Reconstitution of the Anti-Apoptotic Bcl-2 Protein into Lipid Membranes and Biophysical Evidence for Its Detergent-Driven Association with the Pro-Apoptotic Bax Protein

PLoS ONE

Volumn 8, Issue 4, 2013, Pages

  Wallgren, Marcus ^a   Lidman, Martin ^a   Pedersen, Anders ^b   Brännström, Kristoffer ^a   Karlsson, B Göran ^b   Gröbner, Gerhard ^a  

^a UMEÅ UNIVERSITY   (Sweden)

^b UNIVERSITY OF GOTHENBURG   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIMYCIN A2; CITRININ; PHOSPHOLIPID; POLIDOCANOL; PROTEIN BAX; PROTEIN BCL 2; TRITON X 100; UNCLASSIFIED DRUG; ANTIMYCIN A1; DETERGENT; DIMYRISTOYLPHOSPHATIDYLCHOLINE; LIPID BILAYER; MACROGOL DERIVATIVE; MICELLE; OCTOXINOL; PROTEIN BAK; PROTEIN BINDING; PROTEOLIPID; PROTEOLIPOSOMES; RECOMBINANT PROTEIN;

ARTICLE; BINDING AFFINITY; BIOPHYSICS; CIRCULAR DICHROISM; COMPLEX FORMATION; CRITICAL MICELLE CONCENTRATION; DRUG PROTEIN BINDING; FLUORESCENCE SPECTROSCOPY; LIPID MEMBRANE; MEMBRANE RECONSTITUTION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN PROTEIN INTERACTION; SOLUBILITY; SURFACE PLASMON RESONANCE; ULTRACENTRIFUGATION; ULTRAVIOLET SPECTROSCOPY; CHEMISTRY; HUMAN; ISOLATION AND PURIFICATION; LIPID BILAYER; MICELLE;

ANTIMYCIN A; BCL-2 HOMOLOGOUS ANTAGONIST-KILLER PROTEIN; BCL-2-ASSOCIATED X PROTEIN; CIRCULAR DICHROISM; DETERGENTS; DIMYRISTOYLPHOSPHATIDYLCHOLINE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; LIPID BILAYERS; MICELLES; OCTOXYNOL; POLYETHYLENE GLYCOLS; PROTEIN BINDING; PROTEOLIPIDS; RECOMBINANT PROTEINS; SURFACE PLASMON RESONANCE;

EID: 84876544977     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0061452     Document Type: Article

References (37)

