Direct estimate of active bacteria: CTC use and limitations

Journal of Microbiological Methods

Volumn 52, Issue 1, 2003, Pages 19-28

  Créach, Véronique ^a,b   Baudoux, Anne Claire ^a   Bertru, Georges ^a   Rouzic, Bertrand Le ^a  

^a UNIVERSITÉ DE RENNES 1   (France)

^b NETHERLANDS INSTITUTE OF ECOLOGY NIOO KNAW   (Netherlands)

Author keywords

5 Cyano 2,3 ditolyl tetrazolium chloride (CTC); Active bacteria; Method

Indexed keywords

5 CYANO 2,3 DITOLYLTETRAZOLIUM CHLORIDE; CARBON DIOXIDE; TETRAZOLIUM; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL COUNT; BACTERIAL GROWTH; BACTERIUM CULTURE; CELL VIABILITY; COLONY FORMING UNIT; CONTROLLED STUDY; CORRELATION ANALYSIS; ESCHERICHIA COLI; EVOLUTION; GROWTH CURVE; MICROBIAL ACTIVITY; MICROBIAL RESPIRATION; NONHUMAN; PRIORITY JOURNAL; STANDARDIZATION;

BACTERIOLOGICAL TECHNIQUES; COLONY COUNT, MICROBIAL; ESCHERICHIA COLI; TETRAZOLIUM SALTS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0037208471     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(02)00128-8     Document Type: Article

References (40)

