Capillary electrophoresis applied to screening of trypsin inhibitors using microreactor with trypsin immobilized by glutaraldehyde

Analytical Biochemistry

Volumn 438, Issue 1, 2013, Pages 32-38

  Min, Wenao ^a   Cui, Shumin ^a   Wang, Weiping ^a   Chen, Jianrong ^a   Hu, Zhide ^b  

^a ZHEJIANG NORMAL UNIVERSITY   (China)

^b LANZHOU UNIVERSITY   (China)

Author keywords

Capillary electrophoresis; Immobilized enzyme microreactor; Inhibitor screening; Natural extract; Trypsin

Indexed keywords

CAPILLARY ELECTROPHORESIS; ENZYME KINETICS; SEPARATION;

IMMOBILIZED ENZYME; INHIBITION KINETICS; INHIBITOR SCREENINGS; INHIBITORY CONCENTRATION; NATURAL EXTRACTS; OPTIMIZED CONDITIONS; SEPARATION EFFICIENCY; TRYPSIN;

CHEMICAL REACTORS;

BENZAMIDINE DERIVATIVE; GLUTARALDEHYDE; IMMOBILIZED ENZYME; TRYPSIN; TRYPSIN INHIBITOR;

ADSORPTION; ANIMAL TISSUE; ARTICLE; BUPLEURUM; CAPILLARY; CAPILLARY ELECTROPHORESIS; CAPILLARY WALL; COLOCYNTH; CONTROLLED STUDY; COW; CROSS LINKING; DRUG SCREENING; ELECTRIC POTENTIAL; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME INACTIVATION; ENZYME KINETICS; ENZYME MECHANISM; EXTRACT; HIGH THROUGHPUT SCREENING; INCUBATION TEMPERATURE; INCUBATION TIME; INHIBITION KINETICS; MICHAELIS CONSTANT; MUNG BEAN; NONHUMAN; PEANUT; PH; PHASEOLUS VULGARIS; PLANT SEED; PRIORITY JOURNAL; SOYBEAN;

EID: 84877056626     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2013.03.010     Document Type: Article

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