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Volumn 122, Issue 19, 2000, Pages 4535-4541

On the catalytic mechanism of tryptophan hydroxylase

Author keywords

[No Author keywords available]

Indexed keywords

DEUTERIUM; OXYGEN; TETRAHYDROBIOPTERIN; TRYPTOPHAN; TRYPTOPHAN HYDROXYLASE; TYROSINE;

EID: 0034679014     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja994479a     Document Type: Article
Times cited : (51)

References (46)
  • 34
    • 0002378469 scopus 로고
    • Origins and interpretations of heavy-atom isotope effects
    • Cook, P. F., Ed.; CRC Press: Boca Raton
    • Huskey, W. P. Origins and interpretations of heavy-atom isotope effects.; In Enzyme mechanism from isotope effects; Cook, P. F., Ed.; CRC Press: Boca Raton, 1991: pp 37-72.
    • (1991) Enzyme Mechanism from Isotope Effects , pp. 37-72
    • Huskey, W.P.1
  • 43
    • 0343672528 scopus 로고    scopus 로고
    • note
    • Because of the assumptions made in calculating the isotope effects, we have made no attempt to correlate the magnitude of the isotope effect with the structure of the transition state. The critical feature of the calculated effects is that formation of I should result in an inverse isotope effect only upon deuteration of carbon 5, while formation of II will result in inverse effects upon deuteration at either position. Thus, it is the relative magnitudes of the effects at the two positions which is diagnostic of mechanism. The values measured with 295 μM oxygen are clearly not the intrinsic effects, as demonstrated by the more inverse effect seen at 830 μM oxygen.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.