Struct. Folding Des. 8, 883-895.
21. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
22. Mofid, M. R., Finking, R., and Marahiel, M. A. (2002) J. Biol. Chem. 277, 17023-17031.
23. Mofid, M. R., Marahiel, M. A., Ficner, R., and Reuter, K. (1999) Acta Crystallogr., Sect. D: Biol. Crystallogr. 55, 1098-1100.
24. Linne, U., Stein, D. B., Mootz, H. D., and Marahiel, M. A. (2003) Biochemistry 42, 5114-5124.
25. Mootz, H. D., Finking, R., and Marahiel, M. A. (2001) J. Biol. Chem. 276, 37289-37298.
26. Chirgadze, N. Y., Briggs, S. L., McAllister, K. A., Fischl, A. S., and Zhao, G. (2000) EMBO J. 19, 5281-5287.
27. Nakano, M. M., Corbell, N., Besson, J., and Zuber, P. (1992) Mol. Gen. Genet. 232, 313-321.
28. Modis, Y., and Wierenga, R. (1998) Structure 6, 1345-1350.
29. Fabiane, S. M., Sohi, M. K., Wan, T., Payne, D. J., Bateson, J. H., Mitchell, T., and Sutton, B. J. (1998) Biochemistry 37, 12404-12411.
30. Lin, J., Abeygunawardana, C., Frick, D. N., Bessman, M. J., and Mildvan, A. S. (1997) Biochemistry 36, 1199-1211.
31. Guo, S., and Bhattacharjee, J. K. (2003) FEMS Microbiol. Lett. 224, 261-267.
32. Jones, T., Zou, J. Y., Cowan, S. W., and Kjeldgaard (1991) Acta Crystallogr. A 47, 110-119.