Immobilization of trypsin onto 1,4-diisothiocyanatobenzene-activated porous glass for microreactor-based peptide mapping by capillary electrophoresis: Effect of calcium ions on the immobilization procedure

Analytica Chimica Acta

Volumn 663, Issue 2, 2010, Pages 198-205

  Dartiguenave, Catherine ^a   Hamad, Hussein ^a   Waldron, Karen C ^a  

^a UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

Casein; Capillary electrophoresis; Controlled pore glass; Immobilized enzyme reactor; Peptide mapping; Trypsin

Indexed keywords

CALCIUM IONS; CONTROLLED PORE GLASS; DIGESTION EFFICIENCY; ENZYMATIC ACTIVITIES; IMMOBILIZATION CONDITIONS; IMMOBILIZATION REACTION; IMMOBILIZED ENZYME; IMMOBILIZED ENZYME REACTORS; KINETIC BEHAVIOUR; MICRO-REACTOR; MICRO-SCALES; PEPTIDE MAPPING; POROUS GLASS; PROTEIN HYDROLYSIS; RESIDENCE TIME; SYNTHETIC SUBSTRATES;

CALCIUM; CAPILLARY ELECTROPHORESIS; CASEIN; ELECTROCHEMISTRY; ENZYMES; GLASS; HYDROLYSIS; IONS; MAPPING; PEPTIDES; REUSABILITY;

ENZYME IMMOBILIZATION;

1,4 DIISOTHIOCYANATOBENZENE; BENZENE DERIVATIVE; BETA CASEIN; CALCIUM ION; TRYPSIN; UNCLASSIFIED DRUG;

ARTICLE; CAPILLARY ELECTROPHORESIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME SUBSTRATE; PEPTIDE MAPPING; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN HYDROLYSIS;

BIOREACTORS; CALCIUM; ELECTROPHORESIS, CAPILLARY; ENZYMES, IMMOBILIZED; GLASS; HYDROLYSIS; IONS; ISOTHIOCYANATES; KINETICS; PARTICLE SIZE; PEPTIDE MAPPING; PEPTIDES; POROSITY; PROTEINS; SURFACE PROPERTIES; TRYPSIN;

EID: 77951645792     PISSN: 00032670     EISSN: 18734324     Source Type: Journal    
DOI: 10.1016/j.aca.2010.01.042     Document Type: Article

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