PM2.5-induced oxidative stress triggers autophagy in human lung epithelial A549 cells

Toxicology in Vitro

Volumn 27, Issue 6, 2013, Pages 1762-1770

  Deng, Xiaobei ^a   Zhang, Fang ^a   Rui, Wei ^a   Long, Fang ^a   Wang, Lijuan ^b   Feng, Zhaohan ^c   Chen, Deliang ^a   Ding, Wenjun ^a  

^a UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^b INSTITUTE OF ZOOLOGY   (China)

^c CHINA JAPAN FRIENDSHIP HOSPITAL   (China)

Author keywords

Autophagy; Lung epithelial A549 cells; Oxidative stress; PM2.5

Indexed keywords

AUTOPHAGY PROTEIN 5; BECLIN 1; CATALASE; LACTATE DEHYDROGENASE; MESSENGER RNA; MICROTUBULE ASSOCIATED PROTEIN LIGHT CHAIN 3; PROTEIN; REACTIVE OXYGEN METABOLITE; SUPEROXIDE DISMUTASE; UNCLASSIFIED DRUG; 4',6 DIAMIDINO 2 PHENYLINDOLE; ELEMENTAL CARBON; HYDROXYL RADICAL; LC3; MEFECLORAZINE; ORGANIC CARBON; SUPEROXIDE; AIR POLLUTANT; PARTICULATE MATTER;

A549 CELL; AIR POLLUTION; ARTICLE; AUTOPHAGY; CARDIOVASCULAR DISEASE; CAUSE OF DEATH; CELL LINE; CELL VIABILITY; CONTROLLED STUDY; ENVIRONMENTAL EXPOSURE; ENZYME ACTIVITY; ENZYME RELEASE; HOSPITAL ADMISSION; HUMAN; HUMAN CELL; LUNG ALVEOLUS EPITHELIUM; LUNG CANCER; LUNG FUNCTION; OXIDATIVE STRESS; PARTICLE SIZE; PARTICULATE MATTER; RESPIRATORY TRACT DISEASE; TOXICITY; 2′,7′-DICHLORODIHYDROFLUORESCEIN DIACETATE; 2′,7′-DICHLOROFLUORESCEIN; 4,6-DIAMIDO-2-PHENYLINDOLE HYDROCHLORIDE; AIR POLLUTANT; AMBIENT AIRBORNE PARTICULATE MATTER; CYTOLOGY; DCFH-DA; DRUG EFFECT; EC; EPITHELIUM CELL; LUNG; LUNG EPITHELIAL A549 CELLS; METABOLISM; MICROTUBULE-ASSOCIATED PROTEINS LIGHT CHAIN 3; N-ACETYLCYSTEINE; NAC; OC; OH(); PAHS; PM; PM(2.5); POLYCYCLIC AROMATIC HYDROCARBONS; ROS; SCANNING ELECTRON MICROSCOPY; TEM; TRANSMISSION ELECTRON MICROSCOPY; TUMOR CELL LINE; DRUG EFFECTS;

2′,7′-DICHLORODIHYDROFLUORESCEIN DIACETATE; 2′,7′-DICHLOROFLUORESCEIN; 4,6-DIAMIDO-2-PHENYLINDOLE HYDROCHLORIDE; AMBIENT AIRBORNE PARTICULATE MATTER; AUTOPHAGY; DAPI; DCF; DCFH-DA; EC; ELEMENTAL CARBON; HYDROXYL RADICAL; LACTATE DEHYDROGENASE; LC3; LDH; LUNG EPITHELIAL A549 CELLS; MICROTUBULE-ASSOCIATED PROTEINS LIGHT CHAIN 3; N-ACETYLCYSTEINE; NAC; OC; OH(); ORGANIC CARBON; OXIDATIVE STRESS; PAHS; PM; PM(2.5); POLYCYCLIC AROMATIC HYDROCARBONS; REACTIVE OXYGEN SPECIES; ROS; SCANNING ELECTRON MICROSCOPY; SEM; SUPEROXIDE ANION; TEM; TRANSMISSION ELECTRON MICROSCOPY;

