Expression and purification of full-length anti-apoptotic Bcl-2 using cell-free protein synthesis

Protein Expression and Purification

Volumn 77, Issue 2, 2011, Pages 220-223

  Pedersen, Anders ^a   Wallgren, Marcus ^b   Karlsson, B Göran ^a   Gröbner, Gerhard ^b  

^a UNIVERSITY OF GOTHENBURG   (Sweden)

^b UMEÅ UNIVERSITY   (Sweden)

Author keywords

Apoptosis; Bcl 2; Cell free protein synthesis; Full length protein; Optimized gene

Indexed keywords

CETOMACROGOL; HYBRID PROTEIN; ISOPROTEIN; PROTEIN BCL 2;

AFFINITY CHROMATOGRAPHY; APOPTOSIS; ARTICLE; B CELL LYMPHOMA; BIOSYNTHESIS; CELL FRACTIONATION; CELL FREE SYSTEM; CHEMICAL PHENOMENA; CHEMISTRY; CHRONIC LYMPHATIC LEUKEMIA; CIRCULAR DICHROISM; ESCHERICHIA COLI; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; PROTEIN FOLDING; PROTEIN SECONDARY STRUCTURE; PROTEIN SYNTHESIS; SOLUBILITY;

APOPTOSIS; CELL FRACTIONATION; CELL-FREE SYSTEM; CETOMACROGOL; CHROMATOGRAPHY, AFFINITY; CIRCULAR DICHROISM; CLONING, MOLECULAR; ESCHERICHIA COLI; HUMANS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; LEUKEMIA, LYMPHOCYTIC, CHRONIC, B-CELL; LYMPHOMA, B-CELL; PROTEIN BIOSYNTHESIS; PROTEIN FOLDING; PROTEIN ISOFORMS; PROTEIN STRUCTURE, SECONDARY; PROTO-ONCOGENE PROTEINS C-BCL-2; RECOMBINANT FUSION PROTEINS; SOLUBILITY; SUBCELLULAR FRACTIONS;

ESCHERICHIA COLI;

EID: 79952627633     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.02.003     Document Type: Article

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