1. Kerr JF, Wyllie AH, Currie AR, (1972) Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 26: 239-257.
2. Twomey C, McCarthy JV, (2005) Pathways of apoptosis and importance in development. J Cell Mol Med 9: 345-359.
3. Youle RJ, Strasser A, (2008) The BCL-2 protein family: opposing activities that mediate cell death. Nat Rev Mol Cell Biol 9: 47-59.
4. Ryter SW, Kim HP, Hoetzel A, Park JW, Nakahira K, et al. (2007) Mechanisms of cell death in oxidative stress. Antioxid Redox Signal 9: 49-89.
5. Adams JM, Cory S, (2007) The Bcl-2 apoptotic switch in cancer development and therapy. Oncogene 26: 1324-1337.
6. Wolter KG, Hsu YT, Smith CL, Nechushtan A, Xi XG, et al. (1997) Movement of Bax from the cytosol to mitochondria during apoptosis. J Cell Biol 139: 1281-1292.
7. Yin XM, Oltvai ZN, Korsmeyer SJ, (1994) BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax. Nature 369: 321-323.
8. Oltvai ZN, Milliman CL, Korsmeyer SJ, (1993) Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 74: 609-619.
9. Akao Y, Otsuki Y, Kataoka S, Ito Y, Tsujimoto Y, (1994) Multiple subcellular localization of bcl-2: detection in nuclear outer membrane, endoplasmic reticulum membrane, and mitochondrial membranes. Cancer Res 54: 2468-2471.
10. Reed JC, (2008) Bcl-2-family proteins and hematologic malignancies: history and future prospects. Blood 111: 3322-3330.
11. O'Neill J, Manion M, Schwartz P, Hockenbery DM, (2004) Promises and challenges of targeting Bcl-2 anti-apoptotic proteins for cancer therapy. Biochim Biophys Acta 1705: 43-51.
12. Berghella AM, Pellegrini P, Contasta I, Del Beato T, Adorno D, (1998) Bcl-2 and drugs used in the treatment of cancer: new strategies of biotherapy which should not be underestimated. Cancer Biother Radiopharm 13: 225-237.
13. Fletcher JI, Meusburger S, Hawkins CJ, Riglar DT, Lee EF, et al. (2008) Apoptosis is triggered when prosurvival Bcl-2 proteins cannot restrain Bax. Proc Natl Acad Sci U S A 105: 18081-18087.
14. Kim H, Rafiuddin-Shah M, Tu HC, Jeffers JR, Zambetti GP, et al. (2006) Hierarchical regulation of mitochondrion-dependent apoptosis by BCL-2 subfamilies. Nat Cell Biol 8: 1348-1358.
15. Zhang Z, Lapolla SM, Annis MG, Truscott M, Roberts GJ, et al. (2004) Bcl-2 homodimerization involves two distinct binding surfaces, a topographic arrangement that provides an effective mechanism for Bcl-2 to capture activated Bax. J Biol Chem 279: 43920-43928.
16. Lin B, Kolluri SK, Lin F, Liu W, Han YH, et al. (2004) Conversion of Bcl-2 from protector to killer by interaction with nuclear orphan receptor Nur77/TR3. Cell 116: 527-540.
17. Hsu YT, Youle RJ, (1997) Nonionic detergents induce dimerization among members of the Bcl-2 family. J Biol Chem 272: 13829-13834.
18. Nedelkina S, Gokce I, Ridley H, Weckerle C, Magnin T, et al. (2008) High-yield expression and purification of soluble forms of the anti-apoptotic Bcl-x(L) and Bcl-2 as TolAIII-fusion proteins. Protein Expr Purif 60: 214-220.
19. Kolluri SK, Zhu X, Zhou X, Lin B, Chen Y, et al. (2008) A short Nur77-derived peptide converts Bcl-2 from a protector to a killer. Cancer Cell 14: 285-298.
20. Kim KM, Giedt CD, Basanez G, O'Neill JW, Hill JJ, et al. (2001) Biophysical characterization of recombinant human Bcl-2 and its interactions with an inhibitory ligand, antimycin A. Biochemistry 40: 4911-4922.
21. Tzung SP, Kim KM, Basanez G, Giedt CD, Simon J, et al. (2001) Antimycin A mimics a cell-death-inducing Bcl-2 homology domain 3. Nat Cell Biol 3: 183-191.
22. van den Berg S, Lofdahl PA, Hard T, Berglund H, (2006) Improved solubility of TEV protease by directed evolution. J Biotechnol 121: 291-298.
23. Wallgren M, Lidman M, Pham QD, Cyprych K, Grobner G, (2012) The oxidized phospholipid PazePC modulates interactions between Bax and mitochondrial membranes. Biochim Biophys Acta 1818: 2718-2724.
24. Pedersen A, Hellberg K, Enberg J, Karlsson BG, (2010) Rational improvement of cell-free protein synthesis. N Biotechnol 28: 218-224.
25. Pedersen A, Wallgren M, Karlsson BG, Grobner G, (2011) Expression and purification of full-length anti-apoptotic Bcl-2 using cell-free protein synthesis. Protein Expr Purif 77: 220-223.
26. Dlugosz PJ, Billen LP, Annis MG, Zhu W, Zhang Z, et al. (2006) Bcl-2 changes conformation to inhibit Bax oligomerization. EMBO J 25: 2287-2296.
27. Antonsson B, Montessuit S, Lauper S, Eskes R, Martinou JC, (2000) Bax oligomerization is required for channel-forming activity in liposomes and to trigger cytochrome c release from mitochondria. Biochem J 345 Pt 2: 271-278.
28. Hsu YT, Youle RJ, (1998) Bax in murine thymus is a soluble monomeric protein that displays differential detergent-induced conformations. J Biol Chem 273: 10777-10783.
29. Ivashyna O, Garcia-Saez AJ, Ries J, Christenson ET, Schwille P, et al. (2009) Detergent-activated BAX protein is a monomer. J Biol Chem 284: 23935-23946.
30. Sattler M, Liang H, Nettesheim D, Meadows RP, Harlan JE, et al. (1997) Structure of Bcl-xL-Bak peptide complex: recognition between regulators of apoptosis. Science 275: 983-986.
31. Muchmore SW, Sattler M, Liang H, Meadows RP, Harlan JE, et al. (1996) X-ray and NMR structure of human Bcl-xL, an inhibitor of programmed cell death. Nature 381: 335-341.
32. Chen L, Willis SN, Wei A, Smith BJ, Fletcher JI, et al. (2005) Differential targeting of prosurvival Bcl-2 proteins by their BH3-only ligands allows complementary apoptotic function. Mol Cell 17: 393-403.
33. Willis SN, Chen L, Dewson G, Wei A, Naik E, et al. (2005) Proapoptotic Bak is sequestered by Mcl-1 and Bcl-xL, but not Bcl-2, until displaced by BH3-only proteins. Genes Dev 19: 1294-1305.
34. Bleicken S, Zeth K, (2009) Conformational changes and protein stability of the pro-apoptotic protein Bax. J Bioenerg Biomembr 41: 29-40.
35. Yethon JA, Epand RF, Leber B, Epand RM, Andrews DW, (2003) Interaction with a membrane surface triggers a reversible conformational change in Bax normally associated with induction of apoptosis. J Biol Chem 278: 48935-48941.
36. Antharavally BS, Mallia KA, Rosenblatt MM, Salunkhe AM, Rogers JC, et al. (2011) Efficient removal of detergents from proteins and peptides in a spin column format. Anal Biochem 416: 39-44.
37. Holloway PW, (1973) A simple procedure for removal of Triton X-100 from protein samples. Anal Biochem 53: 304-308.