Gene expression profiling of A549 cells exposed to Milan PM2.5

Toxicology Letters

Volumn 209, Issue 2, 2012, Pages 136-145

  Gualtieri, Maurizio ^a   Longhin, Eleonora ^a   Mattioli, Michela ^b   Mantecca, Paride ^a   Tinaglia, Valentina ^b   Mangano, Eleonora ^c   Proverbio, Maria Carla ^b   Bestetti, Giuseppina ^a   Camatini, Marina ^a   Battaglia, Cristina ^b  

^a UNIVERSITY OF MILANO BICOCCA   (Italy)

^b UNIVERSITY OF MILAN   (Italy)

^c CNR   (Italy)

Author keywords

A549; DNA damage; Gene expression; PM2.5; ROS

Indexed keywords

ANTIGEN; CYTOCHROME P450 1A1; CYTOCHROME P450 1B1; DNA; EPIREGULIN; FOS LIKE ANTIGEN1; REACTIVE OXYGEN METABOLITE; UNCLASSIFIED DRUG;

ARTICLE; CELL VIABILITY; CONTROLLED STUDY; CYTOTOXICITY; DNA DAMAGE; DNA STRAND BREAKAGE; GENE EXPRESSION; GENE EXPRESSION PROFILING; HUMAN; HUMAN CELL; IN VITRO STUDY; INTRACELLULAR SIGNALING; ITALY; MICROARRAY ANALYSIS; PARTICULATE MATTER; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SEASONAL VARIATION; SIGNAL TRANSDUCTION; SUMMER; UPREGULATION; WINTER;

ARYL HYDROCARBON HYDROXYLASES; BLOTTING, WESTERN; CELL LINE; CELL SURVIVAL; COMET ASSAY; DNA DAMAGE; ENVIRONMENTAL EXPOSURE; EPITHELIAL CELLS; FLOW CYTOMETRY; GENE EXPRESSION; GENE EXPRESSION PROFILING; HUMANS; ITALY; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PARTICULATE MATTER; POLYCYCLIC HYDROCARBONS, AROMATIC; REACTIVE OXYGEN SPECIES; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; SEASONS; URBAN POPULATION;

EID: 84855513089     PISSN: 03784274     EISSN: 18793169     Source Type: Journal    
DOI: 10.1016/j.toxlet.2011.11.015     Document Type: Article

References (60)

