Genetic dissection of platelet function in health and disease using systems biology

Hematology/Oncology Clinics of North America

Volumn 27, Issue 3, 2013, Pages 443-463

  Bahou, Wadie F ^a  

^a STONY BROOK UNIVERSITY   (United States)

Author keywords

Gene profiling; Hemostasis; Megakaryocytopoiesis; Proteomics; Thrombosis

Indexed keywords

CELL SURFACE PROTEIN; INTERLEUKIN 1BETA; MESSENGER RNA; MICRORNA; PHOSPHOLIPID ANTIBODY; PLASMINOGEN ACTIVATOR INHIBITOR 1; PROTEIN BCL 3; PROTEINASE ACTIVATED RECEPTOR 1; THROMBIN RECEPTOR ACTIVATING PEPTIDE;

ARTERY THROMBOSIS; BLOOD CLOTTING; CARDIOVASCULAR DISEASE; CEREBROVASCULAR DISEASE; CLINICAL FEATURE; GENE EXPRESSION; GENE EXPRESSION PROFILING; GENE INTERACTION; GENETIC ANALYSIS; GENETIC RISK; GENOTYPE; HEMATOPOIETIC STEM CELL; HEPARIN INDUCED THROMBOCYTOPENIA; HERITABILITY; HUMAN; IDIOPATHIC THROMBOCYTOPENIC PURPURA; MEGAKARYOCYTE; MEGAKARYOPOIESIS; MOLECULAR GENETICS; NEOPLASM; NONHUMAN; PATHOGENESIS; PERIPHERAL BLOOD MONONUCLEAR CELL; PHENOTYPE; PLATELET MICROPARTICLE; POLYSOME; PRIORITY JOURNAL; PROGNOSIS; PROTEIN ANALYSIS; PROTEIN SYNTHESIS; PROTEOMICS; QUANTITATIVE TRAIT LOCUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; REVIEW; RNA SPLICING; ROUGH ENDOPLASMIC RETICULUM; SERIAL ANALYSIS OF GENE EXPRESSION; SINGLE NUCLEOTIDE POLYMORPHISM; SURFACE ENHANCED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; SYSTEMS BIOLOGY; THROMBOCYTE ACTIVATION; THROMBOCYTE FUNCTION; THROMBOCYTOPOIESIS; THROMBOCYTOSIS; THROMBOPHILIA; TRANSCRIPTOMICS;

BLOOD PLATELET DISORDERS; BLOOD PLATELETS; HUMANS; PROGNOSIS; SYSTEMS BIOLOGY;

EID: 84878240490     PISSN: 08898588     EISSN: 15581977     Source Type: Journal    
DOI: 10.1016/j.hoc.2013.03.002     Document Type: Review

References (122)

