Reverse micelles extraction of nattokinase from fermentation broth

Biochemical Engineering Journal

Volumn 21, Issue 3, 2004, Pages 273-278

  Liu, Jun Guo ^a,b,c   Xing, Jian Min ^a   Shen, Rui ^a   Yang, Cheng Li ^a   Liu, Hui Zhou ^a  

^a INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^b HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^c UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

AOT; Nattokinase purification; Protein purification; Reverse micelles

Indexed keywords

FERMENTATION BROTH; FIBRIN; NATTOKINASE; VITRO;

CONCENTRATION (PROCESS); ENZYMES; EXTRACTION; FERMENTATION; PH EFFECTS; PURIFICATION; SURFACE ACTIVE AGENTS;

MICELLES;

2 PROPANOL; NATTOKINASE; OCTANE; SUBTILISIN; SURFACTANT; UNCLASSIFIED DRUG;

ARTICLE; ENZYME ACTIVITY; ENZYME PURIFICATION; EXTRACTION; FERMENTATION; PH; PRIORITY JOURNAL; REVERSE MICELLE;

EID: 4544279725     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bej.2004.07.008     Document Type: Article

References (27)

1. H. Sumi, H. Hamado, H. Tsushima, H. Mihara, and H. Murica A novel fibrinolytic enzyme (nattokinase) in the vegetable cheese Natto: a typical and popular soybean food in the Japanese diet Experientia 43 1987 1110 1111
2. H. Sumi, H. Hamada, K. Nakanishi, and H. Hiratani Enhancement of the fibrinolytic activity in plasma by oral administration of Nattokinase Acta Haemotol. 84 1990 139 143
3. M. Fujita, K. Hong, Y. Ito, S. Misawa, N. Takeuchi, K. Kariya, and S. Nishimuro Transport of nattokinase across the rat intestinal tract Biol. Pharm. Bull. 18 1995 1194 1196
4. M. Fujita, K. Hong, Y. Ito, R. Fuji, K. Kariya, and S. Nishimuro Thrombolytic effect of Nattokinase on a chemically induced thrombosis model in rat Biol. Pharm. Bull. 18 1995 1387 1391
5. T. Urano, H. Ihara, K. Umemura, Y. Suzuki, M. Oike, S. Akita, Y. Tskamoto, I. Suzuki, and A. Takada The profibrinolytic enzyme subtilisin NAT purified from Bacillus subtilis cleaves and inactivates plasminogen activator inhibitor type 1 J. Biol. Chem. 276 2001 24690 24696
6. M. Fujita, K. Nomura, K. Hong, Y. Ito, A. Asada, and S. Nishimuro Purification and characterization of a strong fibrinolytic enzyme (nattokinase) in the vegetable cheese Natto, a popular soybean fermented food in Japan Biochem. Bioph. Res. Co. 197 1993 1340 1347
7. C.T. Chang, M.H. Fan, F.C. Kuo, and H.Y. Sung Potent fibrinolytic Enzyme from a mutant of Bacillus subtilis IMR-NK1 J. Agric. Food Chem. 48 2000 3210 3216
8. K. Nakanishi, K. Nomura, K. Tajima, H. Hiratani, Fibrinolytic protein and production method thereof, [P] United States Patent, 5750650, 1998.
9. P.L. Luisi, M. Giomini, M.P. Pileni, and B.H. Robinson Reverse micelles as hosts for proteins and small molecules Biochim. Biophys. Acta 947 1988 209 246
10. M. Dekker, R. Hilhorst, and C. Lanne Isolating enzyme by reversed micelles Anal. Biochem. 178 1989 217 226
11. G.J. Lye, J.A. Asenjo, and D.L. Pyle Protein extraction using reverse micelles: kinetics of protein partitioning Chem. Eng. Sci. 49 1994 3195 3204
12. G.A. Krei, and H. Hustedt Extraction of enzymes by reversed micelles Chem. Eng. Sci. 47 1992 99 111
13. N. Takashi, Y. Yamagata, and E. Ichishima Nucleotide sequence of the subtilisin NAT gene, aprN of Bacillus subtilis (natto) Biosci. Biotech. Biochem. 56 1992 1869 1871
14. http://www.rcsb.org/pdb. entry 4lyz for lysozyme, 1e7i for serum albumin, 4cha for α-chymotrypsin, 2ptn for trypsin, 1amy for α-amylase, 1xps for ribonuclase A, 1a7v for cytochrome C.
15. P.P. Wangikar, T.P. Graycar, D.A. Estell, D.S. Clark, and J.S. Dordick Protein and solvent engineering of subtilisin BPN' in nearly anhydrous organic media J. Am. Chem. Soc. 115 1993 12231 12237
16. Z.F. Xu, R. Affleck, P. Wangikar, V. Suzawa, J.S. Dordick, and D.S. Clark Transition state stabilization of subtilisins in organic media Biotechnol. Bioeng. 43 1993 515 520
17. M.M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 72 1976 248 254
18. M.L. Ansen The estimation of pepsin, trypsin, papain, and cathepsin with hemoglobin J. Gen. Physiol. 22 1939 78 79
19. M.S. Andrade, and S.M.B. Costa Structural changes of α-chymotrypsin in reverse micelles of AOT studied by steady state and transient state fluorescence spectroscopy J. Mol. Struct. 565-566 2001 219 223
20. E.P. Melo, C.M. Carvalho, M.R. Aires-Barros, S.M. Costa, and B. Deactivation Conformational changes of cutinase in reverse micelles Biotechnol. Bioeng. 58 1998 380 387
21. J.M.S. Cabral, and M.R. Aires-Barros J.F. Kennedy J.M.S. Cabral Recovery Processes for Biological Materials 1993 John Wiley & Sons UK 247 271
22. K.E. Goklen, Liquid-liquid extraction of biopolymers: selective solubilization of proteins in reverse micelles, PhD thesis, MIT, USA, 1986.
23. A.V. Kabanov, and N.L. Klyachko Engineering of functional supramolecular complexes of proteins (enzymes) using reversed micelles as a matrix microreactors Protein Eng. 4 1991 1009 1017
24. Q. Chang, H. Liu, and J. Chen Extraction of lysozyme, α-chymotrypsin, and pepsin into reverse micelles formed using an anionic surfactant, isooctane, and water Enzyme Micro. Technol. 16 1994 970 973
25. S.R. Dungan, T. Bausch, T.A. Hatton, P. Plucinski, and W. Nitsch Interfacial transport processes in the reversed micellar extraction of proteins J. Colloid Interf. Sci. 145 1991 33 50
26. A. Carlson, and R. Nagarajan Release and recovery of porcine pepsin and bovine chymosin from reverse micelles: a new technique based on isopropyl alcohol addition Biotechnol. Prog. 8 1992 85 90
27. D.P. Hong, and R. Kuboi Evaluation of the alcohol-mediated interaction between micelles using percolation processes of reverse micellar systems Biochem. Eng. J. 4 1999 23 29