How to perform RT-qPCR accurately in plant species? A case study on flower colour gene expression in an azalea (Rhododendron simsii hybrids) mapping population

BMC Molecular Biology

Volumn 14, Issue , 2013, Pages

  De Keyser, Ellen ^a   Desmet, Laurence ^a   Van Bockstaele, Erik ^a,b   De Riek, Jan ^a  

^a INSTITUTE FOR AGRICULTURAL AND FISHERIES RESEARCH ILVO   (Belgium)

^b GHENT UNIVERSITY   (Belgium)

Author keywords

Flower colour; Gene expression; noRT; Pink; Reference genes; RNA quality; RT qPCR; Standard curves

Indexed keywords

ANTHOCYANIDIN SYNTHASE; CHALCONE SYNTHASE; DIHYDROFLAVONOL 4 REDUCTASE; DNA; FLAVANONE 3 HYDROXYLASE; FLAVONOID; FLAVONOL; FLAVONOL SYNTHASE; RNA; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; DNA SEQUENCE; FLOWER; FLOWER COLOR; GENE AMPLIFICATION; GENE EXPRESSION; HYBRID; NONHUMAN; NUCLEOTIDE SEQUENCE; PETAL; PIGMENTATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RHODODENDRON SIMSII;

BIOSYNTHETIC PATHWAYS; COLOR; FLAVONOIDS; FLOWERS; GENE EXPRESSION REGULATION, PLANT; PIGMENTS, BIOLOGICAL; PLANT PROTEINS; REAL-TIME POLYMERASE CHAIN REACTION; RHODODENDRON;

RHODODENDRON SIMSII;

EID: 84879237553     PISSN: None     EISSN: 14712199     Source Type: Journal    
DOI: 10.1186/1471-2199-14-13     Document Type: Article

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