Integrated platform of capillary isoelectric focusing, trypsin immobilized enzyme microreactor and nanoreversed-phase liquid chromatography with mass spectrometry for online protein profiling

Electrophoresis

Volumn 32, Issue 20, 2011, Pages 2848-2856

  Wang, Tingting ^a,b,c   Ma, Junfeng ^a,b,d   Wu, Shuaibin ^a,b   Yuan, Huiming ^a   Zhang, Lihua ^a   Liang, Zhen ^a   Zhang, Yukui ^a  

^a DALIAN INSTITUTE OF CHEMICAL PHYSICS   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c NINGBO UNIVERSITY OF TECHNOLOGY   (China)

^d JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Capillary isoelectric focusing; Immobilized enzyme microreactor; Integrated platform; Nanoreversed phase liquid chromatography; Trypsin

Indexed keywords

CHEMICAL REACTORS; ELECTROPHORESIS; ENZYMES; ESCHERICHIA COLI; ETHYLENE; LIQUID CHROMATOGRAPHY; SILICA;

CAPILLARY ISOELECTRIC FOCUSING; ENZYME MICRO REACTORS; IMMOBILIZED ENZYME; IMMOBILIZED ENZYME MICROREACTOR; INTEGRATED PLATFORM; NANO-REVERSED PHASE LIQUID CHROMATOGRAPHIES; PROTEIN DIGESTION; PROTEIN PROFILING; PROTEIN SEPARATIONS; TRYPSIN;

MASS SPECTROMETRY;

AMPHOLYTE; BACTERIAL PROTEIN; BETA LACTOGLOBULIN; BOVINE SERUM ALBUMIN; MYOGLOBIN; RIBONUCLEASE A;

AMINO ACID SEQUENCE; ANALYTIC METHOD; ARTICLE; ELECTROSPRAY; ESCHERICHIA COLI; ISOELECTRIC FOCUSING; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NANOREVERSED PHASE LIQUID CHROMATOGRAPHY; PROTEIN ANALYSIS; SEPARATION TECHNIQUE; TRYPSIN IMMOBILIZED ENZYME MICROREACTOR;

EID: 80054835043     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.201100030     Document Type: Article

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