Insect cell-based expression and characterization of a single-chain variable antibody fragment directed against blood coagulation factor VIII

Protein Expression and Purification

Volumn 88, Issue 2, 2013, Pages 201-206

  Kurasawa, James H ^a   Shestopal, Svetlana A ^a   Jha, Naveen K ^a   Ovanesov, Mikhail V ^a   Lee, Timothy K ^a   Sarafanov, Andrey G ^a,b  

^a CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

^b CENTER FOR BIOLOGICS EVALUATION AND RESEARCH   (United States)

Author keywords

Baculovirus system; Expression; Factor VIII; Hemophilia A; Single chain variable fragment

Indexed keywords

BLOOD CLOTTING FACTOR 10A; BLOOD CLOTTING FACTOR 8; RECOMBINANT PROTEIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; THROMBIN;

ANIMAL; ARTICLE; BACULOVIRUS; BLOOD CLOTTING; CELL LINE; CYTOLOGY; DRUG ANTAGONISM; GENE EXPRESSION; GENETICS; IMMUNOLOGY; INSECT; ISOLATION AND PURIFICATION; METABOLISM; PLASMID;

ANIMALS; BACULOVIRIDAE; BLOOD COAGULATION; CELL LINE; FACTOR VIII; FACTOR XA; GENE EXPRESSION; INSECTS; PLASMIDS; RECOMBINANT PROTEINS; SINGLE-CHAIN ANTIBODIES; THROMBIN;

EID: 84873624702     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.12.008     Document Type: Article

References (42)

