Kinetics and thermal properties of crude and purified β-galactosidase with potential for the production of galactooligosaccharides

Food Technology and Biotechnology

Volumn 51, Issue 1, 2013, Pages 45-52

  Braga, Anna Rafaela Cavalcante ^a   Manera, Ana Paula ^b   Ores, Joana da Costa ^a   Sala, Luisa ^a   Maugeri, Francisco ^c   Kalil, Susana Juliano ^a  

^a FEDERAL UNIVERSITY OF RIO GRANDE   (Brazil)

^b UNIVERSIDADE FEDERAL DO PAMPA   (Brazil)

^c UNIVERSITY OF CAMPINAS   (Brazil)

Author keywords

Inactivation kinetics; Thermal properties; Thermodynamic parameters

Indexed keywords

GALACTO-OLIGOSACCHARIDES; INACTIVATION KINETICS; KINETIC AND THERMODYNAMIC PARAMETERS; KLUYVEROMYCES MARXIANUS; OPTIMUM TEMPERATURE; PURIFICATION PROCESS; PURIFIED ENZYME; THERMODYNAMIC PARAMETER;

DAIRY PRODUCTS; HYDROLYSIS; KINETICS; PURIFICATION; SUGARS; THERMODYNAMIC PROPERTIES;

ENZYMES;