1. Andrysik Z., Vondráček J., Marvanová S., Ciganek M., Neča J., Pěnčíková K., Mahadevan B., Topinka J., Baird W.M., Kozubík A., Machala M. Activation of the aryl hydrocarbon receptor is the major toxic mode of action of an organic extract of a reference urban dust particulate matter mixture: the role of polycyclic aromatic hydrocarbons. Mutat. Res. 2011, 714:53-62.
2. Arrieta D.E., Ontiveros C.C., Li W.W., Garcia J.H., Denison M.S., Mcdonald J.D., Burchiel S.W., Washburn B.S. Aryl hydrocarbon receptor-mediated activity of particulate organic matter from the Paso del Norte Airshed along the U.S.-Mexico border. Environ. Health Perspect. 2003, 111(10):1299-1305.
3. Becker S., Dailey L.A., Soukup J.M., Grambow S.C., Devlin R.B., Huang Y.C.T. Seasonal variations in air pollution particle-induced inflammatory mediator release and oxidative stress. Environ. Health Perspect. 2005, 113(8):1032-1038.
4. Billet S., Garçon G., Dagher Z., Verdin A., Ledoux F., Cazier F., Courcot D., Aboukais A., Shirali P. Ambient particulate matter (PM2.5): physicochemical characterization and metabolic activation of the organic fraction in human lung epithelial cells (A549). Environ. Res. 2007, 105:212-223.
5. Billet S., Abbas I., Le Goff J., Verdin A., Andrè V., Lafargue P.E., Hachimi A., Cazier F., Sichel F., Shirali P., Garcon P. Genotoxic potential of polycyclic aromatic hydrocarbons-coated onto airborne particulate matter (PM2.5) in human lung epithelial A549 cells. Cancer Lett. 2008, 270:144-155.
6. Binkova B., Vesely D., Vesela D., Jelinek R., Sram R.J. Genotoxicity and embryotoxicity of urban air particulate matter collected during winter and summer period in two different districts of the Czech Republic. Mutat. Res. 1999, 440:45-58.
7. Bonetta S., Gianotti V., Bonetta S., Gosetti F., Oddone M., Gennaro M.C., Carraio E. DNA damage in A549 cells exposed to different extracts of PM2.5 from industrial, urban and highway sites. Chemosphere 2009, 77:1030-1034.
8. Camatini M., Corvaja V., Pezzolato E., Mantecca P., Gualtieri M. PM10-biogenic fraction drives the seasonal variation of proinflammatory response in A549 cells. Environ. Toxicol. 2010, 10.1002/tox.20611.
9. Castano-Vinyals G., D'Errico A., Malats N., Kogevinas M. Biomarkers of exposure to polycyclic aromatic hydrocarbons from environmental air pollution. Occup. Environ. Med. 2004, 61:12.
10. Castorena-Torres F., Bermúdez de León M., Cisneros B., Zapata-Pérez O., Salinas J.E., Albores A. Changes in gene expression induced by polycyclic aromatic hydrocarbons in the human cell lines HepG2 and A549. Toxicol. In Vitro 2008, 22(2):411-421.
11. Costello C.M., Howell K., Cahill E., McBryan J., Konigshoff M., Eickelberg O., Gaine S., Martin F., McLoughlin P. Lung-selective gene responses to alveolar hypoxia: potential role for the bone morphogenetic antagonist gremlin in pulmonary hypertension. Am. J. Physiol. Lung Cell. Mol. Physiol. 2008, 295:L272-L284.
12. Dagher Z., Garçon G., Billet S., Gosset P., Ledoux F., Courcot D., Aboukais A., Puskaric E., Shirali P. Activation of different pathways of apoptosis by Dunkerque city air pollution particulate matter (PM2.5) in human epithelial lung cells (L132) in culture. Toxicology 2006, 225:12-24.
13. Diani-Moore S., Ram P., Li X., Mondal P., Youn D.Y., Sauve A.A., Rifkind A.B. Identification of the aryl hydrocarbon receptor target GeneTiPARP as a mediator of suppression of hepatic gluconeogenesis by 2,3,7,8-tetrachlorodibenzo-p-dioxin and of nicotinamide as a corrective agent for this effect. J. Biol. Chem. 2010, 285:38801-38810.
14. Englert N. Fine particles and human health-a review of epidemiological studies. Toxicol. Lett. 2004, 149:235-242.
15. Farina F., Sancini G., Mantecca P., Gallinotti D., Camatini M., Palestini P. The acute toxic effects of particulate matter in mouse lung are related to size and season of collection. Toxicol. Lett. 2011, 202(3):209-217.
16. Ferecatu I., Borot M.C., Bossard C., Leroux M., Boggetto N., Marano F., Baeza-Squiban A., Andreau K. Polycyclic aromatic hydrocarbon components contribute to the mitochondria-antiapoptotic effect of fine particulate matter on human bronchial epithelial cells via the aryl hydrocarbon receptor. Part. Fibre Toxicol. 2010, 7:1.
17. Gualtieri M., Mantecca P., Corvaja V., Longhin L., Perrone M.G., Bolzacchini E., Camatini M. Winter fine particulate matter from Milan induces morphological and functional alterations in human pulmonary epithelial cells (A549). Toxicol. Lett. 2009, 188(1):52-62.
18. Gualtieri M., Øvrevik J., Holme J.A., Perrone M.G., Bolzacchini E., Schwarze P.E., Camatini M. Differences in cytotoxicity versus pro-inflammatory potency of different PM fractions in human epithelial lung cells. Toxicol. In Vitro 2010, 24:29-39.