1. N.E. Weisser, and J.C. Hall Applications of single-chain variable fragment antibodies in therapeutics and diagnostics Biotechnol. Adv. 27 2009 502 520
2. A.A. Gilmartin, B. Lamp, T. Rumenapf, M.A. Persson, F.A. Rey, and T. Krey High-level secretion of recombinant monomeric murine and human single-chain Fv antibodies from Drosophila S2 cells Protein Eng. Des. Sel. 25 2012 59 66
3. R. Verma, E. Boleti, and A.J. George Antibody engineering: comparison of bacterial, yeast, insect and mammalian expression systems J. Immunol. Methods 216 1998 165 181 (Pubitemid 28440386)
4. E.N. van den Brink, E.A. Turenhout, N. Bovenschen, B.G. Heijnen, K. Mertens, M. Peters, and J. Voorberg Multiple VH genes are used to assemble human antibodies directed toward the A3-C1 domains of factor VIII Blood 97 2001 966 972 (Pubitemid 32154281)
5. P.J. Fay Factor VIII structure and function Int. J. Hematol. 83 2006 103 108 (Pubitemid 44227955)
6. A.H. Zhang, J. Skupsky, and D.W. Scott Factor VIII inhibitors: risk factors and methods for prevention and immune modulation Clin. Rev. Allergy Immunol. 37 2009 114 124
7. K. Mertens, A. Bovenschen, J. Voorberg, M. Rieger, and F. Scheiflinger Antagonists of Factor VIII Interaction with Low-Density Lipoprotein Receptor-Related protein US Patent 2008 20080219983A1
8. V. Limburg, C. van der Zwaan, R.C. Boertjes, N. Bovenschen, K. Mertens, and A. Meijer An antibody fragment against factor VIII that inhibits factor VIII assembly with von willebrand factor and LDL receptor family members J. Thromb. Haemostasis 3 2005 OR162
9. A. Meijer, V. Limburg, C. van der Zwaan, and K. Mertens The factor VIII C1 domain contributes to efficient LRP/LDL receptor binding J. Thromb. Haemostasis 5 2007 P-M-040
10. H. Meems, A.B. Meijer, D.B. Cullinan, K. Mertens, and G.E. Gilbert Factor VIII C1 domain residues Lys 2092 and Phe 2093 contribute to membrane binding and cofactor activity Blood 114 2009 3938 3946
11. E. Herczenik, S.D. van Haren, A. Wroblewska, P. Kaijen, M. van den Biggelaar, A.B. Meijer, L. Martinez-Pomares, B.A. Ten, and J. Voorberg Uptake of blood coagulation factor VIII by dendritic cells is mediated via its C1 domain J. Allergy Clin. Immunol. 2011
12. H. Meems, M. van den Biggelaar, M. Rondaij, C. van der Zwaan, K. Mertens, and A.B. Meijer C1 domain residues Lys 2092 and Phe 2093 are of major importance for the endocytic uptake of coagulation factor VIII Int. J. Biochem. Cell Biol. 43 2011 1114 1121
13. K. Terpe Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems Appl. Microbiol. Biotechnol. 72 2006 211 222 (Pubitemid 44273045)
14. M. Arbabi-Ghahroudi, J. Tanha, and R. MacKenzie Prokaryotic expression of antibodies Cancer Metastasis Rev. 24 2005 501 519 (Pubitemid 43096998)
15. K.J. Mukherjee, D.C. Rowe, N.A. Watkins, and D.K. Summers Studies of single-chain antibody expression in quiescent Escherichia coli Appl. Environ. Microbiol. 70 2004 3005 3012 (Pubitemid 38657819)
16. P. Oelschlaeger, S. Lange, J. Schmitt, M. Siemann, M. Reuss, and R.D. Schmid Identification of factors impeding the production of a single-chain antibody fragment in Escherichia coli by comparing in vivo and in vitro expression Appl. Microbiol. Biotechnol. 61 2003 123 132 (Pubitemid 36470193)
17. T. Ye, Z. Lin, and H. Lei High-level expression and characterization of an anti-VEGF165 single-chain variable fragment (scFv) by small ubiquitin-related modifier fusion in Escherichia coli Appl. Microbiol. Biotechnol. 81 2008 311 317
18. M. Berkmen Production of disulfide-bonded proteins in Escherichia coli Protein Expr. Purif. 82 2012 240 251
19. C. Chen, B. Snedecor, J.C. Nishihara, J.C. Joly, N. McFarland, D.C. Andersen, J.E. Battersby, and K.M. Champion High-level accumulation of a recombinant antibody fragment in the periplasm of Escherichia coli requires a triple-mutant (degP prc spr) host strain Biotechnol. Bioeng. 85 2004 463 474 (Pubitemid 38268662)
20. J. Lobstein, C.A. Emrich, C. Jeans, M. Faulkner, P. Riggs, and M. Berkmen Shuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm Microb. Cell Fact. 11 2012 56
21. G. Georgiou, and L. Segatori Preparative expression of secreted proteins in bacteria: status report and future prospects Curr. Opin. Biotechnol. 16 2005 538 545 (Pubitemid 41393835)
22. R. Schier, J.D. Marks, E.J. Wolf, G. Apell, C. Wong, J.E. McCartney, M.A. Bookman, J.S. Huston, L.L. Houston, and L.M. Weiner In vitro and in vivo characterization of a human anti-c-erbB-2 single-chain Fv isolated from a filamentous phage antibody library Immunotechnology 1 1995 73 81
