메뉴 건너뛰기




Volumn 27, Issue 3, 2009, Pages 407-416

Evaluation of candidate reference genes for real-time quantitative PCR of plant samples using purified cDNA as template

Author keywords

Arabidopsis thaliana; Gene expression; Microcapillary electrophoresis; Picea abies; Quantitative real time PCR analysis

Indexed keywords

ARABIDOPSIS; ARABIDOPSIS THALIANA; MAMMALIA; PICEA ABIES;

EID: 70350012072     PISSN: 07359640     EISSN: 15729818     Source Type: Journal    
DOI: 10.1007/s11105-008-0072-1     Document Type: Article
Times cited : (36)

References (47)
  • 1
    • 3442876969 scopus 로고    scopus 로고
    • Normalization of real-time quantitative reverse transcription-PCR data: A model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets
    • doi:10.1158/0008-5472.CAN-04-0496
    • Andersen CL, Jensen JL, Orntoft TF (2004) Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res 64:5245-5250 doi:10.1158/0008-5472.CAN-04-0496.
    • (2004) Cancer Res , vol.64 , pp. 5245-5250
    • Andersen, C.L.1    Jensen, J.L.2    Orntoft, T.F.3
  • 2
    • 0345257783 scopus 로고    scopus 로고
    • Chipping away at the chip bias: RNA degradation in microarray analysis
    • doi:10.1038/ng1203-292
    • Auer H, Lyianarachchi S, Newsom D, Klisovic MI, Marcucci G, Kornacker K (2003) Chipping away at the chip bias: RNA degradation in microarray analysis. Nat Genet 35:292-293 doi:10.1038/ng1203-292.
    • (2003) Nat Genet , vol.35 , pp. 292-293
    • Auer, H.1    Lyianarachchi, S.2    Newsom, D.3    Klisovic, M.I.4    Marcucci, G.5    Kornacker, K.6
  • 3
    • 1542266463 scopus 로고    scopus 로고
    • Kinetic outlier detection (KOD) in real-time PCR
    • doi:10.1093/nar/gng106
    • Bar T, Stahlberg A, Muszta A, Kubista M (2003) Kinetic outlier detection (KOD) in real-time PCR. Nucleic Acids Res 31:e105 doi:10.1093/nar/gng106.
    • (2003) Nucleic Acids Res , vol.31
    • Bar, T.1    Stahlberg, A.2    Muszta, A.3    Kubista, M.4
  • 4
    • 0035048415 scopus 로고    scopus 로고
    • Conifer genetic engineering: Transgenic Pinus radiata (D. Don) and Picea abies (Karst) plants are resistant to the herbicide Buster
    • doi:10.1007/s002990100317
    • Bishop-Hurley SL, Zabkiewicz RJ, Grace L, Gardner RC, Wagner A, Walter C (2001) Conifer genetic engineering: transgenic Pinus radiata (D. Don) and Picea abies (Karst) plants are resistant to the herbicide Buster. Plant Cell Rep 20:235-243 doi:10.1007/s002990100317.
    • (2001) Plant Cell Rep , vol.20 , pp. 235-243
    • Bishop-Hurley, S.L.1    Zabkiewicz, R.J.2    Grace, L.3    Gardner, R.C.4    Wagner, A.5    Walter, C.6
  • 5
    • 5444258598 scopus 로고    scopus 로고
    • Validating internal controls for quantitative plant gene expression studies
    • doi:10.1186/1471-2229-4-14
    • Brunner AM, Yakovlev IA, Strauss SH (2004) Validating internal controls for quantitative plant gene expression studies. BMC Plant Biol 4:14 doi:10.1186/1471-2229-4-14.
    • (2004) BMC Plant Biol , vol.4 , pp. 14
    • Brunner, A.M.1    Yakovlev, I.A.2    Strauss, S.H.3
  • 6
    • 0033775615 scopus 로고    scopus 로고
    • Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
    • doi:10.1677/jme.0.0250169
    • Bustin SA (2000) Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 25:169-193 doi:10.1677/jme.0.0250169.