1. Bahou W.F. Disorders of platelets. Genomics and clinical medicine 2006, 221-248. Oxford University Press, Oxford. D. Kumar (Ed.).
2. Denny-Brown D. Recurrent cerebrovascular episodes. Arch Neurol 1960, 2:194-210.
3. Russell R.W. Observations on the retinal blood-vessels in monocular blindness. Lancet 1961, 2:1422-1428.
4. Cella G., Zahavi J., de Haas H.A., et al. Beta-thromboglobulin, platelet production time and platelet function in vascular disease. Br J Haematol 1979, 43:127-136.
5. Feinberg W.M., Bruck D.C., Ring M.E., et al. Hemostatic markers in acute stroke. Stroke 1989, 20:592-597.
6. Feinberg W.M., Pearce L.A., Hart R.G., et al. Markers of thrombin and platelet activity in patients with atrial fibrillation: correlation with stroke among 1531 participants in the stroke prevention in atrial fibrillation III study. Stroke 1999, 30:2547-2553.
7. Zeller J.A., Tschoepe D., Kessler C. Circulating platelets show increased activation in patients with acute cerebral ischemia. Thromb Haemost 1999, 81:373-377.
8. Uchiyama S., Takeuchi M., Osawa M., et al. Platelet function tests in thrombotic cerebrovascular disorders. Stroke 1983, 14:511-517.
9. Uchiyama S., Yamazaki M., Hara Y., et al. Alterations of platelet, coagulation, and fibrinolysis markers in patients with acute ischemic stroke. Semin Thromb Hemost 1997, 23:535-541.
10. Konstantopoulos K., Grotta J.C., Sills C., et al. Shear-induced platelet aggregation in normal subjects and stroke patients. Thromb Haemost 1995, 74:1329-1334.
11. Collaborative meta-analysis of randomised trials of antiplatelet therapy for prevention of death, myocardial infarction, and stroke in high risk patients. BMJ 2002, 324:71-86. Antithrombotic Trialists' Collaboration.
12. Bousser M.G., Eschwege E., Haguenau M., et al. "AICLA" controlled trial of aspirin and dipyridamole in the secondary prevention of athero-thrombotic cerebral ischemia. Stroke 1983, 14:5-14.
13. The European Stroke Prevention Study (ESPS). Principal end-points. The ESPS Group. Lancet 1987, 2:1351-1354.
14. United Kingdom transient ischaemic attack (UK-TIA) aspirin trial: interim results. UK-TIA Study Group. Br Med J (Clin Res Ed) 1988, 296:316-320.
15. Diener H.C., Cunha L., Forbes C., et al. European Stroke Prevention Study. 2. Dipyridamole and acetylsalicylic acid in the secondary prevention of stroke. J Neurol Sci 1996, 143:1-13.
16. Levine G., Ali M., Schafer A. Antithrombotic therapy in patients with acute coronary syndromes. Arch Intern Med 2001, 161:937-948.
17. del Zoppo G.J. The role of platelets in ischemic stroke. Neurology 1998, 51:S9-S14.
18. McKee S.A., Sane D.C., Deliargyris E.N. Aspirin resistance in cardiovascular disease: a review of prevalence, mechanisms, and clinical significance. Thromb Haemost 2002, 88:711-715.
19. Federman D.G., Kirsner R.S. An update on hypercoagulable disorders. Arch Intern Med 2001, 161:1051-1056.
20. Kunicki T.J., Orchekowski R., Annis D., et al. Variability of integrin alpha2 beta1 activity on human platelets. Blood 1993, 82:2693-2703.
21. Roest M., Sixma J., Wu Y., et al. Platelet adhesion to collagen in healthy volunteers is influenced by variation of both alpha(2) beta(1) density and von Willebrand factor. Blood 2000, 96:1433-1437.
22. Moshfegh K., Wiullemin W., Redondo M., et al. Association of two silent polymorphisms of platelet glycoprotein Ia/IIa receptor with risk of myocardial infarction: a case-control study. Lancet 1999, 353:351-354.
23. Carlsson L., Santoso S., Spitzer C., et al. The alpha2 gene coding sequence T807/A873 of the platelet collagen receptor integrin alpha2 beta1 might be a genetic risk factor for the development of stroke in younger patients. Blood 1999, 93:3583-3586.
24. Carter A., Catto A., Bamford J., et al. Platelet GPIIIa PIA and GPIb variable number tandem repeat polymorphisms and markers of platelet activation in acute stroke. Arterioscler Thromb Vasc Biol 1998, 18:1124-1131.
25. Baker R.I., Eikelboom J., Lofthouse E., et al. Platelet glycoprotein Ibalpha Kozak polymorphism is associated with an increased risk of ischemic stroke. Blood 2001, 98:36-40.
26. Frank M.B., Reiner A.P., Schwartz S.M., et al. The Kozak sequence polymorphism of platelet glycoprotein Ibalpha and risk of nonfatal myocardial infarction and nonfatal stroke in young women. Blood 2001, 97:875-879.