19. Gualtieri M., Øvrevik J., Mollerup S., Asare N., Longhin E., Dahlman H.J., Camatini M., Holme J.A. Airborne urban particles (Milan winter-PM2.5) cause mitotic arrest and cell death: effects on DNA, mitochondria, AhR binding and spindle organization. Mutat. Res. 2011, 713:18-31.
20. Gutierrez-Castillo M.E., Roubicek D.A., Cebrian-Garcia M.E., Vizcaya-Ruiz A.D., Sordo-Cedeno M., Ostrosky-Wegman P. Effect of chemical composition on the induction of DNA damage by urban airborne particulate matter. Environ. Mol. Mutagen. 2006, 47:199-211.
21. Gwinn M.R., Weston A. Application of oligonucleotide microarray technology to toxic occupational exposures. J. Toxicol. Environ. Health A 2008, 71(5):315-324.
22. Hetland R.B., Cassee F.R., Refsnes M., Schwarze P.E., Låg M., Boere A.J.F., Dybing E. Release of inflammatory cytokines, cell toxicity and apoptosis in epithelial lung cells after exposure to ambient air particles of different size fractions. Toxicol. In Vitro 2004, 18:203-212.
23. Hockley S.L., Arlt V.M., Brewer D., Giddings I., Phillips D.H. Time- and concentration-dependent changes in gene expression induced by benzo[a]pyrene in two human cell lines, MCF-7 and HepG2. BMC Genomics 2006, 7:260.
24. Hockley S.L., Arlt V.M., Brewer D., Te Poele R., Workman P., Giddings I., Phillips D.H. AHR- and DNA-damage-mediated gene expression responses induced by benzo(a)pyrene in human cell lines. Chem. Res. Toxicol. 2007, 20:1797-1810.
25. Huang D.W., Sherman B.T., Lempicki R.A. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat. Protoc. 2009, 4(1):44-57.
26. Irizarry R.A., Hobbs B., Collin F., Beazer-Barclay Y.D., Antonellis K.J., Scherf U., Speed T.P. Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics 2003, 4(2):249-264.
27. Karlsson H.L., Ljungman A.G., Lindbom J., Moller L. Comparison of genotoxic and inflammatory effects of particles generated by wood combustion, a road simulator and collected from street and subway. Toxicol. Lett. 2006, 165:203-211.
28. Koelemeijer R.B.A., Homan C.D., Matthijsen J. Comparison of spatial and temporal variations of aerosol optical thickness and particulate matter over Europe. Atmos. Environ. 2006, 40:5304-5315.
29. Kometani T., Yoshino I., Miura N., Okazaki H., Ohba T., Takenaka T., Shoji F., Yano T., Maehara Y. Benzo[a]pyrene promotes proliferation of human lung cancer cells by accelerating the epidermal growth factor receptor signaling pathway. Cancer Lett. 2009, 278(1):27-33.
30. Krewski D., Burnett R., Jerrett M., Pope C.A., Rainham D., Calle E., Thurston G., Thun M. Mortality and long-term exposure to ambient air pollution: ongoing analyses based on the American Cancer Society cohort. J. Toxicol. Environ. Health Part A 2005, 68:1093-1109.
31. Liu H., Dong H., Robertson K., Liu C. DNA methylation suppresses expression of the urea cycle enzyme carbamoyl phosphate synthetase 1 (CPS1) in human hepatocellular carcinoma. Am. J. Pathol. 2011, 178:652-661.
32. Lonati G., Ozgen G., Giugliano M. Primary and secondary carbonaceous species in PM2.5 samples in Milan (Italy). Atmos. Environ. 2007, 41:4599-4610.
33. Lonati G., Giugliano M., Ozgen S. Primary and secondary components of PM2.5 in Milan (Italy). Environ. Int. 2008, 34:665-670.
34. Ma Q. Induction and superinduction of 2,3,7,8-tetrachlorodibenzo-p-dioxin-inducible poly(ADP-ribose) polymerase: role of the aryl hydrocarbon receptor/aryl hydrocarbon receptor nuclear translocator transcription activation domains and a labile transcription repressor. Arch. Biochem. Biophys. 2002, 404(2):309-316.
35. Mahadevan B., Parsons H., Musafia T., Sharma A.K., Amin S., Pereira C., Baird W.M. Effect of artificial mixtures of environmental polycyclic aromatic hydrocarbons present in coal tar, urban dust, and diesel exhaust particulates on MCF-7 cells in culture. Environ. Mol. Mutagen. 2004, 44:99-107.
36. Marcazzan G., Vaccaro S., Valli G., Vecchi R. Characterisation of PM10 and PM2.5 particulate matter in the ambient air of Milan (Italy). Atmos. Environ. 2001, 35(27):4639-4650.
37. Mehta M., Chen L.C., Gordon T., Rom W., Tang M.S. Particulate matter inhibits DNA repair and enhances mutagenesis. Mutat. Res. 2008, 657:116-121.
38. Myllärniemi M., Lindholm P., Ryynänen M.J., Kliment C.R., Salmenkivi K., Keski-Oja J., Kinnula V.L., Oury T.D., Koli K. Gremlin-mediated decrease in bone morphogenetic protein signaling promotes pulmonary fibrosis. Am. J. Respir. Crit. Care Med. 2008, 177(3):321-329.
39. Nakayama Wong L.S., Aung H.H., Lamé M.W., Wegesser T.C., Wilson D.W. Fine particulate matter from urban ambient and wildfire sources from California's San Joaquin Valley initiate differential inflammatory, oxidative stress, and xenobiotic responses in human bronchial epithelial cells. Toxicol. In Vitro 2011, 10.1016/j.tiv.2011.06.001.
