LIVE/DEAD(®) BacLight(TM): Application of a new rapid staining method for direct enumeration of viable and total bacteria in drinking water

Journal of Microbiological Methods

Volumn 37, Issue 1, 1999, Pages 77-86

  Boulos, Lina ^a   Prévost, Michèle ^a   Barbeau, Benoit ^a   Coallier, Josée ^a   Desjardins, Raymond ^a  

^a ÉCOLE POLYTECHNIQUE DE MONTRÉAL   (Canada)

Author keywords

BacLight; Culturability; Direct viable counts; Drinking water; Epifluorescence

Indexed keywords

DRINKING WATER; PROPIDIUM IODIDE; CHLORINE;

ARTICLE; BACTERIAL COLONIZATION; BACTERIAL COUNT; BACTERIUM DETECTION; CITROBACTER FREUNDII; ESCHERICHIA COLI; FLUORESCENCE; NONHUMAN; PRIORITY JOURNAL; STAINING; WATER ANALYSIS; WATER CONTAMINATION; ANALYTICAL EQUIPMENT; COMPARATIVE STUDY; DRUG EFFECT; INSTRUMENTATION; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; STANDARD; TIME; WATER SUPPLY;

CHLORINE; CITROBACTER FREUNDII; COLONY COUNT, MICROBIAL; REAGENT KITS, DIAGNOSTIC; STAINING AND LABELING; TIME FACTORS; WATER MICROBIOLOGY; WATER SUPPLY;

EID: 0032587252     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(99)00048-2     Document Type: Article

References (33)

