Improvement of natamycin production by engineering of phosphopantetheinyl transferases in Streptomyces chattanoogensis L10

Applied and Environmental Microbiology

Volumn 79, Issue 11, 2013, Pages 3346-3354

  Jiang, Hui ^a,c   Wang, Yue Yue ^a   Ran, Xin Xin ^a   Fan, Wei Ming ^b   Jiang, Xin Hang ^a   Guan, Wen Jun ^a,c   Li, Yong Quan ^a,c  

^a ZHEJIANG UNIVERSITY   (China)

^b Zhejiang Zhenyuan Pharmaceutical Co Ltd   (China)

^c Key Laboratory of Microbial Biochemistry and Metabolism Engineering of Zhejiang Province   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ACYL CARRIER PROTEINS; BIOCHEMICAL CHARACTERIZATION; NONRIBOSOMAL PEPTIDE; NONRIBOSOMAL PEPTIDE SYNTHETASES; OVER-EXPRESSION; PEPTIDYL CARRIER PROTEINS; POLYKETIDE SYNTHASES; PRODUCTION OF;

BIOCHEMISTRY; CATALYSIS; ENZYMES; KETONES; PROTEINS;

BACTERIA;

BACTERIAL PROTEIN; NATAMYCIN; PHOSPHOPANTETHEINYL TRANSFERASE; PHOSPHOTRANSFERASE;

BIOCHEMISTRY; BIOTECHNOLOGY; ENZYME; PIGMENT;

AMINO ACID SEQUENCE; ARTICLE; BIOENGINEERING; BIOREACTOR; BIOSYNTHESIS; DNA SEQUENCE; ENZYMOLOGY; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; SEQUENCE ALIGNMENT; STREPTOMYCES;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; BIOENGINEERING; BIOREACTORS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; NATAMYCIN; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; STREPTOMYCES; TRANSFERASES (OTHER SUBSTITUTED PHOSPHATE GROUPS);

EID: 84877147429     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00099-13     Document Type: Article

References (39)

