Engineering the cellular protein secretory pathway for enhancement of recombinant tissue plasminogen activator expression in Chinese hamster ovary cells: Effects of CERT and XBP1s genes

Journal of Microbiology and Biotechnology

Volumn 23, Issue 8, 2013, Pages 1116-1122

  Rahimpour, Azam ^a   Vaziri, Behrouz ^a   Moazzami, Reza ^a   Nematollahi, Leila ^a   Barkhordari, Farzaneh ^a   Kokabee, Leila ^a   Adeli, Ahmad ^a   Mahboudi, Fereidoun ^a  

^a PASTEUR INSTITUTE OF IRAN   (Iran)

Author keywords

Cell line engineering; Ceramide transfer protein; Chinese hamster ovary cells; Tissue plasminogen activator; X box binding protein 1

Indexed keywords

ALTEPLASE; MESSENGER RNA; X BOX BINDING PROTEIN 1;

ANIMAL CELL; ARTICLE; CELL ENGINEERING; CERAMIDE TRANSFER PROTEIN GENE; CHO CELL; CONTROLLED STUDY; GENE OVEREXPRESSION; HETEROLOGOUS EXPRESSION; NONHUMAN; PROTEIN EXPRESSION; PROTEIN SECRETION; WESTERN BLOTTING; X BOX BINDING PROTEIN 1 GENE;

ANIMALS; CARRIER PROTEINS; CHO CELLS; CRICETINAE; CRICETULUS; DNA-BINDING PROTEINS; FEMALE; GENE EXPRESSION; METABOLIC ENGINEERING; RECOMBINANT PROTEINS; SECRETORY PATHWAY; TISSUE PLASMINOGEN ACTIVATOR;

EID: 84883037397     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.1302.02035     Document Type: Article

References (36)