AIR POLLUTANTS; AUTOPHAGY; CATALASE; CELL LINE, TUMOR; EPITHELIAL CELLS; HUMANS; L-LACTATE DEHYDROGENASE; LUNG; OXIDATIVE STRESS; PARTICULATE MATTER; REACTIVE OXYGEN SPECIES; SUPEROXIDE DISMUTASE;

EID: 84879352101     PISSN: 08872333     EISSN: 18793177     Source Type: Journal    
DOI: 10.1016/j.tiv.2013.05.004     Document Type: Article

References (41)

1. Abounit K., Scarabelli T.M., McCauley R.B. Autophagy in mammalian cells. World Journal of Biological Chemistry 2012, 3:1-6.
2. Anderson J.O., Thundiyil J.G., Stolbach A. Clearing the air: a review of the effects of particulate matter air pollution on human health. Journal of Medical Toxicology 2012, 8:166-175.
3. Azad M.B., Chen Y., Gibson S.B. Regulation of autophagy by reactive oxygen species (ROS): implications for cancer progression and treatment. Antioxidants & Redox Signaling 2009, 11:777-790.
4. 2.5): physicochemical characterization and metabolic activation of the organic fraction in human lung epithelial cells (A549). Environmental Research 2007, 105:212-223.
5. Brigelius-Flohé R. Commentary: oxidative stress reconsidered. Genes & Nutrition 2009, 4:161-163.
6. Brunekreef B., Holgate S.T. Air pollution and health. Lancet 2002, 360:1233-1242.
7. Cao J., Chow J.C., Lee S., Li Y., Chen S., An Z., Fung K., Watson J., Zhu C., Liu S. Characterization and source apportionment of atmospheric organic and elemental carbon during fall and winter of 2003 in Xi'an, China. Atmospheric Chemistry and Physics Discussions 2005, 5:3561-3593.
8. Chen Y., McMillan-Ward E., Kong J., Israels S., Gibson S. Oxidative stress induces autophagic cell death independent of apoptosis in transformed and cancer cells. Cell Death & Differentiation 2007, 15:171-182.
9. Chen L.H., Chu P.M., Lee Y.J., Tu P.H., Chi C.W., Lee H.C., Chiou S.H. Targeting protective autophagy exacerbates UV-triggered apoptotic cell death. International Journal of Molecular Sciences 2012, 13:1209-1224.
10. 10 impairs the antioxidant defense system and exacerbates oxidative stress driven cell death. Toxicology Letters 2010, 193:209-216.
11. 10 at regionally representative locations during SJVAQS/AUSPEX. Atmospheric Environment 1996, 30:2079-2112.
12. Fridovich I. Biological effects of the superoxide radical. Archives of Biochemistry and Biophysics 1986, 247:1-11.
13. 2.5. Toxicology in Vitro 2006, 20:575-584.
14. Ghio A.J., Carraway M.S., Madden M.C. Composition of air pollution particles and oxidative stress in cells, tissues, and living systems. Journal of Toxicology and Environmental Health, Part B 2012, 15:1-21.
15. Gualtieri M., Mantecca P., Corvaja V., Longhin E., Perrone M.G., Bolzacchini E., Camatini M. Winter fine particulate matter from Milan induces morphological and functional alterations in human pulmonary epithelial cells (A549). Toxicology Letters 2009, 188:52-62.
16. Gualtieri M., Øvrevik J., Holme J.A., Perrone M.G., Bolzacchini E., Schwarze P.E., Camatini M. Differences in cytotoxicity versus pro-inflammatory potency of different PM fractions in human epithelial lung cells. Toxicology in Vitro 2010, 24:29-39.
17. 2.5. Toxicology Letters 2012, 209:136-145.
18. Harrison R.M., Yin J. Particulate matter in the atmosphere: which particle properties are important for its effects on health?. Science of the Total Environment 2000, 249:85-101.
19. Imrich A., Ning Y.Y., Kobzik L. Insoluble components of concentrated air particles mediate alveolar macrophage responses in vitro. Toxicology and Applied Pharmacology 2000, 167:140-150.
20. Kiffin R., Bandyopadhyay U., Cuervo A.M. Oxidative stress and autophagy. Antioxidants & Redox Signaling 2006, 8:152-162.
