-
1
-
-
0024293979
-
Single-chain antigen-binding proteins
-
Bird, R.E., Hardman, K.D., Jacobson, J.W., Johnson, S., Kaufman, B.M., Lee, S.-M., Lee, T., Pope, S.H., Riordan, O.S. and Whitlow, M. (1988) Single-chain antigen-binding proteins. Science 242, 423-426.
-
(1988)
Science
, vol.242
, pp. 423-426
-
-
Bird, R.E.1
Hardman, K.D.2
Jacobson, J.W.3
Johnson, S.4
Kaufman, B.M.5
Lee, S.-M.6
Lee, T.7
Pope, S.H.8
Riordan, O.S.9
Whitlow, M.10
-
2
-
-
84988074679
-
Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels
-
Blum, H., Beier, H. and Gross, H.J. (1987) Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8, 93-99.
-
(1987)
Electrophoresis
, vol.8
, pp. 93-99
-
-
Blum, H.1
Beier, H.2
Gross, H.J.3
-
3
-
-
0029168386
-
Rapid, high-yield recovery of a recombinant digoxin binding single chain Fv from Escherichia coli
-
Burks, E.A. and Iverson, B.L. (1995) Rapid, high-yield recovery of a recombinant digoxin binding single chain Fv from Escherichia coli. Biotechnol. Prog. 11, 112-114.
-
(1995)
Biotechnol. Prog.
, vol.11
, pp. 112-114
-
-
Burks, E.A.1
Iverson, B.L.2
-
4
-
-
0028836167
-
Purification of bacterially expressed single chain Fv antibodies for clinical applications using metal chelate chromatography
-
Casey, J.L., Keep, P.A., Chester, K.A., Robson, L., Hawkins, R.E. and Begent, R.H.J. (1995) Purification of bacterially expressed single chain Fv antibodies for clinical applications using metal chelate chromatography. J. Immunol. Meth. 179, 105-116.
-
(1995)
J. Immunol. Meth.
, vol.179
, pp. 105-116
-
-
Casey, J.L.1
Keep, P.A.2
Chester, K.A.3
Robson, L.4
Hawkins, R.E.5
Begent, R.H.J.6
-
5
-
-
0024349836
-
A recombinant immunotoxin consisting of two antibody variable domains fused to Pseudomonas exotoxin
-
Chaudhary, V.K., Queen, C., Junghans, R.P., Waldmann, T.A., FitzGerald, D.J. and Pastan, I. (1989) A recombinant immunotoxin consisting of two antibody variable domains fused to Pseudomonas exotoxin. Nature 339, 394-397.
-
(1989)
Nature
, vol.339
, pp. 394-397
-
-
Chaudhary, V.K.1
Queen, C.2
Junghans, R.P.3
Waldmann, T.A.4
FitzGerald, D.J.5
Pastan, I.6
-
6
-
-
0025362523
-
In vivo tumor targeting of a recombinant single-chain antigen-binding protein
-
Colcher, D., Bird, R., Roselli, M., Hardman, K.D., Johnson, S., Pope, S., Dodd, S.W., Pantoliano, M.W., Milenic, D.E. and Schlom, J. (1990) In vivo tumor targeting of a recombinant single-chain antigen-binding protein. J. Natl. Cancer Inst. 82, 1191-1197.
-
(1990)
J. Natl. Cancer Inst.
, vol.82
, pp. 1191-1197
-
-
Colcher, D.1
Bird, R.2
Roselli, M.3
Hardman, K.D.4
Johnson, S.5
Pope, S.6
Dodd, S.W.7
Pantoliano, M.W.8
Milenic, D.E.9
Schlom, J.10
-
7
-
-
0028136310
-
Multimerization behaviour of single chain Fv variants for the tumour-binding antibody B72.3
-
Desplancq, D., King, D.J., Lawson, A.D.G. and Mountain, A. (1994) Multimerization behaviour of single chain Fv variants for the tumour-binding antibody B72.3. Protein Engng. 7, 1027-1033.
-
(1994)
Protein Engng.
