High-yield expression and purification of soluble forms of the anti-apoptotic Bcl-xL and Bcl-2 as TolAIII-fusion proteins

Protein Expression and Purification

Volumn 60, Issue 2, 2008, Pages 214-220

  Nedelkina, Svetlana ^a   Gokce, Isa ^b   Ridley, Helen ^b   Weckerle, Céline ^a   Magnin, Thierry ^a   Vallette, François ^c   Pattus, Franc ^a   Lakey, Jeremy H ^b   Bechinger, Burkhard ^a  

^a UNIVERSITÉ DE STRASBOURG   (France)

^b NEWCASTLE UNIVERSITY   (United Kingdom)

^c UNIVERSITÉ DE NANTES   (France)

Author keywords

Antimycin A; Apoptosis; Bacterial over expression; Membrane protein structure; Mitochondria; NMR; TolAIII fusion system

Indexed keywords

ESCHERICHIA COLI PROTEIN; HYBRID PROTEIN; PRIMER DNA; PROTEIN BCL 2; PROTEIN BCL X;

ANIMAL; ARTICLE; CIRCULAR DICHROISM; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; LIVER MITOCHONDRION; NUCLEOTIDE SEQUENCE; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; RAT; SPECTROFLUOROMETRY; ULTRAVIOLET SPECTROPHOTOMETRY; WISTAR RAT;

ANIMALS; BASE SEQUENCE; BCL-X PROTEIN; CIRCULAR DICHROISM; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI PROTEINS; MITOCHONDRIA, LIVER; PLASMIDS; PROTO-ONCOGENE PROTEINS C-BCL-2; RATS; RATS, WISTAR; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, FLUORESCENCE; SPECTROPHOTOMETRY, ULTRAVIOLET;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 46149115879     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.04.005     Document Type: Article

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