High temporal resolution monitoring of enzyme reaction and inhibition using optically gated vacancy capillary electrophoresis and immobilized enzyme

Analytica Chimica Acta

Volumn 683, Issue 1, 2010, Pages 136-142

  Yang, Li ^a   Chen, Chunyang ^a   Chen, Yuanfang ^a   Shi, Jing ^a   Liu, Sidong ^a   Guo, Liping ^a   Xu, Haifeng ^b  

^a NORTHEAST NORMAL UNIVERSITY   (China)

^b JILIN UNIVERSITY   (China)

Author keywords

Capillary electrophoresis; Enzyme assay; Immobilized enzyme; Optically gated vacancy; Temporal resolution monitoring

Indexed keywords

CARBOXYFLUORESCEIN; CLEAVAGE REACTION; ELECTROOSMOTIC FLOW; ENZYMATIC REACTION; ENZYME ASSAY; ENZYME ASSAYS; ENZYME REACTION; FAST REACTION; HIGH TEMPORAL RESOLUTION; IMMOBILIZED ENZYME; IMMOBILIZED TRYPSIN; INHIBITION CONSTANTS; LASER INDUCED FLUORESCENCE DETECTION; MICHAELIS CONSTANTS; NOVEL METHODS; OPTICALLY GATED VACANCY; PRODUCT FORMATION; REACTION TIME; SUBSTRATE SYSTEM; TEMPORAL RESOLUTION;

BLOOD VESSELS; CAPILLARY ELECTROPHORESIS; ELECTROCHEMISTRY; ELECTROOSMOSIS; ENZYME ACTIVITY; ENZYMES; FLUORESCENCE; REACTION RATES; SUBSTRATES;

ENZYME INHIBITION;

ANGIOTENSIN; CARBOXYFLUORESCEIN; TRYPSIN;

ACCURACY; ARTICLE; CAPILLARY ELECTROPHORESIS; CONTROLLED STUDY; ELECTROOSMOSIS; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME IMMOBILIZATION; ENZYME INHIBITION; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SUBSTRATE; FLUORESCENCE ANALYSIS; MICHAELIS CONSTANT; PRIORITY JOURNAL; PROTEIN CLEAVAGE; REACTION TIME; REPRODUCIBILITY; SENSITIVITY ANALYSIS;

ANGIOTENSINS; ELECTROPHORESIS, CAPILLARY; ENZYME ASSAYS; ENZYME INHIBITORS; ENZYMES, IMMOBILIZED; FLUORESCENCE; KINETICS; LASERS; TRYPSIN;

EID: 78649317336     PISSN: 00032670     EISSN: 18734324     Source Type: Journal    
DOI: 10.1016/j.aca.2010.10.013     Document Type: Article

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