, vol.7
, pp. 1027-1033
-
-
Desplancq, D.1
King, D.J.2
Lawson, A.D.G.3
Mountain, A.4
-
8
-
-
0027138277
-
Crystallization of single-chain Fv proteins
-
Essig, N.Z., Wood, J.F., Howard, A.J., Raag, R. and Whitlow, M. (1993) Crystallization of single-chain Fv proteins. J. Mol. Biol. 234, 897-901.
-
(1993)
J. Mol. Biol.
, vol.234
, pp. 897-901
-
-
Essig, N.Z.1
Wood, J.F.2
Howard, A.J.3
Raag, R.4
Whitlow, M.5
-
9
-
-
0022340978
-
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product
-
Evan, G.I., Lewis, G.K., Ramsay, G. and Bishop, J.M. (1985) Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol. Cell. Biol. 5, 3610-3616.
-
(1985)
Mol. Cell. Biol.
, vol.5
, pp. 3610-3616
-
-
Evan, G.I.1
Lewis, G.K.2
Ramsay, G.3
Bishop, J.M.4
-
11
-
-
0027473684
-
Human anti-self antibodies with high specificity from phage display libraries
-
Griffiths, A.D., Malmqvist, M., Marks, J.D., Bye, J.M., Embleton, M.J., McCafferty, J., Baier, M., Holliger, K.P., Gorick, B.D., Hughes-Jones, N.C., Hoogenboom, H.R. and Winter, G. (1993) Human anti-self antibodies with high specificity from phage display libraries. EMBO J. 12, 725-734.
-
(1993)
EMBO J.
, vol.12
, pp. 725-734
-
-
Griffiths, A.D.1
Malmqvist, M.2
Marks, J.D.3
Bye, J.M.4
Embleton, M.J.5
McCafferty, J.6
Baier, M.7
Holliger, K.P.8
Gorick, B.D.9
Hughes-Jones, N.C.10
Hoogenboom, H.R.11
Winter, G.12
-
12
-
-
0003448569
-
-
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA
-
Harlow, E. and Lane, D. (1988) Antibodies. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.
-
(1988)
Antibodies. A Laboratory Manual
-
-
Harlow, E.1
Lane, D.2
-
13
-
-
0025740577
-
Multi-subunit proteins on the surface of filamentous phage: Methodologies for displaying antibody (Fab) heavy and light chains
-
Hoogenboom, H.R., Griffiths, A.D., Johnson, K.S., Chiswell, D.J., Hudson. P. and Winter, G. (1991) Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains. Nucl. Acids Res. 19, 4133-4137.
-
(1991)
Nucl. Acids Res.
, vol.19
, pp. 4133-4137
-
-
Hoogenboom, H.R.1
Griffiths, A.D.2
Johnson, K.S.3
Chiswell, D.J.4
Hudson, P.5
Winter, G.6
-
14
-
-
0004198722
-
Protein engineering of antibody binding sites: Recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli
-
Huston, J.S., Levinson, D., Mudgett-Hunter, M., Tai, M.-S., Novotný, J., Margolies, M.N., Ridge, R.J., Bruccoleri, R.E., Haber, E., Crea, R. and Oppermann H. (1988) Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli. Proc. Natl. Acad. Sci. USA 85, 5879-5883.
-
(1988)
Proc. Natl. Acad. Sci. USA
, vol.85
, pp. 5879-5883
-
-
Huston, J.S.1
Levinson, D.2
Mudgett-Hunter, M.3
Tai, M.-S.4
Novotný, J.5
Margolies, M.N.6
Ridge, R.J.7
Bruccoleri, R.E.8
Haber, E.9
Crea, R.10
Oppermann, H.11
-
15
-
-
0026334521
-
Protein engineering of single-chain Fv analogs and fusion proteins
-
Huston, J.S., Mudgett-Hunter, M., Tai, M.-S., McCartney, J., Warren, F., Haber, E. and Oppermann, H. (1991) Protein engineering of single-chain Fv analogs and fusion proteins. Methods Enzymol. 203, 46-88.
-
(1991)
Methods Enzymol.
