High-throughput optimization of protein expression in the baculovirus system based on determination of relative expression efficiency of viral stocks

Analytical Biochemistry

Volumn 328, Issue 1, 2004, Pages 98-100

  Sarafanov, Andrey ^a   Saenko, Evgueni ^a  

^a AMERICAN RED CROSS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY;

BACULOVIRUS SYSTEM; EXPRESSION EFFICIENCY; HIGH THROUGHPUT; PROTEIN EXPRESSIONS;

CHEMISTRY;

VIRUS PROTEIN;

ARTICLE; BACULOVIRUS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEOMICS; REACTION OPTIMIZATION; VIRUS TITRATION;

UNIDENTIFIED BACULOVIRUS;

EID: 1842786993     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2003.11.028     Document Type: Article

References (11)

1. King L.A., Possee R.D. The Baculovirus Expression System: A Laboratory Guide. 1992;Chapman & Hall, London.
2. Hink W.F., Vail P.V. A plaque assay for titration alfalfa looper nuclear polyhedrosis virus in a cabbage looper TN-368 cell line. J. Invertebr. Pathol. 22:1973;168-174.
3. O'Reilly D.R., Miller L.K., Luckow V.A. Baculovirus Expression Vectors: A Laboratory Manual. 1992;W.H. Freeman and Company, New York.
4. Grosse F., Manns A. Fast isolation of recombinant baculovirus by antibody screening. Richardson C.D. Methods in Molecular Biology. vol. 39:1995;179-185 Humana Press, Totowa, NJ.
5. Cha H.J., Gotoh T., Bentley W.E. Simplification of titer determination for recombinant baculovirus by green fluorescent protein marker. Biotechniques. 23:1997;782-786.
6. Katagiri Y., Ingham K.C. Enhanced production of green fluorescent fusion proteins in a baculovirus expression system by addition of secretion signal. Biotechniques. 33:2002;24-26.
7. Koszelak M.E., Huggins C.F., Fay P.J. Sites in the A2 subunit involved in the interfactor VIIIa interaction. J. Biol. Chem. 275:2000;27137-27144.
8. Sarafanov A.G., Ananyeva N.M., Shima M., Saenko E.L. Cell surface heparan sulfate proteoglycans participate in factor VIII catabolism mediated by low density lipoprotein receptor-related protein. J. Biol. Chem. 276:2001;11970-11979.
9. Saenko E.L., Yakhyaev A.V., Mikhailenko I., Strickland D.K., Sarafanov A.G. Role of the low density lipoprotein-related protein receptor in mediation of factor VIII catabolism. J. Biol. Chem. 274:1999;37685-37692.
10. Liebman J.M., LaSala D., Wang W., Steed P.M. When less is more: enhanced baculovirus production of recombinant proteins at very low multiplicities of infection. Biotechniques. 26:1999;36-42.
11. Garzia L., Andre A., Amoresano A., D'Angelo A., Martusciello R., Cirulli C., Tsurumi T., Marino G., Zollo M. Method to express and purify nm23-H2 protein from baculovirus-infected cells. Biotechniques. 35:2003;384-391.