Reverse micellar extraction of bromelain from pineapple (Ananas comosus L. Merryl) waste: Scale-up, reverse micelles characterization and mass transfer studies

Separation Science and Technology

Volumn 46, Issue 10, 2011, Pages 1656-1664

  Umesh Hebbar, H ^a   Hemavathi, A B ^a   Sumana, B ^a   Raghavarao, K S M S ^a  

^a CENTRAL FOOD TECHNOLOGICAL RESEARCH INSTITUTE   (India)

Author keywords

Bromelain; CTAB; Mass transfer; Reverse micelles; Scale up

Indexed keywords

ACTIVITY RECOVERY; ANANAS COMOSUS; BACK EXTRACTION; BROMELAIN; CETYLTRIMETHYLAMMONIUM BROMIDE; COMMERCIAL GRADE; CTAB; EXTRACTION EFFICIENCIES; EXTRACTION PROCESS; FORWARD-EXTRACTION; GEOMETRICAL MODELS; MASS TRANSFER STUDIES; PHASE COMPONENT; PHASE TRANSFER; PROCESS SCALE-UP; REAL SYSTEMS; REVERSE MICELLAR EXTRACTIONS; REVERSE MICELLES; SCALE-UP;

AMMONIUM COMPOUNDS; BROMINE COMPOUNDS; EXTRACTION; MASS TRANSFER; PARAFFINS; PURIFICATION; SURFACE ACTIVE AGENTS;

MICELLES;

EID: 79960685578     PISSN: 01496395     EISSN: 15205754     Source Type: Journal    
DOI: 10.1080/01496395.2011.572110     Document Type: Article

References (42)