, vol.203
, pp. 46-88
-
-
Huston, J.S.1
Mudgett-Hunter, M.2
Tai, M.-S.3
McCartney, J.4
Warren, F.5
Haber, E.6
Oppermann, H.7
-
16
-
-
0027420237
-
Medical applications of single-chain antibodies
-
Huston, J.S., McCartney, J., Tai, M.-S., Mottola-Hartshorn, C., Jin, D., Warren, F., Keck, P. and Oppermann, H. (1993) Medical applications of single-chain antibodies. Intern. Rev. Immunol. 10, 195-217.
-
(1993)
Intern. Rev. Immunol.
, vol.10
, pp. 195-217
-
-
Huston, J.S.1
McCartney, J.2
Tai, M.-S.3
Mottola-Hartshorn, C.4
Jin, D.5
Warren, F.6
Keck, P.7
Oppermann, H.8
-
17
-
-
0025944815
-
Immobilization of proteins to a carboxymethyldextran-modified gold surface for biospecific interaction analysis in surface plasmon resonance sensors
-
Johnsson, B., Löfås, S. and Lindquist, G. (1991) Immobilization of proteins to a carboxymethyldextran-modified gold surface for biospecific interaction analysis in surface plasmon resonance sensors. Anal. Biochem. 198, 268-277.
-
(1991)
Anal. Biochem.
, vol.198
, pp. 268-277
-
-
Johnsson, B.1
Löfås, S.2
Lindquist, G.3
-
18
-
-
0027970870
-
Mammalian expression and secretion of functional single-chain Fv molecules
-
Jost, C.R., Kurucz, I., Jacobus, C.M., Titus, J.A., George, A.J.T. and Segal, D. (1994) Mammalian expression and secretion of functional single-chain Fv molecules. J. Biol. Chem. 269, 26267-26273.
-
(1994)
J. Biol. Chem.
, vol.269
, pp. 26267-26273
-
-
Jost, C.R.1
Kurucz, I.2
Jacobus, C.M.3
Titus, J.A.4
George, A.J.T.5
Segal, D.6
-
19
-
-
0028294931
-
Recombinant anti-sialidase single-chain variable fragment antibody. Characterization, formation of dimer and higher-molecular-mass multimers and the solution of the crystal structure of the single-chain variable fragment/sialidase complex
-
Kortt, A.A., Malby, R.L., Caldwell, J.B., Gruen, L.C., Ivancic, N., Lawrence, M.C., Hewlett, G.J., Webster, R.G., Hudson, P.J. and Colman, P.M. (1994) Recombinant anti-sialidase single-chain variable fragment antibody. Characterization, formation of dimer and higher-molecular-mass multimers and the solution of the crystal structure of the single-chain variable fragment/sialidase complex. Eur. J. Biochem. 221, 151-157.
-
(1994)
Eur. J. Biochem.
, vol.221
, pp. 151-157
-
-
Kortt, A.A.1
Malby, R.L.2
Caldwell, J.B.3
Gruen, L.C.4
Ivancic, N.5
Lawrence, M.C.6
Hewlett, G.J.7
Webster, R.G.8
Hudson, P.J.9
Colman, P.M.10
-
20
-
-
0028983802
-
Selection procedures for nonmatured phage antibodies: A quantitative comparison and optimization stratagies
-
Kretzschmar, T, Zimmermann, C. and Geiser, M. (1995) Selection procedures for nonmatured phage antibodies: a quantitative comparison and optimization stratagies. Anal. Biochem. 224, 413-419.
-
(1995)
Anal. Biochem.
, vol.224
, pp. 413-419
-
-
Kretzschmar, T.1
Zimmermann, C.2
Geiser, M.3
-
21
-
-
0028566655
-
Characterization of four nucleic acid-binding single-chain Fv fragments by direct and competitive solid-phase radioimmunoassays
-
Kuderova, A., Tanha, J. and Lee, J.S. (1994) Characterization of four nucleic acid-binding single-chain Fv fragments by direct and competitive solid-phase radioimmunoassays. J. Biol. Chem. 269, 32957-32962.