    • (2000) J Mol Endocrinol , vol.25 , pp. 169-193
    • Bustin, S.A.1
  • 7
    • 1942475192 scopus 로고    scopus 로고
    • Real-time PCR profiling of over 1400 Arabidopsis transcription factors: Unprecedented sensitivity reveals novel root- and shoot-specific genes
    • doi:10.1111/j.1365-313X.2004.02051.x
    • Czechowski T, Bari RP, Stitt M, Scheible WR, Udvardi M (2004) Real-time PCR profiling of over 1400 Arabidopsis transcription factors: unprecedented sensitivity reveals novel root- and shoot-specific genes. Plant J 38:366-379 doi:10.1111/j.1365-313X.2004.02051.x.
    • (2004) Plant J , vol.38 , pp. 366-379
    • Czechowski, T.1    Bari, R.P.2    Stitt, M.3    Scheible, W.R.4    Udvardi, M.5
  • 8
    • 20444466570 scopus 로고    scopus 로고
    • Proteomic characterization of evolutionarily conserved and variable proteins of Arabidopsis cytosolic ribosomes
    • doi:10.1104/pp.104.053637
    • Chang IF, Szick-Miranda K, Pan SQ, Bailey-Serres J (2005) Proteomic characterization of evolutionarily conserved and variable proteins of Arabidopsis cytosolic ribosomes. Plant Physiol 137:848-862 doi:10.1104/pp.104.053637.
    • (2005) Plant Physiol , vol.137 , pp. 848-862
    • Chang, I.F.1    Szick-Miranda, K.2    Pan, S.Q.3    Bailey-Serres, J.4
  • 10
    • 70350008334 scopus 로고
    • Nucleic acids and proteins in plants
    • Partheier P, Boulter D (eds), Springer, Berlin
    • Dyer TA (1982) Nucleic acids and proteins in plants. In: Partheier P, Boulter D (eds) Encyclopedia of Plant physiology, vol 14b. Springer, Berlin, pp 171-191.
    • (1982) Encyclopedia of Plant Physiology , vol.14 b , pp. 171-191
    • Dyer, T.A.1
  • 11
    • 33748095919 scopus 로고    scopus 로고
    • Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR
    • doi:10.1007/s10529-006-9127-2
    • Fleige S, Walf V, Huch S, Prgomet C, Sehm J, Pfaffl MW (2006) Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR. Biotechnol Lett 28:1601-1613 doi:10.1007/s10529-006-9127-2.
    • (2006) Biotechnol Lett , vol.28 , pp. 1601-1613
    • Fleige, S.1    Walf, V.2    Huch, S.3    Prgomet, C.4    Sehm, J.5    Pfaffl, M.W.6
  • 12
    • 25444435691 scopus 로고    scopus 로고
    • A qualitative assessment of direct-labeled cDNA products prior to microarray analysis
    • doi:10.1186/1471-2164-6-36
    • Grissom SF, Lobenhofer EK, Tucker CJ (2005) A qualitative assessment of direct-labeled cDNA products prior to microarray analysis. BMC Genomics 6:36 doi:10.1186/1471-2164-6-36.
    • (2005) BMC Genomics , vol.6 , pp. 36
    • Grissom, S.F.1    Lobenhofer, E.K.2    Tucker, C.J.3
  • 13
    • 0027161297 scopus 로고
    • Isolation of full-length RNA templates for reverse transcription from tissues rich in RNase and proteoglycans
    • Groppe JC, Morse DE (1993) Isolation of full-length RNA templates for reverse transcription from tissues rich in RNase and proteoglycans. Anal Biochem 210:337-343.
    • (1993) Anal Biochem , vol.210 , pp. 337-343
    • Groppe, J.C.1    Morse, D.E.2
  • 14
    • 0346655258 scopus 로고    scopus 로고
    • Comparison of RiboGreen and 18S rRNA quantitation for normalizing real-time RT-PCR expression analysis
    • Hashimoto JG, Beadles-Bohling AS, Wiren KM (2004) Comparison of RiboGreen and 18S rRNA quantitation for normalizing real-time RT-PCR expression analysis. Biotechniques 36:54-56 58-60.