EID: 84877806979     PISSN: 13309862     EISSN: 13342606     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. V. Gekas, M. López-Leiva, Hydrolysis of lactose: A literature review, Process Biochem. 20 (1985) 1-12.
2. A.R.C. Braga, P.A. Gomes, S.J. Kalil, Formulation of culture medium with agroindustrial waste for b-galactosidase production from Kluyveromyces marxianus ATCC 16045, Food Bioprocess Technol. 5 (2012) 1653-1663.
3. R.R. Mahoney, Galactosyl-oligosaccharide formation during lactose hydrolysis: A review, Food Chem. 63 (1998) 147-154.
4. T. Sako, K. Matsumoto, R. Tanaka, Recent progress on research and applications of non-digestible galacto-oligosaccharides, Int. Dairy J. 9 (1999) 69-80.
5. P. Sears, C.H. Wong, Toward automated synthesis of oligosaccharides and glycoproteins, Science, 291 (2001) 2344-2350.
6. M. Ladero, A. Santos, F. García-Ochoa, Kinetic modeling of lactose hydrolysis with an immobilized b-galactosidase from Kluyveromyces fragilis, Enzyme Microbial Technol. 27 (2000) 583-592.
7. J. Szczodrak, Hydrolysis of lactose in whey permeate by immobilized b-galactosidase from Kluyveromyces fragilis, J. Mol. Catal. B: Enzym. 10 (2000) 631-637.
8. E. Jurado, F. Camacho, G. Luzon, J.M. Vicaria, Kinetic models of activity for b-galactosidases: Influence of pH, ionic concentration and temperature, Enzyme Microbial Technol. 34 (2004) 33-40.
9. F.I. Ustok, C. Tari, S. Harsa, Biochemical and thermal properties of b-galactosidase enzymes produced by artisanal yoghurt cultures, Food Chem. 119 (2010) 1114-1120.
10. A.P. Manera, J.C. Ores, V.A. Ribeiro, C.A.V. Burkert, S.J. Kalil, Optimization of the culture medium for the production of b-galactosidase from Kluyveromyces marxianus CCT 7082, Food Technol. Biotechnol. 46 (2008) 66-72.
11. R. Pinheiro, I. Belo, M. Mota, Growth and b-galactosidase activity in cultures of Kluyveromyces marxianus under increased air pressure, Lett. Appl. Microbiol. 37 (2003) 438-442.
12. T. Lukondeh, N.J. Ashbolt, P.L. Rogers, Fed-batch fermentation for production of Kluyveromyces marxianus FII 510700 cultivated on a lactose-based medium, J. Ind. Microbiol. Biotechnol. 32 (2005) 284-288.
13. F.O. de Medeiros, C.A.V. Burkert, S.J. Kalil, Purification of b-galactosidase by ion exchange chromatography: A study of the elution using an experimental design, Chem. Eng. Technol. 35 (2012) 911-918.
14. F.O. de Medeiros, F.G. Alves, C.R. Lisboa, D. de Souza Martins, C.A.V. Burkert, S.J. Kalil, Ultrasonic waves and glass pearls: A new method of extraction of b-galactosidase for use in laboratory, Quimica Nova, 31 (2008) 336-339 (in Portuguese).
15. J.R. Whitaker: Principles of Enzymology for the Food Sciences, Marcel Dekker, New York, NY, USA (1994).
16. V.A. Inchaurrondo, O.M. Yantorno, C.E. Voget, Yeast growth and b-galactosidase production during aerobic batch cultures in lactose-limited synthetic medium, Process Biochem. 29 (1994) 47-54.
17. A.P. Manera, F.A.A. Costa, M.I. Rodrigues, S.J. Kalil, F. Maugeri Filho, Galacto-oligosaccharides production using permeabilized cells of Kluyveromyces marxianus, Int. J. Food Eng. 6 (2010) 1-15.
18. D. Brady, R. Marchant, L. McHale, A.P. McHale, Isolation and partial characterization of b-galactosidase activity produced by a thermotolerant strain of Kluyveromyces marxianus during growth on lactose-containing media, Enzyme Microbial Technol. 17 (1995) 696-699.
19. L.S. Lai, P.C. Chang, C.T. Chang, Isolation and characterization of superoxide dismutase from wheat seedlings, J. Agric. Food Chem. 56 (2008) 8121-8129.
20. A.R. Alcántara, I. Borreguero, M.T. López-Belmonte, J.V. Sinisterra: Covalent Immobilization of Crude and Partially-Purified Lipases onto Inorganic Supports: Stability and Hyperactivation. In: Progress in Biotechnology, A. Ballesteros, F.J. Plou, J.L. Iborra, P.J. Hailing (Eds.), Elsevier Science B.V., Amsterdam, The Netherlands (1998) pp. 571-576.
21. G.S.N. Naidu, T. Panda, Studies on pH and thermal inactivation of pectolytic enzymes from Aspergillus niger, Biochem. Eng. J. 16 (2003) 57-67.
22. N. Ortega, S. de Diego, M. Perez-Mateos, M.D. Busto, Kinetic properties and thermal behaviour of polygalacturonase used in fruit juice clarification, Food Chem. 88 (2004) 209-217.
23. C.N. Pace, Contribution of the hydrophobic effect to globular protein stability, J. Mol. Biol. 226 (1992) 29-35.
24. M.D. Busto, R.K. Owusu Apenten, D.S. Robinson, Z. Wu, R. Casey, R.K. Hughes, Kinetics of thermal inactivation of pea seed lipoxygenases and the effect of additives on their thermostability, Food Chem. 65 (1999) 323-329.
25. E.D. Brown, R.Y. Yada, A kinetic and equilibrium study of the denaturation of aspartic proteinases from fungi, Endothia parasitica and Mucor miehei, Biochim. Biophys. Acta, 1076 (1991) 406-415.
26. E. Cobos, P. Estrada, Effect of polyhydroxylic cosolvents on the thermostability and activity of xylanase from Trichoderma reesei QM 9414, Enzyme Microbial Technol. 33 (2003) 810-818.
27. M.L. Shuler, F. Kargi: Bioprocess Engineering: Basic Concepts, Prentice Hall, Upper Saddle River, NJ, USA (2002).
28. Q.Z.K. Zhou, X.D. Chen, Effects of temperature and pH on the catalytic activity of the immobilized b-galactosidase from Kluyveromyces lactis, Biochem. Eng. J. 9 (2001) 33-44.
29. Z. Nagy, T. Kiss, A. Szentirmai, S. Biro, b-Galactosidase of Penicillium chrysogenum: Production, purification, and characterization of the enzyme, Protein Expres. Purif. 21 (2001) 24-29.