Examining the complexity of human RNA polymerase complexes using HaloTag technology coupled to label free quantitative proteomics

Journal of Proteome Research

Volumn 11, Issue 2, 2012, Pages 564-575

  Daniels, Danette L ^a   Méndez, Jacqui ^a   Mosley, Amber L ^b   Ramisetty, Sreenivasa R ^c   Murphy, Nancy ^a   Benink, Hélène ^a   Wood, Keith V ^a   Urh, Marjeta ^a   Washburn, Michael P ^c,d  

^a PROMEGA CORPORATION   (United States)

^b INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c STOWERS INSTITUTE FOR MEDICAL RESEARCH   (United States)

^d UNIVERSITY OF KANSAS SCHOOL OF MEDICINE   (United States)

Author keywords

cellular imaging; mass spectrometry; MudPIT; normalized spectral abundance factor; protein interactions; quantitative proteomics; RNA polymerases

Indexed keywords

HALOTAG PROTEIN; PROTEIN DERIVATIVE; RNA POLYMERASE; RNA POLYMERASE II; UNCLASSIFIED DRUG;

ARTICLE; CELLULAR DISTRIBUTION; EMBRYO; EUKARYOTE; HUMAN; HUMAN CELL; IMAGE ANALYSIS; IN VIVO STUDY; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN INTERACTION; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEOMICS; QUANTITATIVE STUDY;

CELL LINE; CELL NUCLEUS; COMPUTATIONAL BIOLOGY; CYTOPLASM; DATABASES, PROTEIN; DNA-DIRECTED RNA POLYMERASES; HEK293 CELLS; HUMANS; MASS SPECTROMETRY; MICROSCOPY, FLUORESCENCE; MOLECULAR PROBES; MULTIPROTEIN COMPLEXES; PROTEIN SUBUNITS; PROTEOMICS;

EUKARYOTA;

EID: 84856654953     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr200459c     Document Type: Article

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