27. Bottiger C., Kastrati A., Koch W., et al. HPA-1 and HPA-3 polymorphisms of the platelet fibrinogen receptor and coronary artery disease and myocardial infarction. Thromb Haemost 2000, 83:559-562.
28. Durante-Mangoni E., Davies G., Ahmed N., et al. Coronary thrombosis and the platelet glycoprotein IIIA gene PLA2 polymorphism. Thromb Haemost 1998, 80:218-219.
29. Scaglione L., Bergerone S., Gaschino G., et al. Lack of relationship between the P1A1/P1A2 polymorphism of platelet glycoprotein IIIa and premature myocardial infarction. Eur J Clin Invest 1998, 28:385-388.
30. Sperr W., Huber K.C., Roden M., et al. Inherited platelet glycoprotein polymorphisms and a risk for coronary heart disease in young central Europeans. Thromb Res 1998, 90:117-123.
31. Joven J., Simo J., Vilella E., et al. Lipoprotein(a) and the significance of the association between platelet glycoprotein IIIa polymorphisms and the risk of premature myocardial infarction. Atherosclerosis 1998, 140:155-159.
32. Weedon M.N., Lettre G., Freathy R.M., et al. A common variant of HMGA2 is associated with adult and childhood height in the general population. Nat Genet 2007, 39:1245-1250.
33. Dupont A., Fontana P., Bachelot-Loza C., et al. An intronic polymorphism in the PAR-1 gene is associated with platelet receptor density and the response to SFLLRN. Blood 2003, 101:1833-1840.
34. Fontana P., Dupont A., Gandrille S., et al. Adenosine diphosphate-induced platelet aggregation is associated with P2Y12 gene sequence variations in healthy subjects. Circulation 2003, 108:989-995.
35. Hetherington S.L., Singh R.K., Lodwick D., et al. Dimorphism in the P2Y1 ADP receptor gene is associated with increased platelet activation response to ADP. Arterioscler Thromb Vasc Biol 2005, 25:252-257.
36. Panzer S., Hocker L., Koren D. Agonists-induced platelet activation varies considerably in healthy male individuals: studies by flow cytometry. Ann Hematol 2006, 85:121-125.
37. Yee D.L., Sun C.W., Bergeron A.L., et al. Aggregometry detects platelet hyperreactivity in healthy individuals. Blood 2005, 106:2723-2729.
38. Dale G.L. Coated-platelets: an emerging component of the procoagulant response. J Thromb Haemost 2005, 3:2185-2192.
39. Fontana P., Gandrille S., Remones V., et al. Identification of functional polymorphisms of the thromboxane A2 receptor gene in healthy volunteers. Thromb Haemost 2006, 96:356-360.
40. Jones C.I., Garner S.F., Angenent W., et al. Mapping the platelet profile for functional genomic studies and demonstration of the effect size of the GP6 locus. J Thromb Haemost 2007, 5:1756-1765.
41. O'Donnell C.J., Larson M.G., Feng D., et al. Genetic and environmental contributions to platelet aggregation: the Framingham heart study. Circulation 2001, 103:3051-3056.
42. Gaxiola B., Friedl W., Propping P. Epinephrine-induced platelet aggregation. A twin study. Clin Genet 1984, 26:543-548.
43. Bray P.F., Mathias R.A., Faraday N., et al. Heritability of platelet function in families with premature coronary artery disease. J Thromb Haemost 2007, 5:1617-1623.
44. Gnatenko D.V., Dunn J.J., McCorkle S.R., et al. Transcript profiling of human platelets using microarray and serial analysis of gene expression. Blood 2003, 101:2285-2293.
45. McRedmond J.P., Park S.D., Reilly D.F., et al. Integration of proteomics and genomics in platelets: a profile of platelet proteins and platelet-specific genes. Mol Cell Proteomics 2004, 3:133-144.
46. Weyrich A., Dixon D., Pabla R., et al. Signal-dependent translation of a regulatory protein, Bcl-2, in activated human platelets. Proc Natl Acad Sci U S A 1998, 95:5556-5561.
47. Denis M.M., Tolley N.D., Bunting M., et al. Escaping the nuclear confines: signal-dependent pre-mRNA splicing in anucleate platelets. Cell 2005, 122:379-391.
48. Landry P., Plante I., Ouellet D.L., et al. Existence of a microRNA pathway in anucleate platelets. Nat Struct Mol Biol 2009, 16:961-966.
49. Rinder H., Schuster J., Rinder C., et al. Correlation of thrombosis with increased platelet turnover in thrombocytosis. Blood 1998, 91:1288-1294.