-
(1994)
J. Biol. Chem.
, vol.269
, pp. 32957-32962
-
-
Kuderova, A.1
Tanha, J.2
Lee, J.S.3
-
22
-
-
0014949207
-
Cleavage of structural proteins during the assembly of the head of bacteriophage T4
-
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
-
(1970)
Nature
, vol.227
, pp. 680-685
-
-
Laemmli, U.K.1
-
23
-
-
0025951211
-
Characterization of a recombinant single-chain molecule comprising the variable domains of a monoclonal antibody specific for human fibrin fragment D-dimer
-
Laroche, Y., Demaeyer, M., Stassen, J.-M., Gansemans, Y., Demarsin, E., Matthyssens, G., Collen, D. and Holvoet, P. (1991) Characterization of a recombinant single-chain molecule comprising the variable domains of a monoclonal antibody specific for human fibrin fragment D-dimer. J. Biol. Chem. 266, 16343-16349.
-
(1991)
J. Biol. Chem.
, vol.266
, pp. 16343-16349
-
-
Laroche, Y.1
Demaeyer, M.2
Stassen, J.-M.3
Gansemans, Y.4
Demarsin, E.5
Matthyssens, G.6
Collen, D.7
Holvoet, P.8
-
24
-
-
0018257739
-
Inheritance of antibody specificity v. Anti-2-phenyl-oxazolone in the mouse
-
Mäkelä, O., Kaartinen, M., Pelkonen, J.L.T. and Karjalainen, K. (1978) Inheritance of antibody specificity V. Anti-2-phenyl-oxazolone in the mouse. J. Exp. Med. 148, 1644-1660.
-
(1978)
J. Exp. Med.
, vol.148
, pp. 1644-1660
-
-
Mäkelä, O.1
Kaartinen, M.2
Pelkonen, J.L.T.3
Karjalainen, K.4
-
25
-
-
0026317443
-
By-passing immunization. Human antibodies from V-gene libraries displayed on phage
-
Marks, J.D., Hoogenboom, H.R., Bonnert, T.P., McCafferty, J., Griffiths, A.D. and Winter, G. (1991) By-passing immunization. Human antibodies from V-gene libraries displayed on phage. J. Mol. Biol. 222, 581-597.
-
(1991)
J. Mol. Biol.
, vol.222
, pp. 581-597
-
-
Marks, J.D.1
Hoogenboom, H.R.2
Bonnert, T.P.3
McCafferty, J.4
Griffiths, A.D.5
Winter, G.6
-
26
-
-
14744300211
-
By-passing immunization: Building high affinity human antibodies by chain shuffling
-
Marks, J.D., Griffiths, A.D., Malmqvist, M., Clackson, T.P., Bye, J.M. and Winter, G. (1992) By-passing immunization: building high affinity human antibodies by chain shuffling. Bio/Technology 10, 779-783.
-
(1992)
Bio/Technology
, vol.10
, pp. 779-783
-
-
Marks, J.D.1
Griffiths, A.D.2
Malmqvist, M.3
Clackson, T.P.4
Bye, J.M.5
Winter, G.6
-
27
-
-
0026327378
-
Construction, binding properties, metabolism and tumor targeting of a single-chain Fv derived from the pancarcinoma monoclonal antibody CC49
-
Milenic, D.E., Yokota, T., Filpula, D.R., Finkelman, M.A.J., Dodd, S.W., Wood, J.F., Whitlow, M., Snoy, P. and Schlom, J. (1991) Construction, binding properties, metabolism and tumor targeting of a single-chain Fv derived from the pancarcinoma monoclonal antibody CC49. Cancer Res. 51, 6363-6371.
-
(1991)
Cancer Res.
, vol.51
, pp. 6363-6371
-
-
Milenic, D.E.1
Yokota, T.2
Filpula, D.R.3
Finkelman, M.A.J.4
Dodd, S.W.5
Wood, J.F.6
Whitlow, M.7
Snoy, P.8
Schlom, J.9
-
28
-
-
0028939489
-
Calmodulin as a versatile tag for antibody fragments
-
Neri, D., de Lalla, C., Petrul, H., Neri, P. and Winter, G. (1995) Calmodulin as a versatile tag for antibody fragments. Bio/Technology 13, 373-377.