1. Lambalot RH, Gehring AM, Flugel RS, Zuber P, LaCelle M, Marahiel MA, Reid R, Khosla C, Walsh CT. 1996. A new enzyme superfamily-the phosphopantetheinyl transferases. Chem. Biol. 3:923-936.
2. Walsh CT, Gehring AM, Weinreb PH, Quadri LE, Flugel RS. 1997. Post-translational modification of polyketide and nonribosomal peptide synthases. Curr. Opin. Chem. Biol. 1:309-315.
3. Barekzi N, Joshi S, Irwin S, Ontl T, Schweizer HP. 2004. Genetic characterization of pcpS, encoding the multifunctional phosphopantetheinyl transferase of Pseudomonas aeruginosa. Microbiology 150:795-803.
4. Finking R, Solsbacher J, Konz D, Schobert M, Schafer A, Jahn D, Marahiel MA. 2002. Characterization of a new type of phosphopantetheinyl transferase for fatty acid and siderophore synthesis in Pseudomonas aeruginosa. J. Biol. Chem. 277:50293-50302.
5. Lu YW, San Roman AK, Gehring AM. 2008. Role of phosphopantetheinyl transferase genes in antibiotic production by Streptomyces coelicolor. J. Bacteriol. 190:6903-6908.
6. Huang Y, Wendt-Pienkowski E, Shen B. 2006. A dedicated phosphopantetheinyl transferase for the fredericamycin polyketide synthase from Streptomyces griseus. J. Biol. Chem. 281:29660-29668.
7. Gomez-Escribano JP, Bibb MJ. 2011. Engineering Streptomyces coelicolor for heterologous expression of secondary metabolite gene clusters. Microb. Biotechnol. 4:207-215.
8. Meiser P, Muller R. 2008. Two functionally redundant Sfp-type 4=-phosphopantetheinyl transferases differentially activate biosynthetic pathways in Myxococcus xanthus. Chembiochem 9:1549-1553.
9. Weissman KJ, Hong H, Oliynyk M, Siskos AP, Leadlay PF. 2004. Identification of a phosphopantetheinyl transferase for erythromycin biosynthesis in Saccharopolyspora erythraea. Chembiochem 5:116-125.
10. Sanchez C, Du L, Edwards DJ, Toney MD, Shen B. 2001. Cloning and characterization of a phosphopantetheinyl transferase from Streptomyces verticillus ATCC15003, the producer of the hybrid peptide-polyketide antitumor drug bleomycin. Chem. Biol. 8:725-738.
11. Murugan E, Liang ZX. 2008. Evidence for a novel phosphopantetheinyl transferase domain in the polyketide synthase for enediyne biosynthesis. FEBS Lett. 582:1097-1103.
12. el-Enshasy HA, Farid MA, el-Sayed SA. 2000. Influence of inoculum type and cultivation conditions on natamycin production by Streptomyces natalensis. J. Basic Microbiol. 40:333-342.
13. Chen GQ, Lu FP, Du LX. 2008. Natamycin production by Streptomyces gilvosporeus based on statistical optimization. J. Agric. Food Chem. 56: 5057-5061.
14. Du YL, Chen SF, Cheng LY, Shen XL, Tian Y, Li YQ. 2009. Identification of a novel Streptomyces chattanoogensis L10 and enhancing its natamycin production by overexpressing positive regulator ScnRII. J. Microbiol. 47:506-513.
15. Anton N, Santos-Aberturas J, Mendes MV, Guerra SM, Martin JF, Aparicio JF. 2007. PimM, a PAS domain positive regulator of pimaricin biosynthesis in Streptomyces natalensis. Microbiology 153:3174-3183.
16. Anton N, Mendes MV, Martin JF, Aparicio JF. 2004. Identification of PimR as a positive regulator of pimaricin biosynthesis in Streptomyces natalensis. J. Bacteriol. 186:2567-2575.
17. Lee KM, Lee CK, Choi SU, Park HR, Kitani S, Nihira T, Hwang YI. 2005. Cloning and in vivo functional analysis by disruption of a gene encoding the gamma-butyrolactone autoregulator receptor from Streptomyces natalensis. Arch. Microbiol. 184:249-257.
18. Du YL, Shen XL, Yu P, Bai LQ, Li YQ. 2011. Gamma-butyrolactone regulatory system of Streptomyces chattanoogensis links nutrient utiliza-tion, metabolism, and development. Appl. Environ. Microbiol. 77:8415-8426.
19. Recio E, Aparicio JF, Rumbero A, Martin JF. 2006. Glycerol, ethylene glycol and propanediol elicit pimaricin biosynthesis in the PI-factordefective strain Streptomyces natalensis npi287 and increase polyene production in several wild-type actinomycetes. Microbiology 152:3147-3156.
20. Recio E, Colinas A, Rumbero A, Aparicio JF, Martin JF. 2004. PI factor, a novel type quorum-sensing inducer elicits pimaricin production in Streptomyces natalensis. J. Biol. Chem. 279:41586-41593.
21. Mendes MV, Recio E, Fouces R, Luiten R, Martin JF, Aparicio JF. 2001. Engineered biosynthesis of novel polyenes: a pimaricin derivative produced by targeted gene disruption in Streptomyces natalensis. Chem. Biol. 8:635-644.