    • (2004) Biotechniques , vol.36
    • Hashimoto, J.G.1    Beadles-Bohling, A.S.2    Wiren, K.M.3
  • 16
    • 0023605128 scopus 로고
    • Purification and fractionation of poly(A)+RNA
    • doi:10.1016/0076-6879(87)52028-6
    • Jacobson A (1987) Purification and fractionation of poly(A)+RNA. Methods Enzymol 152:254-261 doi:10.1016/0076-6879(87)52028-6.
    • (1987) Methods Enzymol , vol.152 , pp. 254-261
    • Jacobson, A.1
  • 17
    • 2342520656 scopus 로고    scopus 로고
    • Locked nucleic acid (LNA) single nucleotide polymorphism (SNP) genotype analysis and validation using real-time PCR
    • doi:10.1093/nar/gnh046
    • Johnson MP, Haupt LM, Griffiths LR (2004) Locked nucleic acid (LNA) single nucleotide polymorphism (SNP) genotype analysis and validation using real-time PCR. Nucleic Acids Res 32:E55 doi:10.1093/nar/gnh046.
    • (2004) Nucleic Acids Res , vol.32
    • Johnson, M.P.1    Haupt, L.M.2    Griffiths, L.R.3
  • 18
    • 0037986761 scopus 로고    scopus 로고
    • Laser capture microdissection of cells from plant tissues
    • doi:10.1104/pp.102.018127
    • Kerk NM, Ceserani T, Tausta SL, Sussex IM, Nelson TM (2003) Laser capture microdissection of cells from plant tissues. Plant Physiol 132:27-35 doi:10.1104/pp.102.018127.
    • (2003) Plant Physiol , vol.132 , pp. 27-35
    • Kerk, N.M.1    Ceserani, T.2    Tausta, S.L.3    Sussex, I.M.4    Nelson, T.M.5
  • 19
    • 0036499301 scopus 로고    scopus 로고
    • A new quantitative method of real time reverse transcription polymerase chain reaction assay based on simulation of polymerase chain reaction kinetics
    • doi:10.1006/abio.2001.5530
    • Liu WH, Saint DA (2002a) A new quantitative method of real time reverse transcription polymerase chain reaction assay based on simulation of polymerase chain reaction kinetics. Anal Biochem 302:52-59 doi:10.1006/abio.2001.5530.
    • (2002) Anal Biochem , vol.302 , pp. 52-59
    • Liu, W.H.1    Saint, D.A.2
  • 20
    • 0036289137 scopus 로고    scopus 로고
    • Validation of a quantitative method for real time PCR kinetics
    • doi:10.1016/S0006-291X(02)00478-3
    • Liu WH, Saint DA (2002b) Validation of a quantitative method for real time PCR kinetics. Biochem Biophys Res Commun 294:347-353 doi:10.1016/S0006-291X(02)00478-3.
    • (2002) Biochem Biophys Res Commun , vol.294 , pp. 347-353
    • Liu, W.H.1    Saint, D.A.2
  • 21
    • 0035710746 scopus 로고    scopus 로고
    • Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T))
    • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)). Methods Methods 25:402-408.
    • (2001) Methods Methods , vol.25 , pp. 402-408
    • Livak, K.J.1    Schmittgen, T.D.2
  • 22
    • 0014214078 scopus 로고
    • Diversity of RNA components in green plant tissues
    • doi:10.1038/215363a0
    • Loening UE, Ingle J (1967) Diversity of RNA components in green plant tissues. Nature 215:363-367 doi:10.1038/215363a0.
    • (1967) Nature , vol.215 , pp. 363-367
    • Loening, U.E.1    Ingle, J.2
  • 23
    • 0030004766 scopus 로고    scopus 로고
    • Use of UV methods for measurement of protein and nucleic acid concentrations
    • Manchester KL (1996) Use of UV methods for measurement of protein and nucleic acid concentrations. Biotechniques 20:968-970.