-
(1995)
Bio/Technology
, vol.13
, pp. 373-377
-
-
Neri, D.1
De Lalla, C.2
Petrul, H.3
Neri, P.4
Winter, G.5
-
29
-
-
0027442660
-
Improved bivalent miniantibodies, with identical avidity as whole antibodies, produced by high cell density fermentation of Escherichia coli
-
Pack, P., Kujau, M., Schroeckh, V., Knüpfer, U., Wenderoth, R., Riesenberg, D. and Plückthun, A. (1993) Improved bivalent miniantibodies, with identical avidity as whole antibodies, produced by high cell density fermentation of Escherichia coli. Bio/Technology 11, 1271-1277.
-
(1993)
Bio/Technology
, vol.11
, pp. 1271-1277
-
-
Pack, P.1
Kujau, M.2
Schroeckh, V.3
Knüpfer, U.4
Wenderoth, R.5
Riesenberg, D.6
Plückthun, A.7
-
30
-
-
0028949091
-
Tetravalent miniantibodies with high avidity assembling in Escherichia coli
-
Pack, P., Müller, K., Zahn, R. and Plückthun, A. (1995) Tetravalent miniantibodies with high avidity assembling in Escherichia coli. J. Mol. Biol. 246, 28-34.
-
(1995)
J. Mol. Biol.
, vol.246
, pp. 28-34
-
-
Pack, P.1
Müller, K.2
Zahn, R.3
Plückthun, A.4
-
31
-
-
0025788111
-
Conformational stability, folding and ligand-binding affinity of single-chain Fv immunoglobulin fragments expressed in Escherichia coli
-
Pantoliano, M.W., Bird, R.E., Johnson, S., Asel, E.D., Dodd, S.W., Wood, J.F. and Hardman, K.D. (1991) Conformational stability, folding and ligand-binding affinity of single-chain Fv immunoglobulin fragments expressed in Escherichia coli. Biochemistry 30, 10117-10125.
-
(1991)
Biochemistry
, vol.30
, pp. 10117-10125
-
-
Pantoliano, M.W.1
Bird, R.E.2
Johnson, S.3
Asel, E.D.4
Dodd, S.W.5
Wood, J.F.6
Hardman, K.D.7
-
32
-
-
0027487772
-
Antibody production in the baculovirus expression system
-
Potter, K.N., Li, Y. and Capra, J.D. (1993) Antibody production in the baculovirus expression system. Intern. Rev. Immunol. 10, 103-112.
-
(1993)
Intern. Rev. Immunol.
, vol.10
, pp. 103-112
-
-
Potter, K.N.1
Li, Y.2
Capra, J.D.3
-
33
-
-
0028843420
-
Single-chain Fvs
-
Raag, R. and Whitlow, M. (1995) Single-chain Fvs. FASEB J. 9, 73-80.
-
(1995)
FASEB J.
, vol.9
, pp. 73-80
-
-
Raag, R.1
Whitlow, M.2
-
34
-
-
0028944846
-
Generation of rabbit monoclonal antibody fragments from a combinatorial phage display library and their production in the yeast Pichia pastoris
-
Ridder, R., Schmitz, R., Legay, F. and Gram, H. (1995) Generation of rabbit monoclonal antibody fragments from a combinatorial phage display library and their production in the yeast Pichia pastoris. Bio/Technology 13, 255-260.
-
(1995)
Bio/Technology
, vol.13
, pp. 255-260
-
-
Ridder, R.1
Schmitz, R.2
Legay, F.3
Gram, H.4
-
35
-
-
0024292736
-
Assembly of a functional immunoglobulin Fv fragment in Escherichia coli
-
Skerra, A. and Plückthun, A. (1988) Assembly of a functional immunoglobulin Fv fragment in Escherichia coli. Science 240, 1038-1041.
-
(1988)
Science
, vol.240
, pp. 1038-1041
-
-
Skerra, A.1
Plückthun, A.2
-
36
-
-
14744267241
-
The functional expression of antibody Fv fragments in Escherichia coli: Improved vectors and a generally applicable purification technique
-
Skerra, A., Pfitzinger, I. and Plückthun, A. (1991) The functional expression of antibody Fv fragments in Escherichia coli: improved vectors and a generally applicable purification technique. Bio/Technology 9, 273-278.
