Production of compartmented cultures of rat sympathetic neurons

Nature Protocols

Volumn 4, Issue 12, 2009, Pages 1869-1887

  Campenot, Robert B ^a   Lund, Karen ^a   Mok, Sue Ann ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ASCORBIC ACID; METHYLCELLULOSE; NERVE GROWTH FACTOR;

ARTICLE; CERVICAL GANGLION; CULTURE MEDIUM; LAMINAR FLOW; LIPOGENESIS; NERVE CELL CULTURE; NERVE ENDING; NERVE FIBER GROWTH; NERVE FIBER REGENERATION; NERVE FIBER TRANSECTION; NERVE FIBER TRANSPORT; NEUROTRANSMISSION; NONHUMAN; PRIORITY JOURNAL; SYMPATHETIC NERVE;

ANIMALS; AXONS; CELL CULTURE TECHNIQUES; NEURONS; RATS; SILICONE OILS; SYMPATHETIC NERVOUS SYSTEM;

RATTUS;

EID: 74949091140     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2009.210     Document Type: Article

References (70)

1. Campenot, R.B. Local control of neurite development by nerve growth factor. Proc. Natl. Acad. Sci. USA 74, 4516-4519 (1977).
2. Campenot, R.B. Development of sympathetic neurons in compartmentalized cultures. II. Local control of neurite survival by nerve growth factor. Dev. Biol. 93, 13-21 (1982).
3. Campenot, R.B. Development of sympathetic neurons in compartmentalized cultures. I. Local control of neurite growth by nerve growth factor. Dev. Biol. 93, 1-12 (1982).
4. MacInnis, B.L. & Campenot, R.B. Retrograde support of neuronal survival without retrograde transport of nerve growth factor. Science 295, 1536-1539 (2002).
5. Mok, S.A., Lund, K. & Campenot, R.B. A retrograde apoptotic signal originating in NGF-deprived distal axons of rat sympathetic neurons in compartmented cultures. Cell Res. 19, 546-560 (2009).
6. Senger, D.L. & Campenot, R.B. Rapid retrograde tyrosine phosphorylation of trkA and other proteins in rat sympathetic neurons in compartmented cultures. J. Cell. Biol. 138, 411-421 (1997).
7. MacInnis, B.L., Senger, D.L. & Campenot, R.B. Spatial requirements for TrkA kinase activity in the support of neuronal survival and axon growth in rat sympathetic neurons. Neuropharmacology 45, 995-1010 (2003).
8. Mok, S.A. & Campenot, R.B. A nerve growth factor-induced retrograde survival signal mediated by mechanisms downstream of TrkA. Neuropharmacology 52, 270-278 (2007).
9. Eng, H., Lund, K. & Campenot, R.B. Synthesis of beta-tubulin, actin, and other proteins in axons of sympathetic neurons in compartmented cultures. J. Neurosci. 19, 1-9 (1999).
10. Vance, J.E., Pan, D., Campenot, R.B., Bussiere, M. & Vance, D.E. Evidence that the major membrane lipids, except cholesterol, are made in axons of cultured rat sympathetic neurons. J. Neurochem. 62, 329-337 (1994).
11. Campenot, R.B., Lund, K. & Senger, D.L. Delivery of newly synthesized tubulin to rapidly growing distal axons of sympathetic neurons in compartmented cultures. J. Cell. Biol. 135, 701-709 (1996).
12. Ure, D.R. & Campenot, R.B. Retrograde transport and steady-state distribution of 125I-nerve growth factor in rat sympathetic neurons in compartmented cultures. J. Neurosci. 17, 1282-1290 (1997).
13. Walicke, P.A., Campenot, R.B. & Patterson, P.H. Determination of transmitter function by neuronal activity. Proc. Natl. Acad. Sci. USA 74, 5767-5771 (1977).
14. Singh, K.K. & Miller, F.D. Activity regulates positive and negative neurotrophin-derived signals to determine axon competition. Neuron. 45, 837-845 (2005).