1. AFNOR, 1987. Essais des eaux: dosage du chlore libre et du chlore total par la méthode colorimétrique à la DPD. French Normalization Association, NTF-90-038.
2. APHA, AWWA and WPCF, 1992. Standard Methods for the Examination of Water and Wastewater, 18th ed. APHA, Washington, DC, 1527 p.
3. Amy P.S., Pauling C., Morita R.Y. Recovery from nutrient starvation by a marine Vibrio sp. Appl. Environ. Microbiol. 45:1983;1685-1690.
4. Berg J.D., Roberts P.V., Matin A. Growth of disinfection-resistant bacteria and simulation of natural aquatic environments in the chemostat. Water Chlor. Chem. Environ. Impact Health Effects. 4:1985;1137-1147.
5. Braux A.S., Minet J., Tamanai-Shacoori Z., Riou G., Cormier M. Direct enumeration of injured Escherichia coli cells harvested onto membrane filters. J. Microbiol. Methods. 31:1997;1-8.
6. Coallier J., Prévost M., Rompré A. The optimization and application of two direct viable count methods for bacteria in distributed drinking water. Can. J. Microbiol. 40:1994;830-836.
7. Coallier J., Rompré A., Prévost M., Duchesne D. Viable counts: new methods to measure active bacteria in distribution systems. Proceedings of the WQTC-AWWA Proceedings (I), Miami, FL. 1993.
8. DuPreez M., Kfir R., Coubrough P. Investigation of injury of coliforms after chlorination. Water Sci. Technol. 31:1995;115-118.
9. Fogel D., Isaac-Renton J., Guasparini R., Moorehead W., Ongerth J. Removing Giardia and Cryptosporidium by slow sand filtration. J. Am. Water Works. 85:1993;77-84.
10. Hobbie J.E., Daley R.H., Jasper S. Use of Nuclepore filters for counting bacteria by fluorescence microscopy. Appl. Environ. Microbiol. 33:1977;1225-1228.
11. Joret J.C., Cervantes P., Levi Y., Dumoutier N., Cognet L., Hasley C., Husson M.O., Leclerc H. Rapid detection of E. coli in water using monoclonal antibodies. Water Sci. Technol. 21:1989;161-167.
12. Kaprelyants A.S., Kell D.B. Rapid assessment of bacterial viability and vitality by rhodamine 123 and flow cytometry. J. Appl. Bacteriol. 72:1992;410-422.
13. Kfir R., du Preez M., Genthe B. The use of monoclonal antibodies for the detection of fecal bacteria in water. Water Sci. Technol. 27:1993;257-260.
14. Kogure K., Simidu U., Taga N. A tentative direct microscopic method for counting living marine bacteria. Can. J. Microbiol. 25:1979;415-420.
15. LeChevallier M.W., Norton W.D. Examining relationships between particle counts and Giardia, Cryptosporidium and turbidity. J. Am. Water Works. 1992;54-60. December.
16. Lisle J.T., Broadway S.C., Prescott A.M., Pyle B.H., Fricker C., McFeters G.A. Effects of starvation on physiological activity and chlorine disinfection resistance in Escherichia coli O157:H7. Appl. Environ. Microbiol. 64:1998;4658-4662.
17. López-Amorós R., Comas J., Vives-Rego J. Flow cytometric assessment of Escherichia coli and Salmonella typhimurium starvation-survival in seawater using Rhodamine 123, propidium iodide, and oxonol. Appl. Environ. Microbiol. 61:1995;2521-2526.
18. McFeters G.A. Enumeration, occurrence and significance of injured indicator bacteria in drinking water. McFeters G.A. Drinking Water Microbiology: Progress and Recent Developments. 1990;Science Technology, Madison, WI.
19. Molecular Probes Live/Dead BacLight™ bacteria viability kit technical sheet. Molecular Probes Inc., 1995.
20. Morgan J.A.W., Rhodes G., Pickup R.W. Survival of nonculturable Aeromonas salmonicida in lake water. Appl. Environ. Microbiol. 59:1993;874-880.
21. Nieminski E.C. Giardia & Cryptosporidium - Where do the cysts go? Proceedings of the WQTC-AWWA Conference, Toronto, Ontario. 1992;205-217. Part I.
22. Olson B.H., Bradford F.M., Palmer C.J., Clark D.L., Murphy D. Rapid nutrient assessment techniques to determination of microbial stimulation from potable water. Proceedings of the Séminaire international de la MOB dans les réseaux de distribution d'eau potable, Nancy, France. 1991.
23. Porter K.G., Freig Y.S. The use of DAPI for identification and counting of aquatic microflora dimnil. Oceanography. 25:1980;943-948.
24. Pyle B.H., Broadway S.C., McFeters G.A. Factors affecting the determination of respiratory activity on the basis of cyanoditolyl tetrazolium chloride reduction with membrane filtration. Appl. Environ. Microbiol. 61:1995;4304-4309.
25. Rigsbee W., Simpson L.M., Oliver J.D. Detection of the viable but nonculturable state in Escherichia coli O157:H7. J. Food Safety. 16:1997;255-262.
26. Rodriguez G.G., Phipps D., Ishiguro K., Ridgway H.F. Use of a fluorescent redox probe for direct visualization of actively respiring bacteria. Appl. Environ. Microbiol. 58:1992;1801-1808.
27. Rose J.B., Darbin H., Gerba C.P. Correlations of the protozoa, Cryptosporidium and Giardia, with water quality variables in a watershed. Water Sci. Technol. 20:1988;271-276.
28. Roszak D.B., Colwell R.R. Survival strategies of bacteria in the natural environment. Microbiol. Rev. 51:1987;365-379.
29. Singh A., Yu F.-P., McFeters G.A. Rapid detection of chlorine induced bacterial injury by the direct viable count method using image analysis. Appl. Environ. Microbiol. 56:1990;389-394.
30. Vandemark P.J., Batzing B.L. The Microbes. An Introduction To Their Nature and Importance. 1987;182 Benjamin Cummings. Chapter 5.
31. Venkobachar C., Iyengar L., PrabhakaraRao A.V.S. Mechanism of disinfection: effect of chlorine on cell membrane functions. Water Res. 11:1977;727-729.
32. Yu F.P., McFeters G.A. Rapid in situ assessment of physiological activities in bacterial biofilms using fluorescent probes. J. Microbiol. Methods. 20:1994;1-10.
33. Zimmerman R., Iturriaga R., Becker-Birck J. Simultaneous determination of the total number of aquatic bacteria and the number thereof involved in respiration. Appl. Environ. Microbiol. 36:1978;926-935.