Bacterial DNA decontamination for reverse transcription polymerase chain reaction (RT-PCR)

Journal of Microbiological Methods

Volumn 51, Issue 1, 2002, Pages 119-121

  Wang, Gehua ^a   Barton, Connie ^a   Rodgers, Frank G ^a,b  

^a PUBLIC HEALTH AGENCY OF CANADA   (Canada)

^b UNIVERSITY OF NEW HAMPSHIRE   (United States)

Author keywords

Bacterial DNA decontamination; Manganese sulfate; Microvolume digestion; RNA extraction; RT PCR

Indexed keywords

BACTERIAL DNA; DEOXYRIBONUCLEASE; GENOMIC DNA; MAGNESIUM; MANGANESE SULFATE; NUCLEIC ACID; RIBONUCLEASE; RNA;

ANALYTIC METHOD; ARTICLE; CONTROLLED STUDY; DEVICE; INTERMETHOD COMPARISON; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION;

BUFFERS; DEOXYRIBONUCLEASE I; DNA, BACTERIAL; ESCHERICHIA COLI O157; MANGANESE COMPOUNDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, BACTERIAL; SULFATES;

BACTERIA (MICROORGANISMS);

EID: 0036280988     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(02)00041-6     Document Type: Article

References (9)

1. Use of manganese in RT-PCR eliminates PCR artifacts resulting from DNase I digestion
2. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
3. Single-step method of RNA isolation by acid-guanidinium thiocyanate-phenol-chloroform extraction
4. A rapid procedure for isolating RNA from small-scale bacillus cultures
5. A new procedure for efficient recovery of DNA, RNA, and proteins from listeria cells by rapid lysis with a recombinant bacteriophage endolysin
6. RT-PCR artifacts from processed pseudogenes
7. Pitfalls of processed pseudogenes in RT-PCR
8. Detection of mRNA by reverse transcription-PCR as an indicator of viability in Escherichia coli cells