15. Kimpinski, K., Campenot, R.B. & Mearow, K. Effects of the neurotrophins nerve growth factor, neurotrophin-3, and brain-derived neurotrophic factor (BDNF) on neurite growth from adult sensory neurons in compartmented cultures. J. Neurobiol. 33, 395-410 (1997).
16. Watson, F.L. et al. Rapid nuclear responses to target-derived neurotrophins require retrograde transport of ligand-receptor complex. J. Neurosci. 19, 7889-7900 (1999).
17. Hayashi, H., Campenot, R.B., Vance, D.E. & Vance, J.E. Glial lipoproteins stimulate axon growth of central nervous system neurons in compartmented cultures. J. Biol. Chem. 279, 14009-14015 (2004).
18. Watson, F.L. et al. Neurotrophins use the Erk5 pathway to mediate a retrograde survival response. Nat. Neurosci. 4, 4 (2001).
19. Ye, H., Kuruvilla, R., Zweifel, L.S. & Ginty, D.D. Evidence in support of signaling endosome-based retrograde survival of sympathetic neurons. Neuron. 39, 57-68 (2003).
20. Campenot, R.B. NGF uptake and retrograde signaling mechanisms in sympathetic neurons in compartmented cultures. in Results and Problems in Cell Differentiation, Vol.48 Cell Biology of the Axon (ed. Koenig, E.) 141-158 (Springer, Berlin/Heidelberg, 2009).
21. Zweifel, L.S., Kuruvilla, R. & Ginty, D.D. Functions and mechanisms of retrograde neurotrophin signalling. Nat. Rev. Neurosci. 6, 615-625 (2005).
22. Ginty, D.D. & Segal, R.A. Retrograde neurotrophin signaling: Trk-ing along the axon. Curr. Opin. Neurobiol. 12, 268-274 (2002).
23. Campenot, R.B. & MacInnis, B.L. Retrograde transport of neurotrophins: fact and function. J. Neurobiol. 58, 217-229 (2004).
24. Campenot, R.B. & Eng, H. Protein synthesis in axons and its possible functions. J. Neurocytol. 29, 793-798 (2000).
25. Vance, J.E., Campenot, R.B. & Vance, D.E. The synthesis and transport of lipids for axonal growth and nerve regeneration. Biochim. Biophys. Acta 1486, 84-96 (2000).
26. Bertrand, J., Winton, M.J., Rodriguez-Hernandez, N., Campenot, R.B. & McKerracher, L. Application of rho antagonist to neuronal cell bodies promotes neurite growth in compartmented cultures and regeneration of retinal ganglion cell axons in the optic nerve of adult rats. J. Neurosci. 25, 1113-1121 (2005).
27. Yang, X.M. et al. Autocrine hepatocyte growth factor provides a local mechanism for promoting axonal growth. J. Neurosci. 18, 8369-8381 (1998).
28. Chu, G.K. & Tator, C.H. Calcium influx is necessary for optimal regrowth of transected neurites of rat sympathetic ganglion neurons in vitro. Neuroscience 102, 945-957 (2001).
29. Song, M.S., Saavedra, L. & de Chaves, E.I. Apoptosis is secondary to non-apoptotic axonal degeneration in neurons exposed to Abeta in distal axons. Neurobiol. Aging 27, 1224-1238 (2006).
30. Chng, T.H. & Enquist, L.W. Neuron-to-cell spread of pseudorabies virus in a compartmented neuronal culture system. J. Virol. 79, 10875-10889 (2005).
31. Chng, T.H., Spear, P.G., Struyf, F. & Enquist, L.W. Glycoprotein D-independent spread of pseudorabies virus infection in cultured peripheral nervous system neurons in a compartmented system. J. Virol. 81, 10742-10757 (2007).
32. Manning, P.T., Johnson, E.M., Jr., Wilcox, C.L., Palmatier, M.A. & Russell, J.H. MHC-specific cytotoxic T lymphocyte killing of dissociated sympathetic neuronal cultures. Am. J. Pathol. 128, 395-409 (1987).
33. Pazyra-Murphy, M.F. & Segal, R.A. Preparation and maintenance of dorsal root ganglia neurons in compartmented cultures. J. Vis. Exp. published online, doi: 10.3791/951 (17 October 2008).
