Development of a liquid chromatography-based screening methodology for proteolytic enzyme activity

Journal of Chromatography A

Volumn 1216, Issue 20, 2009, Pages 4407-4415

  Schebb, Nils Helge ^a   Vielhaber, Torsten ^a   Jousset, Alexandre ^b   Karst, Uwe ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

^b DARMSTADT UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

HPLC with biochemical detection (BCD); Ion exchange chromatography (IEX); MS multi substrate assay; Protease; Size exclusion chromatography (SEC)

Indexed keywords

HPLC WITH BIOCHEMICAL DETECTION (BCD); ION-EXCHANGE CHROMATOGRAPHY (IEX); MS MULTI-SUBSTRATE ASSAY; PROTEASE; SIZE-EXCLUSION CHROMATOGRAPHY (SEC);

AMINES; AMINO ACIDS; BODY FLUIDS; CHROMATOGRAPHIC ANALYSIS; COAGULATION; ENZYME ACTIVITY; ENZYMES; HIGH PRESSURE LIQUID CHROMATOGRAPHY; ION CHROMATOGRAPHY; ION EXCHANGE; ION EXCHANGERS; IONS; LIQUIDS; MASS SPECTROMETRY; NITROGEN COMPOUNDS; SIZE EXCLUSION CHROMATOGRAPHY; SIZE SEPARATION; SUBSTRATES; SUGAR (SUCROSE);

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

4 NITROANILINE; CHYMOTRYPSIN; PEPTIDE; SERINE PROTEINASE; TRYPSIN; VENOM;

ACANTHAMOEBA CASTELLANII; ARTICLE; BLOOD CLOTTING; CONTINUOUS FLOW REACTOR; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ISOLATION; GEL PERMEATION CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; SNAKE; TANDEM MASS SPECTROMETRY;

ACANTHAMOEBA CASTELLANII; ANIMALS; BOTHROPS; CHROMATOGRAPHY, LIQUID; PROTOZOAN PROTEINS; SERINE ENDOPEPTIDASES; VIPER VENOMS;

ACANTHAMOEBA CASTELLANII; BOTHROPS MOOJENI; CROTALINAE;

EID: 64649103868     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2009.03.053     Document Type: Article

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