DNase I and Proteinase K eliminate DNA from injured or dead bacteria but not from living bacteria in microbial reference systems and natural drinking water biofilms for subsequent molecular biology analyses

Journal of Microbiological Methods

Volumn 94, Issue 3, 2013, Pages 161-169

  Villarreal, Jessica Varela ^a   Jungfer, Christina ^a   Obst, Ursula ^a   Schwartz, Thomas ^a  

^a KARLSRUHE INSTITUTE OF TECHNOLOGY   (Germany)

Author keywords

DNase; Drinking water biofilms; Live dead discrimination; PCR based analyses; Reference bacteria

Indexed keywords

DEOXYRIBONUCLEASE I; DRINKING WATER; GENOMIC DNA; PROTEINASE K; RNA 16S;

ARTICLE; BACTERIAL CELL; BACTERIAL COUNT; BACTERIUM CULTURE; BACTERIUM DETECTION; BIOFILM; CELL COUNT; CELL DAMAGE; CELL DEATH; CELL MEMBRANE; CONTROLLED STUDY; DENATURING GRADIENT GEL ELECTROPHORESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; SALMONELLA ENTERICA; STAPHYLOCOCCUS AUREUS; WATER ANALYSIS;

DNASE; DRINKING WATER BIOFILMS; LIVE/DEAD DISCRIMINATION; PCR BASED ANALYSES; REFERENCE BACTERIA;

BACTERIA; BIOFILMS; DEOXYRIBONUCLEASE I; DNA, BACTERIAL; DRINKING WATER; ENDOPEPTIDASE K; MICROBIAL VIABILITY; POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; WATER MICROBIOLOGY;

EID: 84880383091     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2013.06.009     Document Type: Article

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