22. Mendes MV, Tunca S, Anton N, Recio E, Sola-Landa A, Aparicio JF, Martin JF. 2007. The two-component phoR-phoP system of Streptomyces natalensis: inactivation or deletion of phoP reduces the negative phosphate regulation of pimaricin biosynthesis. Metab. Eng. 9:217-227.
23. Du YL, Li SZ, Zhou Z, Chen SF, Fan WM, Li YQ. 2011. The pleitropic regulator AdpAch is required for natamycin biosynthesis and morphological differentiation in Streptomyces chattanoogensis. Microbiology 157: 1300-1311.
24. Vicente CM, Santos-Aberturas J, Guerra SM, Payero TD, Martin JF, Aparicio JF. 2009. PimT, an amino acid exporter controls polyene production via secretion of the quorum sensing pimaricin-inducer PI-factor in Streptomyces natalensis. Microb. Cell Fact. 8:33. doi:10.1186/1475-2859-8-33.
25. Kieser T, Bibb MJ, Buttner MJ, Chater KF, Hopwood DA. 2000. Practical Streptomyces genetics. The John Innes Foundation, Norwich, United Kingdom.
26. Margulies M, Egholm M, Altman WE, Attiya S, Bader JS, Bemben LA, Berka J, Braverman MS, Chen YJ, Chen Z, Dewell SB, Du L, Fierro JM, Gomes XV, Godwin BC, He W, Helgesen S, Ho CH, Irzyk GP, Jando SC, Alenquer ML, Jarvie TP, Jirage KB, Kim JB, Knight JR, Lanza JR, Leamon JH, Lefkowitz SM, Lei M, Li J, Lohman KL, Lu H, Makhijani VB, McDade KE, McKenna MP, Myers EW, Nickerson E, Nobile JR, Plant R, Puc BP, Ronan MT, Roth GT, Sarkis GJ, Simons JF, Simpson JW, Srinivasan M, Tartaro KR, Tomasz A, Vogt KA, Volkmer GA, Wang SH, Wang Y, Weiner MP, Yu P, Begley RF, Rothberg JM. 2005. Genome sequencing in microfabricated high-density picolitre reactors. Nature 437:376-380.
27. Delcher AL, Harmon D, Kasif S, White O, Salzberg SL. 1999. Improved microbial gene identification with GLIMMER. Nucleic Acids Res. 27: 4636-4641.
28. Guo FB, Ou HY, Zhang CT. 2003. ZCURVE: a new system for recognizing protein-coding genes in bacterial and archaeal genomes. Nucleic Acids Res. 31:1780-1789.
29. Tatusov RL, Galperin MY, Natale DA, Koonin EV. 2000. The COG database: a tool for genome-scale analysis of protein functions and evolution. Nucleic Acids Res. 28:33-36.
30. Marchler-Bauer A, Anderson JB, Derbyshire MK, DeWeese-Scott C, Gonzales NR, Gwadz M, Hao L, He S, Hurwitz DI, Jackson JD, Ke Z, Krylov D, Lanczycki CJ, Liebert CA, Liu C, Lu F, Lu S, Marchler GH, Mullokandov M, Song JS, Thanki N, Yamashita RA, Yin JJ, Zhang D, Bryant SH. 2007. CDD: a conserved domain database for interactive domain family analysis. Nucleic Acids Res. 35:D237-D240. doi:10.1093/nar /gkl951.
31. Gust B, Challis GL, Fowler K, Kieser T, Chater KF. 2003. PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin. Proc. Natl. Acad. Sci. U. S. A. 100:1541-1546.
32. Sun J, Kelemen GH, Fernandez-Abalos JM, Bibb MJ. 1999. Green fluorescent protein as a reporter for spatial and temporal gene expression in Streptomyces coelicolor A3(2). Microbiology 145(Part 9):2221-2227.
33. Blanco G, Pereda A, Brian P, Mendez C, Chater KF, Salas JA. 1993. A hydroxylase-like gene product contributes to synthesis of a polyketide spore pigment in Streptomyces halstedii. J. Bacteriol. 175:8043-8048.
34. Kelemen GH, Brian P, Flardh K, Chamberlin L, Chater KF, Buttner MJ. 1998. Developmental regulation of transcription of whiE, a locus specifying the polyketide spore pigment in Streptomyces coelicolor A3(2). J. Bacteriol. 180:2515-2521.
35. Novakova R, Bistakova J, Kormanec J. 2004. Characterization of the polyketide spore pigment cluster whiESa in Streptomyces aureofaciens CCM3239. Arch. Microbiol. 182:388-395.
36. Yu TW, Hopwood DA. 1995. Ectopic expression of the Streptomyces coelicolor whiE genes for polyketide spore pigment synthesis and their interaction with the act genes for actinorhodin biosynthesis. Microbiology 141(Part 11):2779-2791.
37. Yu TW, Shen Y, McDaniel R, Floss HG, Khosla C, Hopwood DA, Moore BS. 1998. Engineered biosynthesis of novel polyketides from Streptomyces spore pigment polyketide synthases. J. Am. Chem. Soc. 120:7749-7759.
38. Lim SK, Ju J, Zazopoulos E, Jiang H, Seo JW, Chen Y, Feng Z, Rajski SR, Farnet CM, Shen B. 2009. iso-Migrastatin, migrastatin, and dorrigocin production in Streptomyces platensis NRRL 18993 is governed by a single biosynthetic machinery featuring an acyltransferase-less type I polyketide synthase. J. Biol. Chem. 284:29746-29756.
39. Reuter K, Mofid MR, Marahiel MA, Ficner R. 1999. Crystal structure of the surfactin synthetase-activating enzyme sfp: a prototype of the 4=-phosphopantetheinyl transferase superfamily. EMBO J. 18:6823-6831.