A HaloTag® method for assessing the retrograde axonal transport of the p75 neurotrophin receptor and other proteins in compartmented cultures of rat sympathetic neurons

Journal of Neuroscience Methods

Volumn 214, Issue 1, 2013, Pages 91-104

  Mok, Sue Ann ^a,b   Lund, Karen ^a   LaPointe, Paul ^a   Campenot, Robert B ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Anterograde transport; Compartmented culture; HaloTag ; Neurotrophin; Retrograde signaling; Retrograde transport; Sympathetic neurons

Indexed keywords

ALPHA TUBULIN; BRAIN DERIVED NEUROTROPHIC FACTOR; CELL PROTEIN; HALOTAG PROTEIN; HYBRID PROTEIN; MEMBRANE PROTEIN; NERVE GROWTH FACTOR; NEUROTROPHIN RECEPTOR P75; NEUROTROPIN; TETRAMETHYLRHODAMINE; TUBULIN ALPHA1B; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANIMAL EXPERIMENT; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL COMPARTMENTALIZATION; CERVICAL GANGLION; CONTROLLED STUDY; CYTOSOL; FLUORESCENCE; GENETIC TRANSFECTION; IMMUNOBLOTTING; NERVE CELL CULTURE; NERVE FIBER; NEWBORN; NONHUMAN; PERIKARYON; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN TRANSPORT; RAT; RETROGRADE NERVE FIBER TRANSPORT; SIGNAL TRANSDUCTION; SYMPATHETIC NERVE CELL;

AMINO ACID SEQUENCE; ANIMALS; AXONAL TRANSPORT; BASE SEQUENCE; CELL CULTURE TECHNIQUES; CELLS, CULTURED; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ELECTROPORATION; FLUORESCENT DYES; FLUOROMETRY; HYDROLASES; IMMUNOBLOTTING; LIGANDS; MOLECULAR SEQUENCE DATA; NERVE TISSUE PROTEINS; NEURONS; RATS; RATS, SPRAGUE-DAWLEY; RECEPTORS, NERVE GROWTH FACTOR; RECOMBINANT FUSION PROTEINS; RHODAMINES; SUPERIOR CERVICAL GANGLION; TRANSFECTION; TUBULIN;

EID: 84873657816     PISSN: 01650270     EISSN: 1872678X     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2013.01.006     Document Type: Article

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