Mutagenesis of a specificity-determining residue in tyrosine hydroxylase establishes that the enzyme is a robust phenylalanine hydroxylase but a fragile tyrosine hydroxylase

Biochemistry

Volumn 52, Issue 8, 2013, Pages 1446-1455

  Daubner, S Colette ^a   Avila, Audrey ^a   Bailey, Johnathan O ^b   Barrera, Dimitrios ^a   Bermudez, Jaclyn Y ^a   Giles, David H ^c   Khan, Crystal A ^c   Shaheen, Noel ^a   Thompson, Janie Womac ^b   Vasquez, Jessica ^b   Oxley, Susan P ^a   Fitzpatrick, Paul F ^c  

^a ST MARY'S UNIVERSITY   (United States)

^b TEXAS A AND M UNIVERSITY   (United States)

^c Mays Cancer Center at UT Health San Antonio   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; ALANINE-SCANNING MUTAGENESIS; AMINO ACID RESIDUES; AROMATIC AMINO ACID HYDROXYLASES; FITZPATRICK; HYDROPHOBIC INTERACTIONS; MUTANT PROTEINS; NEGATIVE CORRELATION; PHENYLALANINE HYDROXYLASE; SATURATION MUTAGENESIS; SUBSTRATE SPECIFICITY; TYROSINE HYDROXYLASE;

ENZYME ACTIVITY; HYDROPHOBICITY; HYDROXYLATION; MUTAGENESIS; SUBSTRATES;

AMINO ACIDS;

ALANINE; AMINO ACID; TYROSINE 3 MONOOXYGENASE;

ARTICLE; ENZYME ACTIVITY; HYDROPHOBICITY; HYDROXYLATION; MUTAGENESIS; MUTANT; NONHUMAN; PRIORITY JOURNAL;

ALANINE; AMINO ACID SUBSTITUTION; AMINO ACIDS; ANIMALS; HYDROXYLATION; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PHENYLALANINE HYDROXYLASE; PTERINS; RATS; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; TYROSINE 3-MONOOXYGENASE;

EID: 84874416569     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi400031n     Document Type: Article

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