An efficient and generic strategy for producing soluble human proteins and domains in E. coli by screening construct libraries

Proteins: Structure, Function and Genetics

Volumn 65, Issue 2, 2006, Pages 266-273

  Cornvik, Tobias ^a,b   Dahlroth, Sue Li ^a,b   Magnusdottir, Audur ^a,b   Flodin, Susanne ^b   Engvall, Benita ^b   Lieu, Victoria ^b   Ekberg, Monica ^b   Nordlund, Pär ^b  

^a STOCKHOLM UNIVERSITY   (Sweden)

^b KAROLINSKA INSTITUTE   (Sweden)

Author keywords

Colony screen; Directed evolution; Protein domains; Protein overexpression

Indexed keywords

RECOMBINANT PROTEIN;

AMINO TERMINAL SEQUENCE; ANALYTIC METHOD; ARTICLE; COLONY FILTRATION BLOT; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN OVEREXPRESSION; PROTEIN PURIFICATION; STRUCTURAL GENOMICS; TRANSLATION INITIATION;

CLONING, MOLECULAR; ESCHERICHIA COLI; GENE LIBRARY; HUMANS; RECOMBINANT PROTEINS; SOLUBILITY;

EUKARYOTA; MAMMALIA;

EID: 33748989829     PISSN: 08873585     EISSN: 10970134     Source Type: Journal    
DOI: 10.1002/prot.21090     Document Type: Article

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