50. Fink L., Holschermann H., Kwapiszewska G., et al. Characterization of platelet-specific mRNA by real-time PCR after laser-assisted microdissection. Thromb Haemost 2003, 90:749-756.
51. Kieffer N., Guichard J., Farcet J., et al. Biosynthesis of major platelet proteins in human blood platelets. Eur J Biochem 1987, 164:189-195.
52. Brogren H., Karlsson L., Andersson M., et al. Platelets synthesize large amounts of active plasminogen activator inhibitor 1. Blood 2004, 104:3943-3948.
53. Velculescu V., Zhang L., Vogelstein B., et al. Serial analysis of gene expression. Science 1995, 270:484-487.
54. Zhang L., Zhou W., Velculescu V., et al. Gene expression profiles in normal and cancer cells. Science 1997, 276:1268-1272.
55. Dunn J.J., McCorkle S.R., Praissman L.A., et al. Genomic signature tags (GSTs): a system for profiling genomic DNA. Genome Res 2002, 12:1756-1765.
56. Wang E., Miller L., Ohnmacht G., et al. High-fidelity mRNA amplification for gene profiling. Nat Biotechnol 2000, 18:457-459.
57. Peters D., Kassam A., Feingold E., et al. Comprehensive transcript analysis in small quantities of mRNA by SAGE-Lite. Nucleic Acids Res 1999, 27:39.
58. Wicki A.N., Walz A., Gerber-Huber S.N., et al. Isolation and characterization of human blood platelet mRNA and construction of a cDNA library in lambda gt11. Confirmation of the platelet derivation by identification of GPIb coding mRNA and cloning of a GPIb coding cDNA insert. Thromb Haemost 1989, 61:448-453.
59. Newman P., Gorski J., White G., et al. Enzymatic amplification of platelet-specific messenger RNA using the polymerase chain reaction. J Clin Invest 1988, 82:739-743.
60. Bugert P., Dugrillon A., Gunaydin A., et al. Messenger RNA profiling of human platelets by microarray hybridization. Thromb Haemost 2003, 90:738-748.
61. Mueller A., Dittrich R., Binder H., et al. High dose estrogen treatment increases bone mineral density in male-to-female transsexuals receiving gonadotropin-releasing hormone agonist in the absence of testosterone. Eur J Endocrinol 2005, 153:107-113.
62. Sauer S., Lange B.M., Gobom J., et al. Miniaturization in functional genomics and proteomics. Nat Rev Genet 2005, 6:465-476.
63. Dittrich E., Puttinger H., Schillinger M., et al. Effect of radio contrast media on residual renal function in peritoneal dialysis patients-a prospective study. Nephrol Dial Transplant 2006, 21:1334-1339.
64. Welle S., Bhatt K., Thornton C. Inventory of high-abundance mRNAs in skeletal muscle of normal men. Genome Res 1999, 9:506-513.
65. Bushati N., Cohen S.M. microRNA functions. Annu Rev Cell Dev Biol 2007, 23:175-205.
66. Merkerova M., Belickova M., Bruchova H. Differential expression of microRNAs in hematopoietic cell lineages. Eur J Haematol 2008, 81:304-310.
67. Yendamuri S., Calin G.A. The role of microRNA in human leukemia: a review. Leukemia 2009, 23:1257-1263.
68. Chen C.Z., Li L., Lodish H.F., et al. MicroRNAs modulate hematopoietic lineage differentiation. Science 2004, 303:83-86.
69. Garzon R., Pichiorri F., Palumbo T., et al. MicroRNA fingerprints during human megakaryocytopoiesis. Proc Natl Acad Sci U S A 2006, 103:5078-5083.
70. Zhou B., Wang S., Mayr C., et al. miR-150, a microRNA expressed in mature B and T cells, blocks early B cell development when expressed prematurely. Proc Natl Acad Sci U S A 2007, 104:7080-7085.
71. Lu J., Guo S., Ebert B.L., et al. MicroRNA-mediated control of cell fate in megakaryocyte-erythrocyte progenitors. Dev Cell 2008, 14:843-853.
72. Bruchova H., Merkerova M., Prchal J.T. Aberrant expression of microRNA in polycythemia vera. Haematologica 2008, 93:1009-1016.
73. Bruchova H., Yoon D., Agarwal A.M., et al. Regulated expression of microRNAs in normal and polycythemia vera erythropoiesis. Exp Hematol 2007, 35:1657-1667.
74. Girardot M., Pecquet C., Boukour S., et al. miR-28 is a thrombopoietin receptor targeting microRNA detected in a fraction of myeloproliferative neoplasm patient platelets. Blood 2010, 116:437-445.
75. Kondkar A.A., Bray M.S., Leal S.M., et al. VAMP8/endobrevin is overexpressed in hyperreactive human platelets: suggested role for platelet microRNA. J Thromb Haemost 2010, 8:369-378.
76. Hanash S.M., Neel J.V., Baier L.J., et al. Genetic analysis of thirty-three platelet polypeptides detected in two-dimensional polyacrylamide gels. Am J Hum Genet 1986, 38:352-360.