    • (1996) Biotechniques , vol.20 , pp. 968-970
    • Manchester, K.L.1
  • 24
    • 0034109932 scopus 로고    scopus 로고
    • Evaluation of RNA isolation methods and reference genes for RT-PCR analyses of rare target RNA
    • doi:10.1515/CCLM.2000.026
    • Mannhalter C, Koizar D, Mitterbauer G (2000) Evaluation of RNA isolation methods and reference genes for RT-PCR analyses of rare target RNA. Clin Chem Lab Med 38:171-177 doi:10.1515/CCLM.2000.026.
    • (2000) Clin Chem Lab Med , vol.38 , pp. 171-177
    • Mannhalter, C.1    Koizar, D.2    Mitterbauer, G.3
  • 25
    • 7244257594 scopus 로고    scopus 로고
    • PerlPrimer: Cross-platform, graphical primer design for standard, bisulphite and real-time PCR
    • doi:10.1093/bioinformatics/bth254
    • Marshall OJ (2004) PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR. Bioinformatics 20:2471-2472 doi:10.1093/bioinformatics/bth254.
    • (2004) Bioinformatics , vol.20 , pp. 2471-2472
    • Marshall, O.J.1
  • 27
    • 0033986177 scopus 로고    scopus 로고
    • A microfluidic system for high-speed reproducible DNA sizing and quantitation
    • doi:10.1002/(SICI)1522-2683(20000101)21:1<128::AID-ELPS128>3.0.CO;2-M
    • Mueller O, Hahnenberger K, Dittmann M, Yee H, Dubrow R, Nagle R, Ilsley D (2000) A microfluidic system for high-speed reproducible DNA sizing and quantitation. Electrophoresis 21:128-134 doi:10.1002/(SICI)1522-2683(20000101)21:1<128::AID-ELPS128>3.0.CO;2-M.
    • (2000) Electrophoresis , vol.21 , pp. 128-134
    • Mueller, O.1    Hahnenberger, K.2    Dittmann, M.3    Yee, H.4    Dubrow, R.5    Nagle, R.6    Ilsley, D.7
  • 28
    • 33748677884 scopus 로고    scopus 로고
    • Error propagation in relative real-time reverse transcription polymerase chain reaction quantification models: The balance between accuracy and precision
    • doi:10.1016/j.ab.2006.06.020
    • Nordgard O, Kvaloy JT, Farmen RK, Heikkila R (2006) Error propagation in relative real-time reverse transcription polymerase chain reaction quantification models: the balance between accuracy and precision. Anal Biochem 356:182-193 doi:10.1016/j.ab.2006.06.020.
    • (2006) Anal Biochem , vol.356 , pp. 182-193
    • Nordgard, O.1    Kvaloy, J.T.2    Farmen, R.K.3    Heikkila, R.4
  • 29
    • 0037248812 scopus 로고    scopus 로고
    • RTPrimerDB: The real-time PCR primer and probe database
    • doi:10.1093/nar/gkg011
    • Pattyn F, Speleman F, De Paepe A, Vandesompele J (2003) RTPrimerDB: the real-time PCR primer and probe database. Nucleic Acids Res 31:122-123 doi:310.1093/nar/gkg011.
    • (2003) Nucleic Acids Res , vol.31 , pp. 122-123
    • Pattyn, F.1    Speleman, F.2    De Paepe, A.3    Vandesompele, J.4
  • 30
    • 17344392308 scopus 로고    scopus 로고
    • A new mathematical model for relative quantification in real-time PCR
    • doi:10.1093/nar/29.9.e45
    • Pfaffl MW (2001) A new mathematical model for relative quantification in real-time PCR. Nucleic Acids Res 29:2002-2007 doi:10.1093/nar/29.9.e45.
    • (2001) Nucleic Acids Res , vol.29 , pp. 2002-2007
    • Pfaffl, M.W.1
  • 31
    • 0036581160 scopus 로고    scopus 로고
    • Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR
    • doi:10.1093/nar/30.9.e36
    • Pfaffl MW, Horgan GW, Dempfle L (2002) Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res 30:e36 doi:10.1093/nar/30.9.e36.
