Expression of recombinant anti-E-selectin single-chain Fv antibody fragments in stably transfected insect cell lines

Journal of Immunological Methods

Volumn 212, Issue 2, 1998, Pages 149-160

  Mahiouz, Danuta L ^a,b   Aichinger, Gerald ^a   Haskard, Dorian O ^b   George, Andrew J T ^a  

^a HAMMERSMITH HOSPITAL   (United Kingdom)

^b NATIONAL HEART AND LUNG INSTITUTE   (United Kingdom)

Author keywords

Adhesion molecules; Antibody engineering; Bacterial expression; Drosophila melanogaster; E selectin; Insect expression; Refolding; Single chain Fv

Indexed keywords

BACTERIAL DNA; ENDOTHELIAL LEUKOCYTE ADHESION MOLECULE 1; IMMUNOGLOBULIN FRAGMENT; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; DROSOPHILA MELANOGASTER; GENE EXPRESSION SYSTEM; GENETIC ENGINEERING; GENETIC TRANSFECTION; MOLECULAR CLONING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION;

ANIMALS; ANTIBODY SPECIFICITY; BIOSENSING TECHNIQUES; CLONING, MOLECULAR; DROSOPHILA MELANOGASTER; E-SELECTIN; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; IMMUNOGLOBULIN FRAGMENTS; IMMUNOGLOBULIN VARIABLE REGION; PROTEIN ENGINEERING; PROTEIN FOLDING; RECOMBINANT PROTEINS;

ANIMALIA; BACTERIA (MICROORGANISMS); DROSOPHILA MELANOGASTER; ESCHERICHIA COLI; INSECTA; MELANOGASTER;

EID: 0032520725     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(98)00007-6     Document Type: Article

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