Assessment of immobilized PGA orientation via the LC-MS analysis of tryptic digests of the wild type and its 3K-PGA mutant assists in the rational design of a high-performance biocatalyst

Analytical and Bioanalytical Chemistry

Volumn 405, Issue 2-3, 2013, Pages 745-753

  Serra, Immacolata ^a   Ubiali, Daniela ^a   Cecchini, Davide A ^a,b   Calleri, Enrica ^a   Albertini, Alessandra M ^a   Terreni, Marco ^a   Temporini, Caterina ^a  

^a UNIVERSITY OF PAVIA   (Italy)

^b INSA   (France)

Author keywords

Immobilized protein orientation; Liquid chromatography tandem mass spectrometry; Penicillin G acylase; Site directed mutagenesis

Indexed keywords

ALDEHYDES; DRUG PRODUCTS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MUTAGENESIS; PEPTIDES;

ACCESSIBLE SURFACE AREAS; ANALYTICAL CHARACTERIZATION; HIGH-PERFORMANCE BIOCATALYST; IMMOBILIZED PROTEINS; LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY (LC MS/MS); LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY; PENICILLIN G ACYLASE; SITE DIRECTED MUTAGENESIS;

ENZYMES;

EID: 84873732314     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-012-6143-z     Document Type: Article

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