    • (2002) Nucleic Acids Res , vol.30
    • Pfaffl, M.W.1    Horgan, G.W.2    Dempfle, L.3
  • 32
    • 1842483281 scopus 로고    scopus 로고
    • Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations
    • doi:10.1023/B:BILE.0000019559.84305.47
    • Pfaffl MW, Tichopad A, Prgomet C, Neuvians TP (2004) Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations. Biotechnol Lett 26:509-515 doi:10.1023/B:BILE.0000019559.84305.47.
    • (2004) Biotechnol Lett , vol.26 , pp. 509-515
    • Pfaffl, M.W.1    Tichopad, A.2    Prgomet, C.3    Neuvians, T.P.4
  • 33
  • 34
    • 0035057561 scopus 로고    scopus 로고
    • Validation of array-based gene expression profiles by real-time (kinetic) RT-PCR
    • Rajeevan MS, Vernon SD, Taysavang N, Unger ER (2001) Validation of array-based gene expression profiles by real-time (kinetic) RT-PCR. J Mol Diagn 3:26-31.
    • (2001) J Mol Diagn , vol.3 , pp. 26-31
    • Rajeevan, M.S.1    Vernon, S.D.2    Taysavang, N.3    Unger, E.R.4
  • 35
    • 33646695093 scopus 로고    scopus 로고
    • Conifer defence against insects: Microarray gene expression profiling of Sitka spruce (Picea sitchensis) induced by mechanical wounding or feeding by spruce budworms (Choristoneura occidentalis) or white pine weevils (Pissodes strobi) reveals large-scale changes of the host transcriptome
    • doi:10.1111/j.1365-3040.2006.01532.x
    • Ralph SG, Yueh H, Friedmann M, Aeschliman D, Zeznik JA, Nelson CC, Butterfield YSN, Kirkpatrick R, Liu J, Jones SJM, Marra MA, Douglas CJ, Ritland K, Bohlmann J (2006) Conifer defence against insects: microarray gene expression profiling of Sitka spruce (Picea sitchensis) induced by mechanical wounding or feeding by spruce budworms (Choristoneura occidentalis) or white pine weevils (Pissodes strobi) reveals large-scale changes of the host transcriptome. Plant Cell Environ 29:1545-1570 doi:10.1111/j.1365-3040.2006.01532.x.
    • (2006) Plant Cell Environ , vol.29 , pp. 1545-1570
    • Ralph, S.G.1    Yueh, H.2    Friedmann, M.3    Aeschliman, D.4    Zeznik, J.A.5    Nelson, C.C.6    Butterfield, Y.S.N.7    Kirkpatrick, R.8    Liu, J.9    Jones, S.J.M.10    Marra, M.A.11    Douglas, C.J.12    Ritland, K.13    Bohlmann, J.14
  • 36
    • 0037435395 scopus 로고    scopus 로고
    • Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data
    • doi:10.1016/S0304-3940(02)01423-4
    • Ramakers C, Ruijter JM, Deprez RHL, Moorman AFM (2003) Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data. Neurosci Lett 339:62-66 doi:10.1016/S0304-3940(02)01423-4.
    • (2003) Neurosci Lett , vol.339 , pp. 62-66
    • Ramakers, C.1    Ruijter, J.M.2    Deprez, R.H.L.3    Moorman, A.F.M.4
  • 38
    • 16544384956 scopus 로고    scopus 로고
    • Sigmoidal curve-fitting redefines quantitative real-time PCR with the prospective of developing automated high-throughput applications
    • Rutledge RG (2004) Sigmoidal curve-fitting redefines quantitative real-time PCR with the prospective of developing automated high-throughput applications. Nucleic Acids Res 32:e178.