77. Giometti C.S., Anderson N.G. Protein changes in activated human platelets. Clin Chem 1984, 30:2078-2083.
78. Gravel P., Sanchez J.C., Walzer C., et al. Human blood platelet protein map established by two-dimensional polyacrylamide gel electrophoresis. Electrophoresis 1995, 16:1152-1159.
79. Marcus K., Immler D., Sternberger J., et al. Identification of platelet proteins separated by two-dimensional gel electrophoresis and analyzed by matrix assisted laser desorption/ionization-time of flight-mass spectrometry and detection of tyrosine-phosphorylated proteins. Electrophoresis 2000, 21:2622-2636.
80. Garcia A., Prabhakar S., Brock C.J., et al. Extensive analysis of the human platelet proteome by two-dimensional gel electrophoresis and mass spectrometry. Proteomics 2004, 4:656-668.
81. Gevaert K., Ghesquiere B., Staes A., et al. Reversible labeling of cysteine-containing peptides allows their specific chromatographic isolation for non-gel proteome studies. Proteomics 2004, 4:897-908.
82. Gevaert K., Goethals M., Martens L., et al. Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides. Nat Biotechnol 2003, 21:566-569.
83. Martens L., Van Damme P., Van Damme J., et al. The human platelet proteome mapped by peptide-centric proteomics: a functional protein profile. Proteomics 2005, 5:3193-3204.
84. Moebius J., Zahedi R.P., Lewandrowski U., et al. The human platelet membrane proteome reveals several new potential membrane proteins. Mol Cell Proteomics 2005, 4:1754-1761.
85. Nanda N., Bao M., Lin H., et al. Platelet endothelial aggregation receptor 1 (PEAR1), a novel epidermal growth factor repeat-containing transmembrane receptor, participates in platelet contact-induced activation. J Biol Chem 2005, 280:24680-24689.
86. Lewandrowski U., Wortelkamp S., Lohrig K., et al. Platelet membrane proteomics: a novel repository for functional research. Blood 2009, 114:e10-e19.
87. Coppinger J.A., Cagney G., Toomey S., et al. Characterization of the proteins released from activated platelets leads to localization of novel platelet proteins in human atherosclerotic lesions. Blood 2004, 103:2096-2104.
88. Maynard D.M., Heijnen H.F., Horne M.K., et al. Proteomic analysis of platelet alpha-granules using mass spectrometry. J Thromb Haemost 2007, 5:1945-1955.
89. Hernandez-Ruiz L., Valverde F., Jimenez-Nunez M.D., et al. Organellar proteomics of human platelet dense granules reveals that 14-3-3zeta is a granule protein related to atherosclerosis. J Proteome Res 2007, 6:4449-4457.
90. Lewandrowski U., Sickmann A. N-glycosylation site analysis of human platelet proteins by hydrazide affinity capturing and LC-MS/MS. Methods Mol Biol 2009, 534:225-238.
91. Zahedi R.P., Lewandrowski U., Wiesner J., et al. Phosphoproteome of resting human platelets. J Proteome Res 2008, 7:526-534.
92. Garcia B.A., Smalley D.M., Cho H., et al. The platelet microparticle proteome. J Proteome Res 2005, 4:1516-1521.
93. Gnatenko D.V., Cupit L.D., Huang E.C., et al. Platelets express steroidogenic 17beta-hydroxysteroid dehydrogenases. Distinct profiles predict the essential thrombocythemic phenotype. Thromb Haemost 2005, 94:412-421.
94. Tefferi A., Thiele J., Orazi A., et al. Proposals and rationale for revision of the World Health Organization diagnostic criteria for polycythemia vera, essential thrombocythemia, and primary myelofibrosis: recommendations from an ad hoc international expert panel. Blood 2007, 110:1092-1097.
95. Geissler W.M., Davis D.L., Wu L., et al. Male pseudohermaphroditism caused by mutations of testicular 17 beta-hydroxysteroid dehydrogenase 3. Nat Genet 1994, 7:34-39.
96. Dore L.C., Amigo J.D., Dos Santos C.O., et al. A GATA-1-regulated microRNA locus essential for erythropoiesis. Proc Natl Acad Sci U S A 2008, 105:3333-3338.
97. Moore D.F., Li H., Jeffries N., et al. Using peripheral blood mononuclear cells to determine a gene expression profile of acute ischemic stroke: a pilot investigation. Circulation 2005, 111:212-221.
98. Tang Y., Xu H., Du X., et al. Gene expression in blood changes rapidly in neutrophils and monocytes after ischemic stroke in humans: a microarray study. J Cereb Blood Flow Metab 2006, 26:1089-1102.
