Recombinant purine nucleoside phosphorylases from thermophiles: Preparation, properties and activity towards purine and pyrimidine nucleosides

FEBS Journal

Volumn 280, Issue 6, 2013, Pages 1475-1490

  Zhou, Xinrui ^a   Szeker, Kathleen ^a   Janocha, Bernd ^a,b   Böhme, Thomas ^a,b   Albrecht, Dirk ^c   Mikhailopulo, Igor A ^d   Neubauer, Peter ^a  

^a TECHNISCHE UNIVERSITÄT BERLIN   (Germany)

^b SANOFI AVENTIS DEUTSCHLAND GMBH   (Germany)

^c UNIVERSITY OF GREIFSWALD   (Germany)

^d INSTITUTE OF BIOORGANIC CHEMISTRY   (Belarus)

Author keywords

2 deoxyfluoro adenine nucleosides; Aeropyrum pernix; Deinococcus geothermalis; Geobacillus thermoglucosidasius; thermostable PNP

Indexed keywords

1 (2 DEOXY 2 FLUORO BETA DEXTRO ARABINOFURANOSYL)URACIL; 2' DEOXYFLUORO ADENINE NUCLEOSIDE; 5' METHYTHIOADENOSINE PHOSPHORYLASE; ADENINE; CYTIDINE; DEOXYCYTIDINE; NUCLEOSIDE; PURINE; PURINE NUCLEOSIDE PHOSPHORYLASE; PYRIMIDINE; PYRIMIDINE NUCLEOSIDE PHOSPHORYLASE; RECOMBINANT ENZYME; UNCLASSIFIED DRUG; URACIL;

AEROPYRUM PERNIX; ARTICLE; CATALYST; CONTROLLED STUDY; DEINOCOCCUS; DEINOCOCCUS GEOTHERMALIS; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME SUBSTRATE; GEOBACILLUS; GEOBACILLUS THERMOGLUCOSIDASIUS; MOLECULAR WEIGHT; NONHUMAN; NUCLEIC ACID SYNTHESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; REACTION OPTIMIZATION; SPECIES COMPARISON; STRUCTURE ACTIVITY RELATION; TEMPERATURE SENSITIVITY; THERMOPHILE;

AEROPYRUM; AMINO ACID SEQUENCE; CLONING, MOLECULAR; DEINOCOCCUS; DEOXYADENOSINES; DNA, BACTERIAL; ENZYME ACTIVATION; ENZYME ASSAYS; ENZYME STABILITY; ESCHERICHIA COLI; GEOBACILLUS; GLYCOSYLATION; KINETICS; MOLECULAR SEQUENCE DATA; PHOSPHORYLATION; PURINE NUCLEOSIDES; PURINE-NUCLEOSIDE PHOSPHORYLASE; PYRIMIDINE NUCLEOSIDES; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SOLUBILITY; SUBSTRATE SPECIFICITY; TEMPERATURE;

AEROPYRUM PERNIX; ARCHAEA; DEINOCOCCUS GEOTHERMALIS; GEOBACILLUS THERMOGLUCOSIDASIUS;

EID: 84875364367     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/febs.12143     Document Type: Article

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