-
(1991)
Bio/Technology
, vol.9
, pp. 273-278
-
-
Skerra, A.1
Pfitzinger, I.2
Plückthun, A.3
-
37
-
-
0026153423
-
Quantitative determination of surface concentration of protein with surface plasmon resonance using radiolabeled proteins
-
Stenberg, E., Persson, B., Roos, H. and Urbaniczky, C. (1991) Quantitative determination of surface concentration of protein with surface plasmon resonance using radiolabeled proteins. J. Colloid Interface Sci. 143, 513-526.
-
(1991)
J. Colloid Interface Sci.
, vol.143
, pp. 513-526
-
-
Stenberg, E.1
Persson, B.2
Roos, H.3
Urbaniczky, C.4
-
38
-
-
0027332881
-
An improved linker for single-chain Fv with reduced aggregation and enhanced proteolytic stability
-
Whitlow, M., Bell, B.A., Feng, S.-L., Filpula, D., Hardman, K.D., Hubert, S.L., Rollence, M.L., Wood, J.F., Schott, M.E., Milenic, D.E., Yokota, T. and Schlom, J. (1993) An improved linker for single-chain Fv with reduced aggregation and enhanced proteolytic stability. Prot. Engng. 6, 989-995.
-
(1993)
Prot. Engng.
, vol.6
, pp. 989-995
-
-
Whitlow, M.1
Bell, B.A.2
Feng, S.-L.3
Filpula, D.4
Hardman, K.D.5
Hubert, S.L.6
Rollence, M.L.7
Wood, J.F.8
Schott, M.E.9
Milenic, D.E.10
Yokota, T.11
Schlom, J.12
-
39
-
-
0025978976
-
Man-made antibodies
-
Winter, G. and Milstein, C. (1991) Man-made antibodies. Nature 349, 293-299.
-
(1991)
Nature
, vol.349
, pp. 293-299
-
-
Winter, G.1
Milstein, C.2
-
40
-
-
0027962758
-
Making antibodies by phage display technology
-
Winter, G., Griffiths, A.D., Hawkins, R.E. and Hoogenboom, H.R. (1994) Making antibodies by phage display technology. Annu. Rev. Immunol. 12, 433-455.
-
(1994)
Annu. Rev. Immunol.
, vol.12
, pp. 433-455
-
-
Winter, G.1
Griffiths, A.D.2
Hawkins, R.E.3
Hoogenboom, H.R.4
-
41
-
-
0027078548
-
Genetically engineered antibodies: Progress and prospects
-
Wright, A., Shin, S.-U. and Morrison, S.L. (1992) Genetically engineered antibodies: progress and prospects. Crit. Rev. Immunol. 12, 125-168.
-
(1992)
Crit. Rev. Immunol.
, vol.12
, pp. 125-168
-
-
Wright, A.1
Shin, S.-U.2
Morrison, S.L.3
-
42
-
-
14744300958
-
Efficient production of a functional single-chain antidigoxin antibody via an engineered Bacillus subtilis expression-secretion system
-
Wu, X.-C, Ng, S.-C., Near, R.I. and Wong, S.-L. (1993) Efficient production of a functional single-chain antidigoxin antibody via an engineered Bacillus subtilis expression-secretion system. Bio/Technology 11, 71-76.
-
(1993)
Bio/Technology
, vol.11
, pp. 71-76
-
-
Wu, X.-C.1
Ng, S.-C.2
Near, R.I.3
Wong, S.-L.4
-
43
-
-
0026684815
-
Rapid tumor penetration of a single-chain Fv and comparison with other immunoglobulin forms
-
Yokota, T., Milenic, D.E., Whitlow, M. and Schlom, J. (1992) Rapid tumor penetration of a single-chain Fv and comparison with other immunoglobulin forms. Cancer Res. 52, 3402-3408.
-
(1992)
Cancer Res.
, vol.52
, pp. 3402-3408
-
-
Yokota, T.1
Milenic, D.E.2
Whitlow, M.3
Schlom, J.4
|