34. Cui, B. et al. One at a time, live tracking of NGF axonal transport using quantum dots. Proc. Natl. Acad. Sci. USA 104, 13666-13671 (2007).
35. Heerssen, H.M. & Segal, R.A. Location, location, location: a spatial view of neurotrophin signal transduction. Trends Neurosci. 25, 160-165 (2002).
36. Heerssen, H.M., Pazyra, M.F. & Segal, R.A. Dynein motors transport activated Trks to promote survival of target-dependent neurons. Nat. Neurosci. (2004).
37. Carlton, E. et al. Fusion of the tetanus toxin C fragment binding domain and Bcl-xL for protection of peripheral nerve neurons. Neurosurgery 63, 1175-1182; discussion 1182-1184 (2008).
38. Ng, B.K., Chen, L., Mandemakers, W., Cosgaya, J.M. & Chan, J.R. Anterograde transport and secretion of brain-derived neurotrophic factor along sensory axons promote Schwann cell myelination. J. Neurosci. 27, 7597-7603 (2007).
39. Bi, J., Tsai, N.P., Lin, Y.P., Loh, H.H. & Wei, L.N. Axonal mRNA transport and localized translational regulation of kappa-opioid receptor in primary neurons of dorsal root ganglia. Proc. Natl. Acad. Sci. USA 103, 19919-19924 (2006).
40. Bi, J., Tsai, N.P., Lu, H.Y., Loh, H.H. & Wei, L.N. Copb1-facilitated axonal transport and translation of kappa opioid-receptor mRNA. Proc. Natl. Acad. Sci. USA 104, 13810-13815 (2007).
41. Copray, S., Liem, R., Mantingh-Otter, I.J. & Brouwer, N. Coculture of rat embryonic proprioceptive sensory neurons and myotubes. Muscle Nerve 19, 1401-1412 (1996).
42. Silva, A., Wang, Q., Wang, M., Ravula, S.K. & Glass, J.D. Evidence for direct axonal toxicity in vincristine neuropathy. J. Peripher. Nerv. Syst. 11, 211-216 (2006).
43. Melli, G., Keswani, S.C., Fischer, A., Chen, W. & Hoke, A. Spatially distinct and functionally independent mechanisms of axonal degeneration in a model of HIV-associated sensory neuropathy. Brain 129, 1330-1338 (2006).
44. Guertin, A.D., Zhang, D.P., Mak, K.S., Alberta, J.A. & Kim, H.A. Microanatomy of axon/glial signaling during Wallerian degeneration. J. Neurosci. 25, 3478-3487 (2005).
45. Edstrom, A. & Ekstrom, P.A. Role of phosphatidylinositol 3-kinase in neuronal survival and axonal outgrowth of adult mouse dorsal root ganglia explants. J. Neurosci. Res. 74, 726-735 (2003).
46. Kimpinski, K., Jelinski, S. & Mearow, K. The anti-p75 antibody, MC192, and brain-derived neurotrophic factor inhibit nerve growth factor-dependent neurite growth from adult sensory neurons. Neuroscience 93, 253-263 (1999).
47. Tsiang, H., Ceccaldi, P.E. & Lycke, E. Rabies virus infection and transport in human sensory dorsal root ganglia neurons. J. Gen. Virol. 72 (Part 5): 1191-1194 (1991).
48. Lycke, E. & Tsiang, H. Rabies virus infection of cultured rat sensory neurons. J. Virol. 61, 2733-2741 (1987).
49. Ekstrom, P.A., Bergstrand, H. & Edstrom, A. Effects of protein kinase inhibitors on regeneration in vitro of adult frog sciatic sensory axons. J. Neurosci. Res. 31, 462-469 (1992).
50. Ekstrom, P.A. Insulin stimulates ganglionic protein synthesis and reduces thymidine incorporation in support cells of the in vitro regenerating adult frog sciatic sensory neurons. Neurosci. Lett. 132, 183-186 (1991).
51. Ivins, K.J., Bui, E.T. & Cotman, C.W. Beta-amyloid induces local neurite degeneration in cultured hippocampal neurons: evidence for neuritic apoptosis. Neurobiol. Dis. 5, 365-378 (1998).
