The role of hydrophobic active-site residues in substrate specificity and acyl transfer activity of penicillin acylase

European Journal of Biochemistry

Volumn 269, Issue 8, 2002, Pages 2093-2100

  Alkema, Wynand B L ^a   Dijkhuis, Anne Jan ^a   De Vries, Erik ^a   Janssen, Dick B ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

lactam antibiotics; Penicillin acylase; Site directed mutagenesis; Substrate specificity; Transferase hydrolase ratio

Indexed keywords

2 NITRO 5 [(PHENYLACETYL)AMINO]BENZOIC ACID; 6 AMINOPENICILLANIC ACID; ALANINE; AMIDASE; AMOXICILLIN; AMPICILLIN; BENZOIC ACID DERIVATIVE; CEFADROXIL; CEFALEXIN; CEPHALOSPORIN DERIVATIVE; ESTER DERIVATIVE; HYDROLASE; LEUCINE; OXFENICINE; PENICILLIN DERIVATIVE; PHENYLGLYCINE; TRANSFERASE; TRYPTOPHAN; TYROSINE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DRUG SYNTHESIS; ENZYME ACTIVITY; ENZYME SPECIFICITY; HYDROLYSIS; MUTATION; NONHUMAN; PRIORITY JOURNAL;

AMINOBENZOIC ACIDS; ANTI-BACTERIAL AGENTS; BINDING SITES; ESCHERICHIA COLI; KINETICS; LACTAMS; MUTAGENESIS, SITE-DIRECTED; NITROBENZOATES; PENICILLIN AMIDASE; PHENYLACETATES; PHENYLALANINE; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; TRIXIS;

EID: 0036237518     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2002.02857.x     Document Type: Article

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