1. Harikrishna, S.; Srinivas, N.D.; Raghavarao, K.S.M.S.; Karanth, N.G. (2001) Reverse micellar extraction for downstream processing of proteins/enzymes. Adv. Biochem. Eng. Biotechnol., 75: 119.
2. Goklen, K.E.; Hatton, T.A. (1987) Liquid-liquid extraction of low molecular weight proteins by solubilization in reversed micelles. Sep. Sci. Technol., 22: 831.
3. Ichikawa, S.; Imai, M.; Shimizu, M. (1992) Solubilizing water involved in protein extraction using reversed micelles. Biotechnol. Bioeng., 39: 20.
4. Poppenborg, L.H.; Brillis, A.A.; Stuckey, D.C. (2000) The kinetic separation of protein mixtures using reverse micelles. Sep. Sci. Technol., 35: 843.
5. Shin, Y.; Weber, M.E.; Vera, J.H. (2003) Reverse micellar extraction and precipitation of lysozyme using sodium di(2-ethylhexyl) sulfosuccinate. Biotechnol. Prog., 19: 928.
6. Chun, B.S.; Park, S.Y.; Kang, K.Y.; Wilkinson, G.T. (2005) Extraction of lysozyme using reverse micelles and pressurized carbon dioxide. Sep. Sci. Technol., 40: 2497.
7. Dovyap, Z.; Bayraktar, E.; Mehmetoglu, U. (2006) Amino acid extraction and mass transfer rate in the reverse micelle system. Enz. Microb. Technol., 38: 557.
8. Hebbar, H.U.; Raghavarao, K.S.M.S. (2007) Extraction of bovine serum albumin using nanoparticulate reverse micelles. Process Biochem., 42: 1602.
9. Jarudilokkul, S.; Stuckey, D.C. (2001) Continuous forward and back extraction of lysozyme from egg white using reverse micelles. Sep. Sci. Technol., 36: 657.
10. Manocha, B.; Gaikar, V.G. (2006) Permeabilization of Aspergillus niger by reverse micellar solutions and simultaneous purification of catalase. Sep. Sci. Technol., 41: 3279.
11. Hemavathi, A.B.; Hebbar, H.U.; Raghavarao, K.S.M.S. (2007) Reverse micellar extraction of bromelain from Ananas comosus L. Merryl. J. Chem. Tech. Biotechnol., 82: 985.
12. Hemavathi, A.B.; Hebbar, H.U.; Raghavarao, K.S.M.S. (2008) Reverse micellar extraction of b-galactosidase from barley (Hordeum vulgare). Appl. Biochem. Biotechnol., 151: 522.
13. Hebbar, H.U.; Sumana, B.; Raghavarao, K.S.M.S. (2008) Use of reverse micellar systems for the extraction and purification of bromelain from pineapple wastes. Bioresour. Technol., 99: 4896.
14. Zhou, X.; Jin, T.; Dong, L.; Zheng, S.; Xiao, J. (2009) Liquid-liquid extraction of GSH using DEHAP/isooctane reverse micelles. Sep. Sci. Technol., 44: 3632.
15. Hemavathi, A.B.; Hebbar, H.U.; Raghavarao, K.S.M.S. (2010) Mixed reverse micellar systems for extraction and purification of b-glucosidase. Sep. Purif. Technol., 71: 263.
16. Sriwatanapongse, A.; Balaban, M.; Teixeira, A. (2000) Thermal inactivation kinetics of bromelain in pineapple juice. Trans ASAE, 43: 1703.
17. Rowan, A.D.; Buttle, D.J.; Barrett, A.J. (1990) The cysteine proteinases of the pineapple plant. Biochem. J., 266: 869.
18. Bardiys, N.; Somayaji, D.; Khanna, S. (1996) Biomethanation of banana peel and pineapple waste. Bioresour. Technol., 58: 73.
19. Krei, G.; Meyer, U.; Borner, B.; Hustedt, H. (1995) Extraction of a-amylase using BDBAC-reversed micelles. Bioseparation, 5: 175.
20. Pessoa Jr., A.; Vitolo, M. (1997) Separation of inulinase from Kluyveromyces marxianus using reversed micellar extraction. Biotechnol. Techn., 11: 421.
21. Bonner, F.J.; Wolf, R.; Luisi, P.L. (1980) Micellar solubilization of biopolymers in hydrocarbon solvents. 1. A structural model of protein containing reverse micelles. J. Solid Phase Biochem., 5: 255.
22. Levashov, A.V.; Khmelnitsky, Y.L.; Klyachko, N.; Chernyak, V.Y.; Martinek, K. (1982) Enzymes entrapped into reversed micelles in organic solvents. J. Colloid Interf. Sci., 88: 444.
23. Sheu, E.; Goklen, K.E.; Hatton, T.A.; Chen, S.H. (1986) Small angle neutron scattering studies of protein-reversed micelle complexes. Biotechnol. Prog., 2: 175.
24. Regalado, C.; Asenjo, J.A.; Pyle, D.L. (1994) Protein extraction by revere micelles: Studies on the recovery of horseradish peroxidase. Biotechnol. Bioeng., 44: 674.
25. Krei, G.A.; Hustedt, H. (1992) Extraction of enzymes by reverse micelles. Chem. Eng. Sci., 47: 99.
26. Jolivalt, C.; Minier, M.; Renon, H. (1993) Extraction of cytochrome c in sodium dodecylbenzenesulfonate microemulsions. Biotechnol. Prog., 9: 456.
27. Bratko, D.; Luzar, A.; Chen, S.H. (1988) Electrostatic model for protein/reverse micelle complexation. J. Phy. Chem., 89: 545.
28. Woll, J.M.; Hatton, T.A. (1989) A simple phenomenological thermodynamic model for protein partitioning in reverse micellar systems. Bioprocess Eng., 4: 193.
29. Caselli, M.; Luisi, P.L.; Maestro, M.; Roselli, R. (1988) Thermodynamics of the uptake of proteins by reverse micelles: A first approximation model. J. Phy. Chem., 92: 3899.
30. Bru, R.; Sanchez-Ferrer, A.; Garcia-Carmona, F.A. (1989) Theoretical study on the expression of enzymatic activity in reverse micelles. Biochem. J., 259: 355.
31. Gaikar, V.G.; Kulkarni, M.S. (2001) Selective reversemicellar extraction of penicillin acylase from E. coli. J. Chem. Technol. Biotechnol., 76: 729.
32. Motlekar, N.A.; Bhagwat, S.S. (2001) Activity of horseradish peroxides in aqueous and reverse micelles and back extraction from reverse micellar phases. J. Chem. Technol. Biotechnol., 76: 643.
33. Kinugasa, T.; Kondo, A.; Mouri, E.; Ichikawa, S.; Nakagawa, S.; Nishii, Y.; Watanabe, K.; Takeuchi, H. (2003) Effects of ion species in aqueous phase on protein extraction into reverse micellar solution. Sep. Purif. Technol., 31: 251.
34. Dekker, M.; Leser, M.E. (1994) The Use of Reverse Micelles for the Separation of Proteins. In: Highly Selective Separation in Biotechnology; Street, G., ed.; Marcel Dekker Publishers: New York, 86-109.
35. Dungan, S.R.; Bausch, T.; Hatton, T.A.; Plucinski, P.; Nitsch, W. (1991) Interfacial transport processes in the reversed micellar extraction of proteins. J. Colloid Interf. Sci., 145: 33.
36. Chun, B.S.; Wilkinson, G.T. (2002) Mass-transfer of lysozyme across a flat surface with reverse micelles. Sep. Sci. Technol., 37: 3323.
37. Murachi, T. (1976) Bromelain Enzymes. In: Methods in Enzymology; Lorand, L., ed.; Academic Press: New York, 475-485.
38. Fletcher, P.; Parrott, D. (1989) Protein Partitioning between Microemulsion Phases and Conjugate Aqueous Phase. In: Structure and Reactivity in Reversed Micelles; Pileni, M.P., ed.; Elsevier: Amsterdam, 303-313.
39. Rabie, H.R.; Vera, J.H. (1996) Generalized water uptake modeling of water-in-oil microemulsions: New experimental results for AOTisooctane-water-salts systems. Fluid Phase Equilibria., 122: 169.
40. Kinugasa, T.; Kondo, A.; Nishimura, S.; Miyauchi, Y.; Nishii, Y.; Watanabe, K.; Tukeuchi, H. (2002) Estimation for size of revere micelles formed by AOT and SDEHP based on viscosity measurement. Colloid Surf. A: Physicochem. Eng. Aspects, 204: 193.
41. Chang, Q.; Liu, H.; Chen, J. (1994) Extraction of lysozyme, a-chymotrypsin, and pepsin into reverse micelles formed using an anionic surfactant, isooctane, and water. Enz. Microb. Technol., 16: 970.
42. Liu, Y.; Dong, X.Y.; Sun, Y. (2006) Equilibria and kinetics of protein transfer to and from affinity based reverse micelles of Span 85 modified with Cibracron Blue F-3GA. Biochem. Eng. J., 28: 281.