Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides

Nature Biotechnology

Volumn 21, Issue 5, 2003, Pages 566-569

  Gevaert, Kris ^a   Goethals, Marc ^a   Martens, Lennart ^a   Van Damme, Jozef ^a   Staes, An ^a   Thomas, Grégoire R ^a   Vandekerckhove, Joël ^a  

^a DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

CHROMATOGRAPHY; ELECTROPHORESIS; ENZYME KINETICS; ESCHERICHIA COLI; MASS SPECTROMETRY;

NON-GEL TECHNIQUES;

PROTEINS;

BACTERIAL PROTEIN; PEPTIDE DERIVATIVE; PROTEOME;

AMINO TERMINAL SEQUENCE; ARTICLE; ESCHERICHIA COLI; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ISOLATION; PROTEIN PROCESSING;

BLOOD PLATELETS; CELL MEMBRANE; CYTOSOL; HUMANS; MASS SPECTROMETRY; PEPTIDES; PROTEOME;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0038333588     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt810     Document Type: Article

References (17)

1. Shen, Y. et al. High-throughput proteomics using high-efficiency multiple-capillary liquid chromatography with on-line high-performance ESI FTICR mass spectrometry. Anal. Chem. 73, 3011-3021 (2001).
2. Washburn, M.P., Wolters, D. & Yates III, J.R. Large-scale analysis of the yeast proteome by multidimensional protein identification technology. Nat. Biotechnol. 19, 242-248 (2001).
3. Gygi, S.P. et al. Quantitative analysis of complex protein mixtures using isotope-coded affinity tags. Nat. Biotechnol. 17, 994-999 (1999).
4. Geng, M., Ji, J. & Regnier, F.E. Signature-peptide approach to detecting proteins in complex mixtures. J. Chromatogr. A. 870, 295-313 (2000).
5. Oda, Y., Nagasu, T. & Chait, B.T. Enrichment analysis of phosphorylated proteins as a tool for probing the phosphoproteome. Nat. Biotechnol. 19, 379-382 (2001).
6. Wang, S. & Regnier, F.E. Proteomics based on selecting and quantifying cysteine-containing peptides by covalent chromatography. J. Chromatogr. A. 924, 345-357 (2001).
7. Zhou, H., Watts, J.D. & Aebersold, R. A systematic approach to the analysis of protein phosphorylation. Nat. Biotechnol. 19, 375-378 (2001).
8. Zhou, H., Ranish, J.A., Watts, J.D. & Aebersold, R. Quantitative proteome analysis by solid-phase isotope tagging and mass spectrometry. Nat. Biotechnol. 20, 512-515 (2002).
9. Brown, J.R. & Hartley, B.S. Location of disulphide bridges by diagonal paper electrophoresis. The disulphide bridges of bovine chymotrypsinogen A. Biochem. J. 101, 214-228 (1966).
10. Cruickshank, W.H., Malchy, B.L. & Kaplan, H. Diagonal chromatography for the selective purification of tyrosyl peptides. Can. J. Biochem. 52, 1013-1017 (1974).
11. Gevaert, K. et al. Chromatographic isolation of methionine-containing peptides for gel-free proteome analysis: Identification of more than 800 Escherichia coli proteins. Mol. Cell. Proteomics 1, 896-903 (2002).
12. Yumoto, N. & Tokushige, M. Acetylation-induced alteration of catalytic and regulatory properties of aspartase. Biochim. Biophys. Acta 749, 101-115 (1983).
13. Okuyama, T. & Satake, K. On the preparation and properties of 2, 4, 6-trinitrophenyl-amino acids and -peptides. J. Biochem. (Tokyo) 47, 454 (1960).
14. Fields, R. The rapid determination of amino groups with TNBS. Methods Enzymol. 25, 464-468 (1972).
15. Fox, J.E. Identification of actin-binding protein as the protein linking the membrane skeleton to glycoproteins on platelet plasma membranes. J. Biol. Chem. 260, 11970-11977 (1985).
16. Perkins, D.N., Pappin, D.J., Creasy, D.M. & Cottrell, J.S. Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis 20, 3551-3567 (1999).
17. O'Connor, P.B. & Costello, C.E. Internal calibration on adjacent samples (InCAS) with Fourier transform mass spectrometry. Anal. Chem. 72, 5881-5885 (2000).