1. Bartscht K., Cynpionka H., Overmann J. Evaluation of the cell activity and methods for the cultivation of bacteria from natural lake. FEMS Microbiol. Ecol. 28:1999;249-259.
2. Berman T., Kaplan B., Chav S., Viner Y., Sherr B.F., Sherr E.B. Metabolically active bacteria in lake Kinneret. Aquat. Microb. Ecol. 23:2001;213-224.
3. Bhupathiraju V.K., Hernandez M., Landfear D., Alvarez-Cohen L. Application of a tetrazolium dye as an indicator of viability in anaerobic bacteria. J. Microbiol. Methods. 37:1999;231-243.
4. Boulos L., Prevost M., Barbeau B., Coallier J., Desjardin R. LIVE/DEAD® BacLight™: application of a new, rapid staining method for direct enumeration of viable and total bacteria in drinking water. J. Microbiol. Methods. 37:1999;77-86.
5. Button D.K., Schut F., Quang P., Martin R., Robertson B.R. Viability and isolation of marine bacteria by dilution culture: theory, procedures, and initial results. Appl. Environ. Microbiol. 59:1993;881-891.
6. Caro A., Got P., Baleux B. Physiological changes of Salmonella typhimurium cells under osmotic and starvation conditions by image analysis. FEMS Microbiol. Lett. 179:1999;265-273.
7. Choi J.W., Sherr B.F., Sherr E.B. Relation between presence-absence of a visible nucleiod and metabolic activity in bacterioplankton cells. Limnol. Oceanogr. 41:1996;1161-1168.
8. Choi J.W., Sherr B.F., Sherr E.B. Dead or alive? A large fraction of ETS-inactive marine bacterioplankton cells, as assessed by reduction of CTC, can become ETS-active with incubation and substrate addition. Aquat. Microb. Ecol. 18:1999;105-115.
9. 2 production in mixed microbial communities. Microb. Ecol. 34:1997;237-247.
10. del Giorgo P.A., Scarborough G. Increase in the proportion of metabolically active bacteria along gradients of enrichment in freshwater and marine plankton: implications for estimates of bacterial growth and production rates. J. Plankton Res. 17:1995;1905-1924.
11. del Giorgio P.A., Prairie Y.T., Bird D.F. Coupling between rates of bacterial production and the abundance of metabolically active bacteria in lakes, enumerated using CTC reduction and flow cytometry. Microb. Ecol. 34:1997;144-154.
12. Dufour P., Colon M. The tetrazolium reduction method for assessing the viability of individual bacterial cells in aquatic environments: improvements, performance and application. Hydrobiologia. 232:1992;211-218.
13. Epstein S.S., Rossel J. Methodology of in situ grazing experiments: evaluation of a new vital dye for preparation of fluorescently-labelled bacteria. Mar. Ecol., Progr. Ser. 128:1995;143-150.
14. Heijnen C.E., Page S., Van Elsas J.D. Metabolic activity of Flavobacterium strain P25 during starvation and after introduction into bulk soil and rhizosphere of wheat. FEMS Microbiol. Ecol. 18:1995;129-138.
15. Kallmbach S., Manz W., Szewzyk U. Dynamics of biofilm formation in drinking water: phylogenetic affiliation and metabolic potential of single cells assessed by formazan reduction and in situ hybridization. FEMS Microbiol. Ecol. 22:1997;265-279.
16. Kaprelyants A.S., Kell D.B. Dormancy in stationary-phase cultures of Micrococcus luteus: flow cytometric analysis of starvation and resuscitation. Appl. Environ. Microbiol. 59:1993;3187-3196.
17. Karner M., Furhman J.A. Determination of active marine bacterioplankton: a comparison of universal 16S rRNA probes, autoradiography, and nucleoid staining. Appl. Environ. Microbiol. 63:1997;1208-1213.
18. Kogure K., Simidu U., Taga N. A tentative direct microscopic method for counting living marine bacteria. Can. J. Microbiol. 25:1979;415-420.
19. Lopez-Amoros R., Mason D.J., Lloyd D. Use of two oxonols and a fluorescent tetrazolium dye to monitor starvation of Escherichia coli in seawater by flow cytometry. J. Microbiol. Methods. 22:1995;165-176.
20. Lovejoy C., Legendre L., Therriault J.C., Tremblay J.E., Klein B., Ingram R.G. Growth and distribution of marine bacteria in relation to nanoplankton community structure. Deep-sea Res. 47:2000;461-487.
21. Mayr C., Winding A., Hendriksen N.B. Community-level physiological profile of soil bacteria unaffected by extraction method. J. Microbiol. Methods. 36:1999;29-33.
22. Proctor L.M., Souza A.C. Method for enumeration of 5-Cyano-2,3-Ditolyl tetrazolium Chloride (CTC)-active cells and cell specific CTC activity of benthic bacteria in riverine, estuarine and coastal sediment. J. Microbiol. Methods. 43:2001;213-222.
23. Pyle H.B., Broadaway S.C., McFeters G.A. Factors affecting the determination of respiratory activity on the basis of cyanoditolyl tetrazolium chloride reduction with membrane filtration. Appl. Environ. Microbiol. 61:1995;4304-4309.
24. Pyle B.H., Broadaway S.C., McFeters G.A. A rapid, direct method for enumerating respiring enterohemorrhagic Escherichia coli O157: H7 in water. Appl. Environ. Microbiol. 61:1995;2614-2619.
25. Rodriguez G.G., Phipps D., Ishiguro K., Ridgway H.F. Use of a fluorescent redox probe for direct visualization of actively respiring bacteria. Appl. Environ. Microbiol. 58:1992;1801-1808.
26. Schaule G., Flemming H.C., Ridgway H.F. Use of 5-cyano-2,3-ditolyl tetrazolium chloride for quantifying planktonic and sessile respiring bacteria in drinking water. Appl. Environ. Microbiol. 59:1993;3850-3857.
27. Servais P., Agogue H., Courties C., Joux F., Lebaron P. Are the actively respiring cells (CTC positive) those responsible for bacterial production in aquatic environments? FEMS Microbiol. Ecol. 35:2001;171-179.
28. Sherr B.F., del Giorgio P., Sherr E.B. Estimating abundance and single-cell characteristics of respiring bacteria via the redox dye CTC. Aquat. Microb. Ecol. 18:1999;117-131.
29. Sherr E.B., Sherr B.F., Sigmon C.T. Activity of marine bacteria under incubated and in situ conditions. Aquat. Microb. Ecol. 20:1999;213-223.
30. Sieracki M.E., Cucci T.L., Nicinski J. Flow cytometric analysis of 5-cyano-2,3-ditolyl tetrazolium chloride activity of marine bacterioplankton in dilution cultures. Appl. Environ. Microbiol. 65:1999;2409-2417.
31. Smith J.J., McFeters G.A. Mechanisms of INT (2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl tetrazolium chloride), and CTC (5-cyano-2,3-ditolyl-tetrazolium chloride) reduction in Escherichia coli K-12. J. Microbiol. Methods. 29:1997;161-175.
32. Smith E. Coherence of microbial respiration rate and cell-specific bacterial activity in a coastal planktonic community. Aquat. Microb. Ecol. 16:1998;27-35.
33. Suller M.T.E., Lloyd D. Fluorescence monitoring of antibiotic-induced bacterial damage using flow cytometry. Cytometry. 35:1999;235-241.
34. Ullrich S., Karrash B., Hoppe H.G., Jeskulke K., Mehrens M. Toxic effects on bacterial metabolism of the redox dye 5-cyano-2,3 dytolyl tetrazolium chloride. Appl. Environ. Microbiol. 62:1996;4587-4593.
35. Van Duyl F.C., De Winder B., Kop A.J., Wollenzien U. Tidal coupling between carbohydrate concentrations and bacterial activities in diatom-inhabited intertidal mud flats. Mar. Ecol., Progr. Ser. 191:1999;19-32.
36. Walsh S., Lappin-Scott H.M., Stockdale H., Herbert B.N. An assessment of the metabolic activity of starved and vegetative bacteria using two redox dyes. J. Microbiol. Methods. 24:1995;1-9.
37. Winding A., Binnerup S.J., Sorensen J. Viability of indigenous soil bacteria assayed by respiratory activity and growth. Appl. Environ. Microbiol. 60:1994;2869-2875.
38. Yamaguchi N., Nasu M. Flow cytometric analysis of bacterial respiratory and enzymatic activity in the natural aquatic environment. J. Appl. Microbiol. 83:1997;43-52.
39. Yu F.P., McFeters G.A. Rapid in situ assessment of physiological activities in bacterial biofilms using fluorescent probes. J. Microbiol. Methods. 20:1994;1-10.
40. Yu W., Dodds W.K., Banks M.K., Skalsky J., Strauss E.A. Optimal staining and sample storage time for direct microscopic enumeration of total and active bacteria in soil with two fluorescent dyes. Appl. Environ. Microbiol. 61:1995;3367-3372.