99. Ma J., Liew C.C. Gene profiling identifies secreted protein transcripts from peripheral blood cells in coronary artery disease. J Mol Cell Cardiol 2003, 35:993-998.
100. Karra R., Vemullapalli S., Dong C., et al. Molecular evidence for arterial repair in atherosclerosis. Proc Natl Acad Sci U S A 2005, 102:16789-16794.
101. Seo D., Wang T., Dressman H., et al. Gene expression phenotypes of atherosclerosis. Arterioscler Thromb Vasc Biol 2004, 24:1922-1927.
102. Tomic V., Russwurm S., Moller E., et al. Transcriptomic and proteomic patterns of systemic inflammation in on-pump and off-pump coronary artery bypass grafting. Circulation 2005, 112:2912-2920.
103. Seeburger J., Hoffmann J., Wendel H.P., et al. Gene expression changes in leukocytes during cardiopulmonary bypass are dependent on circuit coating. Circulation 2005, 112:I224-I228.
104. Potti A., Bild A., Dressman H.K., et al. Gene-expression patterns predict phenotypes of immune-mediated thrombosis. Blood 2006, 107:1391-1396.
105. Marshall J., Kupchak P., Zhu W., et al. Processing of serum proteins underlies the mass spectral fingerprinting of myocardial infarction. J Proteome Res 2003, 2:361-372.
106. Misek D.E., Kuick R., Wang H., et al. A wide range of protein isoforms in serum and plasma uncovered by a quantitative intact protein analysis system. Proteomics 2005, 5:3343-3352.
107. Svensson A.M., Whiteley G.R., Callas P.W., et al. SELDI-TOF plasma profiles distinguish individuals in a protein C-deficient family with thrombotic episodes occurring before age 40. Thromb Haemost 2006, 96:725-730.
108. Watanabe H., Hamada H., Yamada N., et al. Proteome analysis reveals elevated serum levels of clusterin in patients with preeclampsia. Proteomics 2004, 4:537-543.
109. Heitner J.C., Koy C., Kreutzer M., et al. Differentiation of HELLP patients from healthy pregnant women by proteome analysis-on the way towards a clinical marker set. J Chromatogr B Analyt Technol Biomed Life Sci 2006, 840:10-19.
110. Allard L., Lescuyer P., Burgess J., et al. ApoC-I and ApoC-III as potential plasmatic markers to distinguish between ischemic and hemorrhagic stroke. Proteomics 2004, 4:2242-2251.
111. Zimmermann-Ivol C.G., Burkhard P.R., Le Floch-Rohr J., et al. Fatty acid binding protein as a serum marker for the early diagnosis of stroke: a pilot study. Mol Cell Proteomics 2004, 3:66-72.
112. Mateos-Caceres P.J., Garcia-Mendez A., Lopez-Farre A., et al. Proteomic analysis of plasma from patients during an acute coronary syndrome. J Am Coll Cardiol 2004, 44:1578-1583.
113. Lopez-Farre A.J., Mateos-Caceres P.J., Sacristan D., et al. Relationship between vitamin D binding protein and aspirin resistance in coronary ischemic patients: a proteomic study. J Proteome Res 2007, 6:2481-2487.
114. Italiano J.E., Richardson J.L., Patel-Hett S., et al. Angiogenesis is regulated by a novel mechanism: pro- and antiangiogenic proteins are organized into separate platelet alpha granules and differentially released. Blood 2008, 111:1227-1233.
115. Cervi D., Yip T.T., Bhattacharya N., et al. Platelet-associated PF-4 as a biomarker of early tumor growth. Blood 2008, 111:1201-1207.
116. Klement G.L., Yip T.T., Cassiola F., et al. Platelets actively sequester angiogenesis regulators. Blood 2009, 113:2835-2842.
117. Pietramaggiori G., Scherer S.S., Cervi D., et al. Tumors stimulate platelet delivery of angiogenic factors in vivo: an unexpected benefit. Am J Pathol 2008, 173:1609-1616.
118. Larsson A., Skoldenberg E., Ericson H. Serum and plasma levels of FGF-2 and VEGF in healthy blood donors. Angiogenesis 2002, 5:107-110.
119. Peterson J.E., Zurakowski D., Italiano J.E., et al. Normal ranges of angiogenesis regulatory proteins in human platelets. Am J Hematol 2010, 85:487-493.
120. Gnatenko D.V., Zhu W., Xu X., et al. Class prediction models of thrombocytosis using genetic biomarkers. Blood 2010, 115:7-14.
121. Xu X., Gnatenko D., Ju J., et al. Systematic analysis of microRNA fingerprints in thrombocythemic platelets using integrated platforms. Blood 2012, 120(17):3575-3585.
122. Opalinska J.B., Bersenev A., Zhang Z., et al. MicroRNA expression in maturing murine megakaryocytes. Blood 2010, 116:e128-e138.