21. Kiyono K., Suzuki H.I., Matsuyama H., Morishita Y., Komuro A., Kano M.R., Sugimoto K., Miyazono K. Autophagy is activated by TGF-β and potentiates TGF-β-mediated growth inhibition in human hepatocellular carcinoma cells. Cancer Research 2009, 69:8844-8852.
22. Knaapen A.M., Borm P.J.A., Albrecht C., Schins R.P.F. Inhaled particles and lung cancer. Part A: Mechanisms. International Journal of Cancer 2004, 109:799-809.
23. Koh M., Kim H.J. The effects of metallothionein on the activity of enzymes involved in removal of reactive oxygen species. Bulletin-Korean Chemical Society 2001, 22:362-366.
24. Kondo Y., Kondo S. Autophagy and cancer therapy. Autophagy 2006, 2:85-90.
25. 2.5) collected in Abidjan, CÔte d'Ivoire. Journal of Applied Toxicology 2010, 30:310-320.
26. Lee Y.S., Kim D.W., Lee Y.H., Oh J.H., Yoon S., Choi M.S., Lee S.K., Kim J.W., Lee K., Song C.W. Silver nanoparticles induce apoptosis and G2/M arrest via PKCζ-dependent signaling in A549 lung cells. Archives of Toxicology 2011, 85:1529-1540.
27. Leeuwenburgh C., Heinecke J. Oxidative stress and antioxidants in exercise. Current Medicinal Chemistry 2001, 8:829-838.
28. Levy J.I., Hammitt J.K., Spengler J.D. Estimating the mortality impacts of particulate matter: what can be learned from between-study variability?. Environmental Health Perspectives 2000, 108:109-117.
29. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods 1983, 65:55-63.
30. Pamplona R., Costantini D. Molecular and structural antioxidant defenses against oxidative stress in animals. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 2011, 301:R843-863.
31. Pope C.A., Burnett R.T., Thun M.J., Calle E.E., Krewski D., Ito K., Thurston G.D. Lung cancer, cardiopulmonary mortality, and long-term exposure to fine particulate air pollution. JAMA: the Journal of the American Medical Association 2002, 287:1132-1141.
32. 2.5) can induce acute oxidative stress, inflammation and pulmonary impairment in healthy mice. Inhalation Toxicology 2011, 23:257-267.
33. Van Limbergen J., Stevens C., Nimmo E., Wilson D., Satsangi J. Autophagy: from basic science to clinical application. Mucosal Immunology 2009, 2:315-330.
34. Viola G., Bortolozzi R., Hamel E., Moro S., Brun P., Castagliuolo I., Ferlin M.G., Basso G. MG-2477, a new tubulin inhibitor, induces autophagy through inhibition of the Akt/mTOR pathway and delayed apoptosis in A549 cells. Biochemical Pharmacology 2011, 83:16-26.
35. Wei H., Wei D., Yi S., Zhang F., Ding W. Oxidative stress induced by urban fine particles in cultured EA. hy926 cells. Human & Experimental Toxicology 2011, 30:579-590.
36. White E., DiPaola R.S. The double-edged sword of autophagy modulation in cancer. Clinical Cancer Research 2009, 15:5308-5316.
37. Wu W.-S. The signaling mechanism of ROS in tumor progression. Cancer and Metastasis Reviews 2006, 25:695-705.
38. 10) induces oxidative stress in human lung epithelial A549 cells. Environmental Toxicology 2012, 10.1002/tox.21750.
39. Zhang Z.Z., Che W.J., Liang Y., Wu M., Li N., Shu Y., Liu F., Wu D.S. Comparison of cytotoxicity and genotoxicity induced by the extracts of methanol and gasoline engine exhausts. Toxicology in Vitro 2007, 21:1058-1065.
40. Zhang Y., Schauer J.J., Zeng L., Wei Y., Liu Y., Shao M. Characteristics of particulate carbon emissions from real-world Chinese coal combustion. Environmental Science & Technology 2008, 42:5068-5073.
41. Zhang Y.X., Schauer J.J., Stone E.A., Zhang Y., Shao M., Wei Y., Zhu X. Harmonizing molecular marker analyses of organic aerosols. Aerosol Science and Technology 2009, 43:275-283.