Aggregation of anti-streptavidin immunoglobulin gamma-1 involves Fab unfolding and competing growth pathways mediated by pH and salt concentration

Biophysical Chemistry

Volumn 172, Issue , 2013, Pages 26-36

  Kim, Nayoung ^a   Remmele, Richard L ^b,e   Liu, Dingjiang ^b,f   Razinkov, Vladimir I ^c   Fernandez, Erik J ^d   Roberts, Christopher J ^a  

^a UNIVERSITY OF DELAWARE   (United States)

^b AMGEN INC   (United States)

^c AMGEN INC   (United States)

^d University of Virginia   (United States)

^e MEDIMMUNE   (United States)

^f REGENERON PHARMACEUTICALS INC   (United States)

Author keywords

Aggregation; Antibody; Protein interaction; Unfolding

Indexed keywords

IMMUNOGLOBULIN ANTIBODY; SODIUM CHLORIDE; STREPTAVIDIN IMMUNOGLOBULIN GAMMA 1 ANTIBODY; THIOFLAVINE; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; CALORIMETRY; CHROMATOGRAPHY; CIRCULAR DICHROISM; CONFORMATIONAL TRANSITION; DIFFERENTIAL SCANNING CALORIMETRY; FLUORESCENCE SPECTROSCOPY; HIGH TEMPERATURE; INFRARED SPECTROSCOPY; LIGHT SCATTERING; PH; PHASE SEPARATION; PRIORITY JOURNAL; PROTEIN UNFOLDING; STATIC ELECTRICITY; THERMODYNAMICS;

EID: 84873886259     PISSN: 03014622     EISSN: 18734200     Source Type: Journal    
DOI: 10.1016/j.bpc.2012.12.004     Document Type: Article

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