23. C. Lemeulle, T. Chardes, C. Montavon, H. Chaabihi, J.C. Mani, M. Pugniere, M. Cerutti, G. Devauchelle, B. Pau, and M. Biard-Piechaczyk Anti-digoxin scFv fragments expressed in bacteria and in insect cells have different antigen binding properties FEBS Lett. 423 1998 159 166 (Pubitemid 28343201)
24. S. Padiolleau-Lefevre, C. Alexandrenne, F. Dkhissi, G. Clement, S. Essono, C. Blache, J.Y. Couraud, A. Wijkhuisen, and D. Boquet Expression and detection strategies for an scFv fragment retaining the same high affinity than Fab and whole antibody: Implications for therapeutic use in prion diseases Mol. Immunol. 44 2007 1888 1896
25. D.L. Mahiouz, G. Aichinger, D.O. Haskard, and A.J. George Expression of recombinant anti-E-selectin single-chain Fv antibody fragments in stably transfected insect cell lines J. Immunol. Methods 212 1998 149 160 (Pubitemid 28288353)
26. B. Reavy, A. Ziegler, J. Diplexcito, S.M. Macintosh, L. Torrance, and M. Mayo Expression of functional recombinant antibody molecules in insect cell expression systems Protein Expr. Purif. 18 2000 221 228 (Pubitemid 30172228)
27. S. Sakamoto, F. Taura, R. Tsuchihashi, W. Putalun, J. Kinjo, H. Tanaka, and S. Morimoto Expression, purification, and characterization of anti-plumbagin single-chain variable fragment antibody in Sf9 insect cell Hybridoma (Larchmt.) 29 2010 481 488
28. S.A. Shestopal, J.H. Kurasawa, T.K. Lee, and A.G. Sarafanov Expression of the light chain of coagulation factor VIII in insect cells Blood 118 2011 985
29. A. Sarafanov, and E. Saenko High-throughput optimization of protein expression in the baculovirus system based on determination of relative expression efficiency of viral stocks Anal. Biochem. 328 2004 98 100 (Pubitemid 38479627)
30. A.M. Shibeko, S.A. Woodle, T.K. Lee, and M.V. Ovanesov Unifying the mechanism of recombinant FVIIa action: dose dependence is regulated differently by tissue factor and phospholipids Blood 120 2012 891 899
31. M.J. Gourley, S.A. Shestopal, T.K. Lee, and M.V. Ovanesov Development of a continuous thrombin generation-based test to measure potency in factor VIII concentrates ASH Annu. Meeting Abstracts 118 2011 1206
32. O.A. Fadeeva, M.A. Panteleev, S.S. Karamzin, A.N. Balandina, I.V. Smirnov, and F.I. Ataullakhanov Thromboplastin immobilized on polystyrene surface exhibits kinetic characteristics close to those for the native protein and activates in vitro blood coagulation similarly to thromboplastin on fibroblasts Biochemistry (Mosc.) 75 2010 734 743
33. M.V. Ovanesov, E.G. Lopatina, E.L. Saenko, N.M. Ananyeva, L.I. Ul'yanova, O.P. Plyushch, A.A. Butilin, and F.I. Ataullakhanov Effect of factor VIII on tissue factor-initiated spatial clot growth Thromb. Haemost. 89 2003 235 242 (Pubitemid 36227273)
34. R.R. Burgess Preparing a purification summary table Methods Enzymol. 463 2009 29 34
35. S. Rosen Assay of factor VIII: C with a chromogenic substrate Scand. J. Haematol. Suppl. 40 1984 139 145 (Pubitemid 14097132)
36. R.D. Langdell, R.H. Wagner, and K.M. Brinkhous Effect of antihemophilic factor on one-stage clotting tests; a presumptive test for hemophilia and a simple one-stage antihemophilic factor assay procedure J. Lab. Clin. Med. 41 1953 637 647
37. B. Maertens, A. Spriestersbach, G.U. von, U. Roth, J. Kubicek, M. Gerrits, M. Graf, M. Liss, D. Daubert, R. Wagner, and F. Schafer Gene optimization mechanisms: a multi-gene study reveals a high success rate of full-length human proteins expressed in Escherichia coli Protein Sci. 19 2010 1312 1326
38. S. Fath, A.P. Bauer, M. Liss, A. Spriestersbach, B. Maertens, P. Hahn, C. Ludwig, F. Schafer, M. Graf, and R. Wagner Multiparameter RNA and codon optimization: a standardized tool to assess and enhance autologous mammalian gene expression PLoS ONE 6 2011 e17596
39. Y. Laroche, M. Demaeyer, J.M. Stassen, Y. Gansemans, E. Demarsin, G. Matthyssens, D. Collen, and P. Holvoet Characterization of a recombinant single-chain molecule comprising the variable domains of a monoclonal antibody specific for human fibrin fragment D-dimer J. Biol. Chem. 266 1991 16343 16349 (Pubitemid 21907802)
40. T. Kretzschmar, L. Aoustin, O. Zingel, M. Marangi, B. Vonach, H. Towbin, and M. Geiser High-level expression in insect cells and purification of secreted monomeric single-chain Fv antibodies J. Immunol. Methods 195 1996 93 101 (Pubitemid 26292692)
41. A. Todorovska, R.C. Roovers, O. Dolezal, A.A. Kortt, H.R. Hoogenboom, and P.J. Hudson Design and application of diabodies, triabodies and tetrabodies for cancer targeting J. Immunol. Methods 248 2001 47 66 (Pubitemid 32167024)
42. P. Holliger, and P.J. Hudson Engineered antibody fragments and the rise of single domains Nat. Biotechnol. 23 2005 1126 1136 (Pubitemid 41486394)