Purification and characterization of 5′-methylthioadenosine phosphorylase from the hyperthermophilic archaeon Pyrococcus furiosus: Substrate specificity and primary structure analysis

Extremophiles

Volumn 7, Issue 2, 2003, Pages 159-168

  Cacciapuoti, Giovanna ^a   Bertoldo, Costanzo ^b   Brio, Assunta ^a   Zappia, Vincenzo ^a   Porcelli, Marina ^a  

^a SECOND UNIVERSITY OF NAPLES   (Italy)

^b HAMBURG UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

5 Methylthioadenosine phosphorylase; Disulfide bridges; Protein hyperthemostability; Purine nucleoside phosphorylase; Pyrococcus furiosus

Indexed keywords

ARCHAEA; EUKARYOTA; PYROCOCCUS FURIOSUS; SULFOLOBUS SOLFATARICUS;

5' METHYLTHIOADENOSINE PHOSPHORYLASE; 5'-METHYLTHIOADENOSINE PHOSPHORYLASE; DISULFIDE; PURINE NUCLEOSIDE PHOSPHORYLASE; REDUCING AGENT;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; COMPARATIVE STUDY; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; GENE; GENETICS; HEAT; HUMAN; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR GENETICS; MOLECULAR WEIGHT; PYROCOCCUS FURIOSUS; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; DISULFIDES; ENZYME STABILITY; GENES, ARCHAEAL; HEAT; HUMANS; KINETICS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PURINE-NUCLEOSIDE PHOSPHORYLASE; PYROCOCCUS FURIOSUS; REDUCING AGENTS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

EID: 0642307162     PISSN: 14310651     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00792-002-0307-2     Document Type: Article

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