40. Nerriere E., Zmirou-Navier D., Desqueyroux P., Leclerc N., Momas I., Czernichow P. Lung cancer risk assessment in relation with personal exposure to airborne particles in four French metropolitan areas. J. Occup. Environ. Med. 2005, 47:1211-1217.
41. Olivares A., van Drooge B.D., Ballesta P.P., Grimalt J.O., Piña B. Assessment of dioxin-like activity in ambient air particulate matter using recombinant yeast assays. Atmos. Environ. 2011, 45(1):271-274.
42. Pavanello S., Bollati V., Pesatori A.C., Kapka L., Bolognesi C., Bertazzi P.A., Baccarelli A. Global and gene-specific promoter methylation changes are related to anti-B[a]PDE-DNA adduct levels and influence micronuclei levels in polycyclic aromatic hydrocarbon-exposed individuals. Int. J. Cancer 2009, 125:1692-1697.
43. Perrone M.G., Gualtieri M., Ferrero L., Lo Porto C., Udisti R., Bolzacchini E., Camatini M. Seasonal variations in chemical composition and in vitro biological effects of fine PM from Milan. Chemosphere 2010, 78:1368-1377.
44. Pope C.A., Ezzati M., Dockery D.W. Fine-particulate air pollution and life expectancy in the United States. N. Engl. J. Med. 2009, 360:376-386.
45. Querol X., Alastuey A., Rodríguez S., Viana M.M., Artíñano B., Salvador P., Mantilla E., García do Santos S., Fernandez Patier R., de La Rosa J., Sanchez de la Campa A., Menéndez M., Gil J.J. Levels of particulate matter in rural, urban and industrial sites in Spain. Sci. Total Environ. 2004, 334:359-376.
46. Risom L., Møller P., Loft S. Oxidative stress-induced DNA damage by particulate air pollution. Mutat. Res. 2005, 592:119-137.
47. Samet J.M., De Marini D.M., Malling H.V. Do airborne particles induce heritable mutations?. Science 2004, 304:971-972.
48. Sanchez-Perez Y., Chirino Y.I., Osornio-Vargas A.R., Morales-Barcenas R., Gutierrez-Ruiz C., Vazquez-Lopez I., Garcia-Cuellar C.M. DNA damage response of A549 cells treated with particulate matter (PM10) of urban air pollutants. Cancer Lett. 2009, 278:192-200.
49. Schins R.P.F., Knaapen A.M., Weishaupt C., Winzer A., Borm P.J.A. Cytotoxic and inflammatory effects of coarse and fine particulate matter in macrophages and epithelial cells. Ann. Occup. Hyg. 2002, 46(1):203-206.
50. Sen B., Mahadevan B., De Marini D.M. Transcriptional responses to complex mixtures-a review. Mutat. Res. 2007, 636:144-177.
51. Sevastyanova O., Novakova Z., Hanzalova K., Binkova B., Sram R.J., Topinka J. Temporal variation in the genotoxic potential of urban air particulate matter. Mutat. Res. 2008, 649:179-186.
52. Sharma A.K., Jensen K.A., Rank J., White P.A., Lundstedt S., Gagnec R., Jacobsen N.R., Kristiansen J., Vogel U., Wallin H. Genotoxicity, inflammation and physico-chemical properties of fine particle samples from an incineration energy plant and urban air. Mutat. Res. 2007, 633:95-111.
53. Solhaug A., Refsnes M., Låg M., Schwarze P.E., Husùy T., Holme J.A. Polycyclic aromatic hydrocarbons induce both apoptotic and anti-apoptotic signals in Hepa1c1c7 cells. Carcinogenesis 2004, 25(5):809-819.
54. Somers C.M., McCarry B.E., James F.M., Quinn S. Reduction of particulate air pollution lowers the risk of heritable mutations in mice. Science 2004, 304:1008-1010.
55. Tian L., Suzuki M., Nakajima T., Kubo R., Sekine Y., Shibuya K., Hiroshima K., Nakatani Y., Fujisawa T., Yoshino I. Clinical significance of aberrant methylation of prostaglandin E receptor 2 (PTGER2) in nonsmall cell lung cancer. Cancer 2008, 113:1396-1403.
56. Topinka J., Schwarz L.R., Wiebel F.J., Cerna M., Wolff T. Genotoxicity of urban air pollutants in the Czech Republic. Part II. DNA adduct formation in mammalian cells by extractable organic matter. Mutat. Res. 2000, 469:83-93.
57. Wenger D., Gerecke A.C., Heeb N.V., Hueglin C., Seiler C., Haag R., Naegeli H., Zenobi R. Aryl hydrocarbon receptor-mediated activity of atmospheric particulate matter from an urban and a rural site in Switzerland. Atmos. Environ. 2009, 43(22-23):3556-3562.
58. Yoshino I., Kometani T., Shoji F., Osoegawa A., Ohba T., Kouso H., Takenaka T., Yohena T., Maehara Y. Induction of epithelial-mesenchymal transition-related genes by benzo[a]pyrene in lung cancer cells. Cancer 2007, 110:369-374.
59. Zanobetti A., Schwartz J. The effect of fine and coarse particulate air pollution on mortality: a national analysis. Environ. Health Perspect. 2009, 117(6):898-903.
60. Zerbi G., Ferruggiari A., Fustella G., Tommasini M., Mantecca P., Gualtieri M., Cetta F., Camatini M. Preliminary observation on the interactions between fine atmospheric particulate matter (PM2.5) and human alveolar epithelial cells (A549). Chem. Eng. Trans. 2008, 16:387-394.