1. Bard F, Malhotra V. 2006. The formation of TGN-to-plasmamembrane transport carriers. Annu. Rev. Cell Dev. Biol. 22: 439-455.
2. Becker E, Florin L, Pfizenmaier K, Kaufmann H. 2008. An XBP-1 dependent bottle-neck in production of IgG subtype antibodies in chemically defined serum-free Chinese hamster ovary (CHO) fed-batch processes. J. Biotechnol. 135: 217-223.
3. Borth N, Mattanovich D, Kunert R, Katinger H. 2005. Effect of increased expression of protein disulfide isomerase and heavy chain binding protein on antibody secretion in a recombinant CHO cell line. Biotechnol. Prog. 21: 106-111.
4. Butler M. 2005. Animal cell cultures: recent achievements and perspectives in the production of biopharmaceuticals. Appl. Microbiol. Biotechnol. 68: 283-291.
5. Cacciatore JJ, Chasin LA, Leonard EF. 2010. Gene amplification and vector engineering to achieve rapid and high-level therapeutic protein production using the Dhfr-based CHO cell selection system. Biotechnol. Adv. 28: 673-681.
6. Figueroa Jr B, Chen S, Oyler GA, Hardwick JM, Betenbaugh MJ. 2004. Aven and Bcl-xL enhance protection against apoptosis for mammalian cells exposed to various culture conditions. Biotechnol. Bioeng. 85: 589-600.
7. Florin L, Pegel A, Becker E, Hausser A, Olayioye MA, Kaufmann H. 2009. Heterologous expression of the lipid transfer protein CERT increases therapeutic protein productivity of mammalian cells. J. Biotechnol. 141: 84-90.
8. Fugmann T, Hausser A, Schoffler P, Schmid S, Pfizenmaier K, Olayioye MA. 2007. Regulation of secretory transport by protein kinase D-mediated phosphorylation of the ceramide transfer protein. J. Cell Biol. 178: 15-22.
9. Fussenegger M, Schlatter S, Datwyler D, Mazur X, Bailey JE. 1998. Controlled proliferation by multigene metabolic engineering enhances the productivity of Chinese hamster ovary cells. Nat. Biotechnol. 16: 468-472.
10. Geserick C, Bonarius HP, Kongerslev L, Hauser H, Mueller PP. 2000. Enhanced productivity during controlled proliferation of BHK cells in continuously perfused bioreactors. Biotechnol. Bioeng. 69: 266-274.
11. Hanada K, Kumagai K, Yasuda S, Miura Y, Kawano M, Fukasawa M, Nishijima M. 2003. Molecular machinery for non-vesicular trafficking of ceramide. Nature 426: 803-809.
12. Jaluria P, Chu C, Betenbaugh M, Shiloach J. 2008. Cells by design: a mini-review of targeting cell engineering using DNA microarrays. Mol. Biotechnol. 39: 105-111.
13. Jayapal KP, Wlaschin KF, Hu WS, Yap MGS. 2007. Recombinant protein therapeutics from CHO cells - 20 years and counting. Chem. Eng. Prog. 103: 40-47.
14. Josse L, Smales CM, Tuite MF. 2012. Engineering the chaperone network of CHO cells for optimal recombinant protein production and authenticity. Methods Mol. Biol. 824: 595-608.
15. Khan SU, Schroder M. 2008. Engineering of chaperone systems and of the unfolded protein response. Cytotechnology 57: 207-231.
16. Kim JY, Kim YG, Lee GM. 2012. CHO cells in biotechnology for production of recombinant proteins: current state and further potential. Appl. Microbiol. Biotechnol. 93: 917-930.
17. Kim SH, Lee GM. 2007. Functional expression of human pyruvate carboxylase for reduced lactic acid formation of Chinese hamster ovary cells (DG44). Appl. Microbiol. Biotechnol. 76: 659-665.
18. Kramer O, Klausing S, Noll T. 2010. Methods in mammalian cell line engineering: from random mutagenesis to sequencespecific approaches. Appl. Microbiol. Biotechnol. 88: 425-436.
19. Ku SC, Ng DT, Yap MG, Chao SH. 2008. Effects of overexpression of X-box binding protein 1 on recombinant protein production in Chinese hamster ovary and NS0 myeloma cells. Biotechnol. Bioeng. 99: 155-164.
20. Ku SC, Toh PC, Lee YY, Chusainow J, Yap MG, Chao SH. 2010. Regulation of XBP-1 signaling during transient and stable recombinant protein production in CHO cells. Biotechnol. Prog. 26: 517-526.
21. Lee AH, Iwakoshi NN, Glimcher LH. 2003. XBP-1 regulates a subset of endoplasmic reticulum resident chaperone genes in the unfolded protein response. Mol. Cell Biol. 23: 7448-7459.
22. Meents H, Enenkel B, Werner RG, Fussenegger M. 2002. p27Kip1-mediated controlled proliferation technology increases constitutive sICAM production in CHO-DUKX adapted for growth in suspension and serum-free media. Biotechnol. Bioeng. 79: 619-627.
23. O'Callaghan PM, James DC. 2008. Systems biotechnology of mammalian cell factories. Brief. Funct. Genomic. Proteomic. 7: 95-110.
24. Ohya T, Hayashi T, Kiyama E, Nishii H, Miki H, Kobayashi K, et al. 2008. Improved production of recombinant human antithrombin III in Chinese hamster ovary cells by ATF4 overexpression. Biotechnol. Bioeng. 100: 317-324.
25. Onitsuka M, Kim WD, Ozaki H, Kawaguchi A, Honda K, Kajiura H, et al. 2012. Enhancement of sialylation on humanized IgG-like bispecific antibody by overexpression of alpha2,6-sialyltransferase derived from Chinese hamster ovary cells. Appl. Microbiol. Biotechnol. 94: 69-80.
26. Park H, Kim IH, Kim IY, Kim KH, Kim HJ. 2000. Expression of carbamoyl phosphate synthetase I and ornithine transcarbamoylase genes in Chinese hamster ovary DHFR-cells decreases accumulation of ammonium ion in culture media. J. Biotechnol. 81: 129-140.
27. Peng RW, Fussenegger M. 2009. Molecular engineering of exocytic vesicle traffic enhances the productivity of Chinese hamster ovary cells. Biotechnol. Bioeng. 102: 1170-1181.
28. Rouf SA, Moo-Young M, Chisti Y. 1996. Tissue-type plasminogen activator: characteristics, applications and production technology. Biotechnol. Adv. 14: 239-266.
29. Schroder, M. 2006. The unfolded protein response. Mol. Biotechnol. 34: 279-290.
30. Seth G, Charaniya S, Wlaschin KF, Hu WS. 2007. In pursuit of a super producer - alternative paths to high producing recombinant mammalian cells. Curr. Opin. Biotechnol. 18: 557-564.
31. Tey BT, Singh RP, Piredda L, Piacentini M, Al-Rubeai M. 2000. Bcl-2 mediated suppression of apoptosis in myeloma NS0 cultures. J. Biotechnol. 79: 147-159.
32. Tigges M, Fussenegger M. 2006. Xbp1-based engineering of secretory capacity enhances the productivity of Chinese hamster ovary cells. Metab. Eng. 8: 264-272.
33. Wong NS, Yap MG, Wang DI. 2006. Enhancing recombinant glycoprotein sialylation through CMP-sialic acid transporter over expression in Chinese hamster ovary cells. Biotechnol. Bioeng. 93: 1005-1016.
34. Wurm FM. 2004. Production of recombinant protein therapeutics in cultivated mammalian cells. Nat. Biotechnol. 22: 1393-1398.
35. Zhu J. 2012. Mammalian cell protein expression for biopharmaceutical production. Biotechnol. Adv. 25: 1158-1170.
36. Zustiak MP, Dorai H, Betenbaugh MJ, Sauerwald TM. 2012. Controlling apoptosis to optimize yields of proteins from mammalian cells. Methods Mol. Biol. 801: 111-123.