    • (2004) Nucleic Acids Res , vol.32
    • Rutledge, R.G.1
  • 39
    • 4444337502 scopus 로고    scopus 로고
    • Mathematics of quantitative kinetic PCR and the application of standard curves
    • doi:10.1093/nar/gng093
    • Rutledge RG, Cote C (2003) Mathematics of quantitative kinetic PCR and the application of standard curves. Nucleic Acids Res 31:e93 doi:10.1093/nar/gng093.
    • (2003) Nucleic Acids Res , vol.31
    • Rutledge, R.G.1    Cote, C.2
  • 41
    • 1242296843 scopus 로고    scopus 로고
    • Linear-after-the-exponential (LATE)-PCR: An advanced method of asymmetric PCR and its uses in quantitative real-time analysis
    • doi:10.1073/pnas.0305476101
    • Sanchez JA, Pierce KE, Rice JE, Wangh LJ (2004) Linear-after-the-exponential (LATE)-PCR: an advanced method of asymmetric PCR and its uses in quantitative real-time analysis. Proc Natl Acad Sci U S A 101:1933-8 doi:10.1073/pnas.0305476101.
    • (2004) Proc Natl Acad Sci U S A , vol.101 , pp. 1933-8
    • Sanchez, J.A.1    Pierce, K.E.2    Rice, J.E.3    Wangh, L.J.4
  • 43
    • 1542356381 scopus 로고    scopus 로고
    • Standardized determination of real-time PCR efficiency from a single reaction set-up
    • doi:10.1093/nar/gng122
    • Tichopad A, Dilger M, Schwarz G, Pfaffl MW (2003) Standardized determination of real-time PCR efficiency from a single reaction set-up. Nucleic Acids Res 31:e122 doi:10.1093/nar/gng122.
    • (2003) Nucleic Acids Res , vol.31
    • Tichopad, A.1    Dilger, M.2    Schwarz, G.3    Pfaffl, M.W.4
  • 44
    • 0036955404 scopus 로고    scopus 로고
    • Improving quantitative real-time RT-PCR reproducibility by boosting primer-linked amplification efficiency
    • doi:10.1023/A:1021319421153
    • Tichopad A, Dzidic A, Pfaffl MW (2002) Improving quantitative real-time RT-PCR reproducibility by boosting primer-linked amplification efficiency. Biotechnol Lett 24:2053-2056 doi:10.1023/A:1021319421153.
    • (2002) Biotechnol Lett , vol.24 , pp. 2053-2056
    • Tichopad, A.1    Dzidic, A.2    Pfaffl, M.W.3
  • 45
    • 0037109753 scopus 로고    scopus 로고
    • Quantitative real-time reverse transcription polymerase chain reaction: Normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies
    • doi:10.1016/S0003-2697(02)00311-1
    • Tricarico C, Pinzani P, Bianchi S, Paglierani M, Distante V, Pazzagli M, Bustin SA, Orlando C (2002) Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies. Anal Biochem 309:293-300 doi:10.1016/S0003-2697(02)00311-1.
    • (2002) Anal Biochem , vol.309 , pp. 293-300
    • Tricarico, C.1    Pinzani, P.2    Bianchi, S.3    Paglierani, M.4    Distante, V.5    Pazzagli, M.6    Bustin, S.A.7    Orlando, C.8
  • 46
    • 0037129827 scopus 로고    scopus 로고
    • Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
    • research0034
    • Vandesompele J, De Preter K, Pattyn F, Poppe B, van Roy N, De Paepe A, Speleman F (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3:research0034.
    • (2002) Genome Biol , vol.3
    • Vandesompele, J.1    De Preter, K.2    Pattyn, F.3    Poppe, B.4    van Roy, N.5    De Paepe, A.6    Speleman, F.7
  • 47
    • 24944534978 scopus 로고    scopus 로고
    • Real-time PCR for mRNA quantitation
    • doi:10.2144/05391RV01
    • Wong ML, Medrano JF (2005) Real-time PCR for mRNA quantitation. Biotechniques 39:75-85 doi:10.2144/05391RV01.
    • (2005) Biotechniques , vol.39 , pp. 75-85
    • Wong, M.L.1    Medrano, J.F.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.