52. Underhill, S.M. & Goldberg, M.P. Hypoxic injury of isolated axons is independent of ionotropic glutamate receptors. Neurobiol. Dis. 25, 284-290 (2007).
53. Hayashi, H. et al. Methods for the study of lipid metabolism in neurons. Anal. Biochem. 331, 1-16 (2004).
54. Hayashi, H., Campenot, R.B., Vance, D.E. & Vance, J.E. Apolipoprotein E-containing lipoproteins protect neurons from apoptosis via a signaling pathway involving low-density lipoprotein receptor-related protein-1. J. Neurosci. 27, 1933-1941 (2007).
55. Sonderegger, P. et al. A few axonal proteins distinguish ventral spinal cord neurons from dorsal root ganglion neurons. J. Cell. Biol. 98, 364-368 (1984).
56. Klostermann, S. & Bonhoeffer, F. Investigations of signaling pathways in axon growth and guidance. Perspect. Dev. Neurobiol. 4, 237-252 (1996).
57. Brayfield, C.A., Marra, K.G., Leonard, J.P., Tracy Cui, X. & Gerlach, J.C. Excimer laser channel creation in polyethersulfone hollow fibers for compartmentalized in vitro neuronal cell culture scaffolds. Acta Biomater. 4, 244-255 (2008).
58. Bergen, J.M. & Pun, S.H. Analysis of the intracellular barriers encountered by nonviral gene carriers in a model of spatially controlled delivery to neurons. J. Gene Med. 10, 187-197 (2008).
59. Ravula, S.K., Wang, M.S., Asress, S.A., Glass, J.D. & Bruno Frazier, A. A compartmented neuronal culture system in microdevice format. J. Neurosci. Methods 159, 78-85 (2007).
60. Ravula, S.K., McClain, M.A., Wang, M.S., Glass, J.D. & Frazier, A.B. A multielectrode microcompartment culture platform for studying signal transduction in the nervous system. Lab. Chip 6, 1530-1536 (2006).
61. Liu, W.W., Goodhouse, J., Jeon, N.L. & Enquist, L.W. A microfluidic chamber for analysis of neuron-to-cell spread and axonal transport of an alpha-herpesvirus. PLoS ONE 3, e2382 (2008).
62. Taylor, A.M., Rhee, S.W. & Jeon, N.L. Microfluidic chambers for cell migration and neuroscience research. Methods. Mol. Biol. 321, 167-177 (2006).
63. Park, J.W., Vahidi, B., Taylor, A.M., Rhee, S.W. & Jeon, N.L. Microfluidic culture platform for neuroscience research. Nat. Protoc. 1, 2128-2136 (2006).
64. Campenot, R.B. Independent control of the local environment of somas and neurites. Methods Enzymol. 58, 302-307 (1979).
65. Campenot, R.B. Compartmented culture analysis of nerve growth. In Cell-Cell Interactions: A Practical Approach (eds. Stevenson, B.R., Gallin, W.J. & Paul, D.L.) 275-298 (IRL Press, Oxford, 1992).
66. Campenot, R.B. & Martin, G. Construction and use of compartmented cultures for studies of cell biology of neurons. In Protocols for Neural Cell Culture (eds. Federoff, S. & Richardson, A.) 49-57 (Humana Press, Totowa, New Jersey, 2001).
67. Hawrot, E. & Patterson, P.H. Long-term culture of dissociated sympathetic neurons. Methods Enzymol. 58, 574-584 (1979).
68. Mains, R.E. & Patterson, P.H. Primary cultures of dissociated sympathetic neurons. I. Establishment of long-term growth in culture and studies of differentiated properties. J. Cell. Biol. 59, 329-345 (1973).
69. Tsui-Pierchala, B.A. & Ginty, D.D. Characterization of an NGF-P-TrkA retrograde-signaling complex and age-dependent regulation of TrkA phosphorylation in sympathetic neurons. J. Neurosci. 19, 8207-8218 (1999).
70. Chun, L.L. & Patterson, P.H. Role of nerve growth factor in the development of rat sympathetic neurons in vitro. II. Developmental studies. J. Cell. Biol. 75, 705-711 (1977).